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Sulforaphane protects Microcystin-LR-induced toxicity through activation of the Nrf2-mediated defensive response
Gan, Nanqin; Mi, Lixin; Sun, Xiaoyun; Dai, Guofei; Chung, Fung-Lung; Song, Lirong; Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Beijing, Peoples R China
2010
Source PublicationTOXICOLOGY AND APPLIED PHARMACOLOGY
ISSN0041-008X
Volume247Issue:2Pages:129-137
AbstractMicrocystins (MCs), a cyclic heptapeptide hepatotoxins. are mainly produced by the bloom-forming cyanobacerium Microcystis, which has become an environmental hazard worldwide. Long term consumption of MC-contaminated water may induce liver damage, liver cancer, and even human death. Therefore, in addition to removal of MCs in drinking water, novel strategies that prevent health damages are urgently needed. Sulforaphane (SFN), a natural-occurring isothiocyanate from cruciferous vegetables, has been reported to reduce and eliminate toxicities from xenobiotics and carcinogens. The purpose of the present study was to provide mechanistic insights into the SFN-induced antioxidative defense system against MC-LR-induced cytotoxicity. We performed cell viability assays, including MTS assay, colony formation assay and apoptotic cell sorting, to study MC-LR-induced cellular damage and the protective effects by SFN. The results showed that SFN protected MC-LR-induced damages at a nontoxic and physiological relevant dose in HepG2, BRL-3A and NIH 3 T3 cells. The protection was Nrf2-mediated as evident by transactivation of Nrf2 and activation of its downstream genes, including NQO1 and HO-1, and elevated intracellular GSH level. Results of our studies indicate that pretreatment of cells with 10 mu M SFN for 12 h significantly protected cells from MC-LR-induced damage. SFN-induced protective response was mediated through Nrf2 pathway. (C) 2010 Elsevier Inc. All rights reserved.
KeywordMicrocystin-lr (Mc-lr) Sulforaphane (Sfn) Nf-e2-related Factor 2 (Nrf2) Phase Ii Enzymes Glutathione (Gsh) Chemoprevention
Department[Gan, Nanqin; Sun, Xiaoyun; Dai, Guofei; Song, Lirong] CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Beijing, Peoples R China; [Mi, Lixin; Chung, Fung-Lung] Georgetown Univ, Lombardi Comprehens Canc Ctr, Dept Oncol, Washington, DC 20057 USA
Subject AreaPharmacology & Pharmacy ; Toxicology
Funding Organization973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005] ; 973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005] ; 973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005] ; 973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005]
Indexed BySCI
Language英语
WOS IDWOS:000280903000008
Funding Organization973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005] ; 973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005] ; 973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005] ; 973 program [2008CB418000]; Natural Science Foundation of China [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]; National water pollution control and management technology [2009ZX07527-005]
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13779
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorSong, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Gan, Nanqin,Mi, Lixin,Sun, Xiaoyun,et al. Sulforaphane protects Microcystin-LR-induced toxicity through activation of the Nrf2-mediated defensive response[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2010,247(2):129-137.
APA Gan, Nanqin.,Mi, Lixin.,Sun, Xiaoyun.,Dai, Guofei.,Chung, Fung-Lung.,...&Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Beijing, Peoples R China.(2010).Sulforaphane protects Microcystin-LR-induced toxicity through activation of the Nrf2-mediated defensive response.TOXICOLOGY AND APPLIED PHARMACOLOGY,247(2),129-137.
MLA Gan, Nanqin,et al."Sulforaphane protects Microcystin-LR-induced toxicity through activation of the Nrf2-mediated defensive response".TOXICOLOGY AND APPLIED PHARMACOLOGY 247.2(2010):129-137.
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