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CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120
He, Dongli; Xu, Xudong; Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2010
Source PublicationARCHIVES OF MICROBIOLOGY
ISSN0302-8933
Volume192Issue:6Pages:461-469
AbstractA protein of cyAbrB family of regulators, Alr0946 (CalA), was identified by DNA affinity chromatography using DNA fragments from the promoter region of ftsZ in Anabaena/Nostoc sp. PCC 7120. Electrophoretic mobility shift assays showed that the protein could bind to upstream regions of ftsZ (as biotin-labeled DNA fragments) and that the binding was inhibited by the same DNA fragments and a fragment upstream of hetC but not by 3 fragments upstream of glnA, hetR or ntcA. Both transcriptional fusion with gfp (green fluorescence protein) and Western blot analysis indicated that the expression of calA was down-regulated in heterocysts relative to vegetative cells. An insertional mutant of calA could not be completely segregated. Over-expression of calA in Anabaena/Nostoc caused no change in growth, heterocyst differentiation or expression of ftsZ. CalA as a DNA-binding protein is essential for the growth of Anabaena/Nostoc and may be required for the expression of ftsZ in vegetative cells.
KeywordSp-strain Pcc-7120
Department[He, Dongli; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Subject AreaMicrobiology
Funding OrganizationNational Natural Science Foundation of China [30570022, 30770049] ; National Natural Science Foundation of China [30570022, 30770049] ; National Natural Science Foundation of China [30570022, 30770049] ; National Natural Science Foundation of China [30570022, 30770049]
Indexed BySCI
Language英语
WOS IDWOS:000277785300005
Funding OrganizationNational Natural Science Foundation of China [30570022, 30770049] ; National Natural Science Foundation of China [30570022, 30770049] ; National Natural Science Foundation of China [30570022, 30770049] ; National Natural Science Foundation of China [30570022, 30770049]
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13789
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
He, Dongli,Xu, Xudong,Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China. CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120[J]. ARCHIVES OF MICROBIOLOGY,2010,192(6):461-469.
APA He, Dongli,Xu, Xudong,&Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.(2010).CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120.ARCHIVES OF MICROBIOLOGY,192(6),461-469.
MLA He, Dongli,et al."CalA, a cyAbrB protein, binds to the upstream region of ftsZ and is down-regulated in heterocysts in Anabaena sp PCC 7120".ARCHIVES OF MICROBIOLOGY 192.6(2010):461-469.
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