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学科主题: Plant Sciences; Marine & Freshwater Biology
题名: UNRAVELING MOLECULAR DIVERSITY AND PHYLOGENY OF APHANIZOMENON (NOSTOCALES, CYANOBACTERIA) STRAINS ISOLATED FROM CHINA1
作者: Wu, Zhongxing1; Shi, Junqiong2; Lin, Shen3; Li, Renhui1
通讯作者: Li, RH, Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Peoples R China
关键词: 16S rRNA ; Anabaena ; Aphanizomenon ; cpcBA-IGS ; rbcLX
刊名: JOURNAL OF PHYCOLOGY
发表日期: 2010-10-01
DOI: 10.1111/j.1529-8817.2010.00869.x
卷: 46, 期:5, 页:1048-1058
收录类别: SCI
文章类型: Article
部门归属: [Wu, Zhongxing; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Peoples R China; [Shi, Junqiong] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Herts, Peoples R China; [Lin, Shen] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: State Key Basic Research and Development Plan of China [2008CB418002]; National Natural Science Foundation of China [30800123, 30770165]; Chinese Academy of Sciences [082303-1-501, KZCX1-YW-14-1]
类目[WOS]: Plant Sciences ; Marine & Freshwater Biology
研究领域[WOS]: Plant Sciences ; Marine & Freshwater Biology
摘要: Fifty-three strains of the genus Aphanizomenon isolated from Chinese waters were employed to conduct morphological examination and sequencing of the 16S rRNA gene, rbcLX (RUBISCO), and cpcBA-IGS gene regions. Based on morphological characteristics, the examined strains were divided into three morphotypes [Aph. flos-aquae Breb. ex Bornet et Flahault, Aph. gracile Lemmerm., and Aph. issatchenkoi (Usacer) Proshk.-Lavr.]. Phylogenetic analysis based on 16S rRNA and rbcLX showed that Aphanizomenon strains could be divided into three main clades (Clade A of Aph. flos-aquae, Clade B of Aph. gracile, and Clade C of Aph. issatchenkoi), but two additional clades formed by Aph. ovalisporum and Aph. aphanizomenoides were detected in the 16S rDNA-based topology. All Aph. issatchenkoi strains contained an additional 175 nucleotides from the 779 to 954 nucleotide location in rbcLX region, compared with strains of Aph. flos-aquae and Aph. gracile. The cpcBA-IGS-based phylogenetic tree revealed that Aph. issatchenkoi strains were not discriminated from Aph. flos-aquae strains; however, a concatenated alignment of 16S rDNA, rbcLX, and cpcBA-IGS led to the three distinct clades (Aph. flos-aquae, Aph. gracile, and Aph. issatchenkoi, respectively). It is suggested that the taxonomic revision of Aphanizomenon and Anabaena genera is required to be performed by employing multilocus sequence analysis and polyphasic studies.
英文摘要: Fifty-three strains of the genus Aphanizomenon isolated from Chinese waters were employed to conduct morphological examination and sequencing of the 16S rRNA gene, rbcLX (RUBISCO), and cpcBA-IGS gene regions. Based on morphological characteristics, the examined strains were divided into three morphotypes [Aph. flos-aquae Breb. ex Bornet et Flahault, Aph. gracile Lemmerm., and Aph. issatchenkoi (Usacer) Proshk.-Lavr.]. Phylogenetic analysis based on 16S rRNA and rbcLX showed that Aphanizomenon strains could be divided into three main clades (Clade A of Aph. flos-aquae, Clade B of Aph. gracile, and Clade C of Aph. issatchenkoi), but two additional clades formed by Aph. ovalisporum and Aph. aphanizomenoides were detected in the 16S rDNA-based topology. All Aph. issatchenkoi strains contained an additional 175 nucleotides from the 779 to 954 nucleotide location in rbcLX region, compared with strains of Aph. flos-aquae and Aph. gracile. The cpcBA-IGS-based phylogenetic tree revealed that Aph. issatchenkoi strains were not discriminated from Aph. flos-aquae strains; however, a concatenated alignment of 16S rDNA, rbcLX, and cpcBA-IGS led to the three distinct clades (Aph. flos-aquae, Aph. gracile, and Aph. issatchenkoi, respectively). It is suggested that the taxonomic revision of Aphanizomenon and Anabaena genera is required to be performed by employing multilocus sequence analysis and polyphasic studies.
关键词[WOS]: 16S RIBOSOMAL-RNA ; PHYCOCYANIN INTERGENIC SPACER ; PARALYTIC SHELLFISH TOXINS ; FLOS-AQUAE NH-5 ; BALTIC SEA ; MICROCYSTIS-AERUGINOSA ; POPULATION-STRUCTURE ; SEQUENCE ALIGNMENT ; GENERA ANABAENA ; 1ST REPORT
语种: 英语
WOS记录号: WOS:000282378200023
ISSN号: 0022-3646
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13767
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat Biol, Wuhan 430072, Peoples R China
2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Herts, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Wu, Zhongxing; Shi, Junqiong; Lin, Shen; Li, Renhui.UNRAVELING MOLECULAR DIVERSITY AND PHYLOGENY OF APHANIZOMENON (NOSTOCALES, CYANOBACTERIA) STRAINS ISOLATED FROM CHINA1,JOURNAL OF PHYCOLOGY,2010,46(5):1048-1058
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文件名: UNRAVELING MOLECULAR DIVERSITY AND PHYLOGENY OF APHANIZOMENON (NOSTOCALES, CYANOBACTERIA) STRAINS ISOLATED FROM CHINA1.pdf
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