cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria) | |
Tan, Wenhua; Liu, Yang; Wu, Zhongxing; Lin, Shen; Yu, Gongliang; Yu, Boshi; Li, Renhui; Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China | |
2010 | |
Source Publication | HARMFUL ALGAE
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Volume | 9Issue:6Pages:607-612 |
Abstract | Microcystis wesenbergii (Komarek) Komarek in Kondrateva, a major bloom forming cyanobacterial species, possesses unique colonial characteristics which can be easily distinguished from other Microcystis species. However, there is still no genetic marker to effectively characterize M. wesenbergii. In this research, thirteen strains of M. wesenbergii, collected from eight locations in Chinese water bodies were examined for molecular characterization of both cpcBA-IGS sequences (phycocyanin intergenic spacer and flanking regions) and ITS sequences (internal transcribed spacer region between 16S and 235 rDNA). The phylogenetic analysis based on cpcBA-IGS sequences showed that the M. wesenbergii strains formed a distinct cluster with high support values, indicating the cpcBA-IGS region could be used to characterize and distinguish M. wesenbergii from other species of Microcystis. These developed primers were verified to be effective in distinguishing M. wesenbergii from other species of Microcystis and from other species in different genera of cyanobacteria. (c) 2010 Elsevier B.V. All rights reserved. |
Keyword | Cpcba-igs Cyanobacteria Its Microcystis Wesenbergii Phylogeny Water Bloom |
Department | [Tan, Wenhua; Liu, Yang; Wu, Zhongxing; Lin, Shen; Yu, Gongliang; Yu, Boshi; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Hubei, Peoples R China; [Tan, Wenhua; Liu, Yang; Lin, Shen] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
Subject Area | Marine & Freshwater Biology |
Funding Organization | State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] |
Indexed By | SCI |
WOS ID | WOS:000281999300010 |
Funding Organization | State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] ; State Key Basic Research and Development Plan of China [2008CB418002]; National Water Science and Technology [2008ZX07105-006, 2008ZX07101-013]; Chinese Academy of Science [082303-1-501, KZCX1-YW-14-1] |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/342005/13771 |
Collection | 藻类生物学及应用研究中心_期刊论文 |
Corresponding Author | Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China |
Recommended Citation GB/T 7714 | Tan, Wenhua,Liu, Yang,Wu, Zhongxing,et al. cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)[J]. HARMFUL ALGAE,2010,9(6):607-612. |
APA | Tan, Wenhua.,Liu, Yang.,Wu, Zhongxing.,Lin, Shen.,Yu, Gongliang.,...&Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China.(2010).cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria).HARMFUL ALGAE,9(6),607-612. |
MLA | Tan, Wenhua,et al."cpcBA-IGS as an effective marker to characterize Microcystis wesenbergii (Komarek) Komarek in Kondrateva (cyanobacteria)".HARMFUL ALGAE 9.6(2010):607-612. |
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