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学科主题: Marine & Freshwater Biology
题名: cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)
作者: Tan, Wenhua1, 2; Liu, Yang1, 2; Wu, Zhongxing1; Lin, Shen1, 2; Yu, Gongliang1; Yu, Boshi1; Li, Renhui1
通讯作者: Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China
关键词: cpcBA-IGS ; Cyanobacteria ; ITS ; Microcystis wesenbergii ; Phylogeny ; Water bloom
刊名: HARMFUL ALGAE
发表日期: 2010-09-01
DOI: 10.1016/j.hal.2010.04.011
卷: 9, 期:6, 页:607-612
收录类别: SCI
文章类型: Article
部门归属: [Tan, Wenhua; Liu, Yang; Wu, Zhongxing; Lin, Shen; Yu, Gongliang; Yu, Boshi; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Hubei, Peoples R China; [Tan, Wenhua; Liu, Yang; Lin, Shen] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1]
类目[WOS]: Marine & Freshwater Biology
研究领域[WOS]: Marine & Freshwater Biology
摘要: Microcystis wesenbergii (Komarek) Komarek in Kondrateva, a major bloom forming cyanobacterial species, possesses unique colonial characteristics which can be easily distinguished from other Microcystis species. However, there is still no genetic marker to effectively characterize M. wesenbergii. In this research, thirteen strains of M. wesenbergii, collected from eight locations in Chinese water bodies were examined for molecular characterization of both cpcBA-IGS sequences (phycocyanin intergenic spacer and flanking regions) and ITS sequences (internal transcribed spacer region between 16S and 235 rDNA). The phylogenetic analysis based on cpcBA-IGS sequences showed that the M. wesenbergii strains formed a distinct cluster with high support values, indicating the cpcBA-IGS region could be used to characterize and distinguish M. wesenbergii from other species of Microcystis. These developed primers were verified to be effective in distinguishing M. wesenbergii from other species of Microcystis and from other species in different genera of cyanobacteria. (c) 2010 Elsevier B.V. All rights reserved.
英文摘要: Microcystis wesenbergii (Komarek) Komarek in Kondrateva, a major bloom forming cyanobacterial species, possesses unique colonial characteristics which can be easily distinguished from other Microcystis species. However, there is still no genetic marker to effectively characterize M. wesenbergii. In this research, thirteen strains of M. wesenbergii, collected from eight locations in Chinese water bodies were examined for molecular characterization of both cpcBA-IGS sequences (phycocyanin intergenic spacer and flanking regions) and ITS sequences (internal transcribed spacer region between 16S and 235 rDNA). The phylogenetic analysis based on cpcBA-IGS sequences showed that the M. wesenbergii strains formed a distinct cluster with high support values, indicating the cpcBA-IGS region could be used to characterize and distinguish M. wesenbergii from other species of Microcystis. These developed primers were verified to be effective in distinguishing M. wesenbergii from other species of Microcystis and from other species in different genera of cyanobacteria. (c) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: RIBOSOMAL-RNA SEQUENCES ; GENUS MICROCYSTIS ; PHYLOGENETIC-RELATIONSHIPS ; GENETIC DIVERSITY ; LAKE WANNSEE ; WATER BODIES ; STRAINS ; CYANOPHYTA ; AERUGINOSA ; 16S
语种: 英语
WOS记录号: WOS:000281999300010
ISSN号: 1568-9883
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13771
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Tan, Wenhua; Liu, Yang; Wu, Zhongxing; Lin, Shen; Yu, Gongliang; Yu, Boshi; Li, Renhui.cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria),HARMFUL ALGAE,2010,9(6):607-612
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