cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)

	cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)
	Tan, Wenhua; Liu, Yang; Wu, Zhongxing; Lin, Shen; Yu, Gongliang; Yu, Boshi; Li, Renhui; Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China
	2010
Source Publication	HARMFUL ALGAE
Volume	9 Issue:6 Pages:607-612
Abstract	Microcystis wesenbergii (Komarek) Komarek in Kondrateva, a major bloom forming cyanobacterial species, possesses unique colonial characteristics which can be easily distinguished from other Microcystis species. However, there is still no genetic marker to effectively characterize M. wesenbergii. In this research, thirteen strains of M. wesenbergii, collected from eight locations in Chinese water bodies were examined for molecular characterization of both cpcBA-IGS sequences (phycocyanin intergenic spacer and flanking regions) and ITS sequences (internal transcribed spacer region between 16S and 235 rDNA). The phylogenetic analysis based on cpcBA-IGS sequences showed that the M. wesenbergii strains formed a distinct cluster with high support values, indicating the cpcBA-IGS region could be used to characterize and distinguish M. wesenbergii from other species of Microcystis. These developed primers were verified to be effective in distinguishing M. wesenbergii from other species of Microcystis and from other species in different genera of cyanobacteria. (c) 2010 Elsevier B.V. All rights reserved.
Keyword	Cpcba-igs Cyanobacteria Its Microcystis Wesenbergii Phylogeny Water Bloom
Department	[Tan, Wenhua; Liu, Yang; Wu, Zhongxing; Lin, Shen; Yu, Gongliang; Yu, Boshi; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Hubei, Peoples R China; [Tan, Wenhua; Liu, Yang; Lin, Shen] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject Area	Marine & Freshwater Biology
Funding Organization	State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1]
Indexed By	SCI
WOS ID	WOS:000281999300010
Funding Organization	State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1]
Citation statistics	Cited Times:17[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.ihb.ac.cn/handle/342005/13771
Collection	藻类生物学及应用研究中心_期刊论文
Corresponding Author	Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China
Recommended Citation GB/T 7714	Tan, Wenhua,Liu, Yang,Wu, Zhongxing,et al. cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)[J]. HARMFUL ALGAE,2010,9(6):607-612.
APA	Tan, Wenhua.,Liu, Yang.,Wu, Zhongxing.,Lin, Shen.,Yu, Gongliang.,...&Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China.(2010).cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria).HARMFUL ALGAE,9(6),607-612.
MLA	Tan, Wenhua,et al."cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)".HARMFUL ALGAE 9.6(2010):607-612.

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