IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry
Gan NanQin; Huang Qun; Zheng LingLing; Song LiRong; Song, LR, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2010
Source PublicationSCIENCE CHINA-LIFE SCIENCES
Volume53Issue:8Pages:973-980
AbstractToxic cyanobacterial blooms constitute a threat to human safety because Microcystis sp. releases microcystins during growth, and particularly during cell death. Therefore, analysis of toxic and nontoxic Microcystis in natural communities is required in order to assess and predict bloom dynamics and toxin production by these organisms. In this study, an analysis combining fluorescence in situ hybridization (FISH) with flow cytometry (FCM) was used to discriminate between toxic and nontoxic Microcystis and also to quantify the percentage of toxic Microcystis present in blooms. The results demonstrate that the combination of FISH and flow cytometry is a useful approach for studying the ecology of Microcystis toxin production and for providing an early warning for toxic Microcystis blooms.
KeywordCyanobacteria Bloom Toxic-microcystis Fluorescence In Situ Hybridization (Fish) Flow Cytometry (Fcm)
Department[Gan NanQin; Huang Qun; Zheng LingLing; Song LiRong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiology
Funding OrganizationNational Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]
Indexed BySCI
WOS IDWOS:000281670300008
Funding OrganizationNational Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1] ; National Basic Research Program of China [2008CB418000]; National Water Pollution Control and Management Technology Major Project [2009ZX07527-005]; Natural Science Foundation of China-Yunnan [U0833604]; Chinese Academy of Sciences [KZCX1-YW-14-1]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13775
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorSong, LR, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Gan NanQin,Huang Qun,Zheng LingLing,et al. Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(8):973-980.
APA Gan NanQin,Huang Qun,Zheng LingLing,Song LiRong,&Song, LR, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2010).Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry.SCIENCE CHINA-LIFE SCIENCES,53(8),973-980.
MLA Gan NanQin,et al."Quantitative assessment of toxic and nontoxic Microcystis colonies in natural environments using fluorescence in situ hybridization and flow cytometry".SCIENCE CHINA-LIFE SCIENCES 53.8(2010):973-980.
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