IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Molecular structural and functional characterization of STAT1 gene regulatory region in teleost Channa argus
Jia, Weizhang; Zhou, Xiuxia; Jia, WZ, Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China
2010
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume135Issue:40180Pages:146-151
AbstractThe transcription factor STAT1 is involved in signal transduction of type I and II interferons (IFNs). However, the molecular characteristics of the STAT1 regulatory region still remain to be elucidated in teleosts. In the present study, the complete cDNA and the regulatory region of the STAT1 gene were isolated from snakehead (Channa argus). More than 2.4 kb 5'-flanking region of STAT1 shares the regulatory elements of IFN-stimulated response element (ISRE) and IFN-gamma activation site (GAS). Consensus ISRE and GAS were located from -373 to -361 and -716 to -724 in the promoter region, respectively. Moreover, it is noticeable that the crucial elements of ISRE (+698 to +710) and GAS (+294 and +301) are present in the first intron of snakehead STAT1. Comparisons of six vertebrate STAT1 5'-flanking regions all present the common sequence characteristics of IFN-induced gene promoter, which include ISRE, GAS and Sp1 sites. In order to further characterize the snakehead STAT1 regulatory region, six reporter constructs of snakehead STAT1 promoter and first intron were generated to examine the specificity to human interferon-gamma (hIFN-gamma). Only those constructs containing the ISRE element showed notable reporter activity after stimulation of Hela cells with hIFN-gamma. However, sequential deletions of putative transcription factor binding sites indicated that GAS elements have little effect on the promoter and intronic activity in response to hIFN-gamma. Taken together, these results suggest that the regulatory mechanisms of IFN-signalling appear to be mediated in a similar manner in fish and mammals. (C) 2009 Elsevier B.V. All rights reserved.
KeywordInterferon Stat1 Regulatory Region Isre Luciferase Activity
Department[Jia, Weizhang] Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China; [Zhou, Xiuxia] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022]
Indexed BySCI
Language英语
WOS IDWOS:000277557600016
Funding OrganizationNational Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091]; Scientific Research Startup of Guangdong Pharmaceutical University [43555022]
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13854
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorJia, WZ, Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Jia, Weizhang,Zhou, Xiuxia,Jia, WZ, Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China. Molecular structural and functional characterization of STAT1 gene regulatory region in teleost Channa argus[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2010,135(40180):146-151.
APA Jia, Weizhang,Zhou, Xiuxia,&Jia, WZ, Guangdong Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Guangzhou 510006, Guangdong, Peoples R China.(2010).Molecular structural and functional characterization of STAT1 gene regulatory region in teleost Channa argus.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,135(40180),146-151.
MLA Jia, Weizhang,et al."Molecular structural and functional characterization of STAT1 gene regulatory region in teleost Channa argus".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 135.40180(2010):146-151.
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