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Characterization of a Monomeric Heat-Labile Classical Alkaline Phosphatase from Anabaena sp PCC7120
Luo, Ming; Guo, Yong-Chao; Deng, Jiao-Yu; Wei, Hong-Ping; Zhang, Zhi-Ping; Leng, Yan; Men, Dong; Song, Li-Rong; Zhang, Xian-En; Zhou, Ya-Feng; Zhou, YF, Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
2010
Source PublicationBIOCHEMISTRY-MOSCOW
ISSN0006-2979
Volume75Issue:5Pages:655-664
AbstractAlkaline phosphatases (APs), known inducible enzymes of the Pho regulon and poorly characterized in cyanobacteria, hydrolyze phosphomonoesters to produce inorganic phosphate (P-i) during P-i starvation. In this study, two predicted alkaline phosphatase genes in the genome of Anabaena sp. PCC 7120, all2843 and alr5291, were apparently induced during P-i starvation. Sequence analysis showed that alr5291 encodes a protein that is an atypical alkaline phosphatase like other cyanobacteria PhoAs, but the protein encoded by all2843 is very similar to the classical PhoAs, such as Escherichia coli alkaline phosphatase (EAP). To date, there have been no reports about classical phoA in cyanobacterial genomes. The alkaline phosphatase AP(A), coded by all2843, is characterized as a metalloenzyme containing Mg2+ and Zn2+ with molar ratio of 1 : 2. Site-directed mutagenesis analysis indicated that, though the active center of AP(A) is highly conserved in comparison with EAP, differences do exist between AP(A) and EAP in metal ion coordination. Besides, biochemical analysis revealed that AP(A) is a monomeric protein and inactivated rapidly at 50 degrees C. These results suggest that AP(A) is the first monomeric heat-labile classical PhoA found in cyanobacteria.
KeywordAnabaena Sp Pcc7120 Phosphorous Starvation Alkaline Phosphatase Metalloenzyme Site-directed Mutagenesis
Department[Luo, Ming; Guo, Yong-Chao; Deng, Jiao-Yu; Wei, Hong-Ping; Zhang, Zhi-Ping; Leng, Yan; Men, Dong; Zhang, Xian-En; Zhou, Ya-Feng] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China; [Luo, Ming; Leng, Yan; Men, Dong] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Song, Li-Rong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds ; National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds ; National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds ; National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds
Indexed BySCI
Language英语
WOS IDWOS:000279568800017
Funding OrganizationNational Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds ; National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds ; National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds ; National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13785
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorZhou, YF, Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
Recommended Citation
GB/T 7714
Luo, Ming,Guo, Yong-Chao,Deng, Jiao-Yu,et al. Characterization of a Monomeric Heat-Labile Classical Alkaline Phosphatase from Anabaena sp PCC7120[J]. BIOCHEMISTRY-MOSCOW,2010,75(5):655-664.
APA Luo, Ming.,Guo, Yong-Chao.,Deng, Jiao-Yu.,Wei, Hong-Ping.,Zhang, Zhi-Ping.,...&Zhou, YF, Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.(2010).Characterization of a Monomeric Heat-Labile Classical Alkaline Phosphatase from Anabaena sp PCC7120.BIOCHEMISTRY-MOSCOW,75(5),655-664.
MLA Luo, Ming,et al."Characterization of a Monomeric Heat-Labile Classical Alkaline Phosphatase from Anabaena sp PCC7120".BIOCHEMISTRY-MOSCOW 75.5(2010):655-664.
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