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学科主题: Biochemistry & Molecular Biology
题名: Characterization of a Monomeric Heat-Labile Classical Alkaline Phosphatase from Anabaena sp PCC7120
作者: Luo, Ming1, 2; Guo, Yong-Chao1; Deng, Jiao-Yu1; Wei, Hong-Ping1; Zhang, Zhi-Ping1; Leng, Yan1, 2; Men, Dong1, 2; Song, Li-Rong3; Zhang, Xian-En1; Zhou, Ya-Feng1
通讯作者: Zhou, YF, Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
关键词: Anabaena sp PCC7120 ; phosphorous starvation ; alkaline phosphatase ; metalloenzyme ; site-directed mutagenesis
刊名: BIOCHEMISTRY-MOSCOW
发表日期: 2010-05-01
DOI: 10.1134/S0006297910050172
卷: 75, 期:5, 页:655-664
收录类别: SCI
文章类型: Article
部门归属: [Luo, Ming; Guo, Yong-Chao; Deng, Jiao-Yu; Wei, Hong-Ping; Zhang, Zhi-Ping; Leng, Yan; Men, Dong; Zhang, Xian-En; Zhou, Ya-Feng] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China; [Luo, Ming; Leng, Yan; Men, Dong] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Song, Li-Rong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China [2008CB418000]; Chinese Academy of Sciences ; State Key Laboratory Funds
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Alkaline phosphatases (APs), known inducible enzymes of the Pho regulon and poorly characterized in cyanobacteria, hydrolyze phosphomonoesters to produce inorganic phosphate (P-i) during P-i starvation. In this study, two predicted alkaline phosphatase genes in the genome of Anabaena sp. PCC 7120, all2843 and alr5291, were apparently induced during P-i starvation. Sequence analysis showed that alr5291 encodes a protein that is an atypical alkaline phosphatase like other cyanobacteria PhoAs, but the protein encoded by all2843 is very similar to the classical PhoAs, such as Escherichia coli alkaline phosphatase (EAP). To date, there have been no reports about classical phoA in cyanobacterial genomes. The alkaline phosphatase AP(A), coded by all2843, is characterized as a metalloenzyme containing Mg2+ and Zn2+ with molar ratio of 1 : 2. Site-directed mutagenesis analysis indicated that, though the active center of AP(A) is highly conserved in comparison with EAP, differences do exist between AP(A) and EAP in metal ion coordination. Besides, biochemical analysis revealed that AP(A) is a monomeric protein and inactivated rapidly at 50 degrees C. These results suggest that AP(A) is the first monomeric heat-labile classical PhoA found in cyanobacteria.
英文摘要: Alkaline phosphatases (APs), known inducible enzymes of the Pho regulon and poorly characterized in cyanobacteria, hydrolyze phosphomonoesters to produce inorganic phosphate (P(i)) during P(i) starvation. In this study, two predicted alkaline phosphatase genes in the genome of Anabaena sp. PCC 7120, all2843 and alr5291, were apparently induced during P(i) starvation. Sequence analysis showed that alr5291 encodes a protein that is an atypical alkaline phosphatase like other cyanobacteria PhoAs, but the protein encoded by all2843 is very similar to the classical PhoAs, such as Escherichia coli alkaline phosphatase (EAP). To date, there have been no reports about classical phoA in cyanobacterial genomes. The alkaline phosphatase AP(A), coded by all2843, is characterized as a metalloenzyme containing Mg(2+) and Zn(2+) with molar ratio of 1 : 2. Site-directed mutagenesis analysis indicated that, though the active center of AP(A) is highly conserved in comparison with EAP, differences do exist between AP(A) and EAP in metal ion coordination. Besides, biochemical analysis revealed that AP(A) is a monomeric protein and inactivated rapidly at 50 degrees C. These results suggest that AP(A) is the first monomeric heat-labile classical PhoA found in cyanobacteria.
关键词[WOS]: STRAIN PCC 7942 ; ESCHERICHIA-COLI ; ACTIVE-SITE ; PHOSPHORUS LIMITATION ; CRYSTAL-STRUCTURE ; CATALYTIC-ACTIVITY ; REACTION-MECHANISM ; ATLANTIC-OCEAN ; ZINC-BINDING ; SYSTEM
语种: 英语
WOS记录号: WOS:000279568800017
ISSN号: 0006-2979
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13785
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Luo, Ming; Guo, Yong-Chao; Deng, Jiao-Yu; Wei, Hong-Ping; Zhang, Zhi-Ping; Leng, Yan; Men, Dong; Song, Li-Rong; Zhang, Xian-En; Zhou, Ya-Feng.Characterization of a Monomeric Heat-Labile Classical Alkaline Phosphatase from Anabaena sp PCC7120,BIOCHEMISTRY-MOSCOW,2010,75(5):655-664
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