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学科主题: Ecology; Environmental Sciences; Toxicology
题名: Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses
作者: Shao, Jihai1, 2, 3; Yu, Gongliang1; Wang, Zhongjie1, 3; Wu, Zhongxing1; Peng, Xin1; Li, Renhui1
通讯作者: Li, RH, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Wheat bran leachate ; Microcystis ; Gene expression ; Photosynthesis
刊名: ECOTOXICOLOGY
发表日期: 2010-11-01
DOI: 10.1007/s10646-010-0549-1
卷: 19, 期:8, 页:1634-1641
收录类别: SCI
文章类型: Article
部门归属: [Shao, Jihai; Yu, Gongliang; Wang, Zhongjie; Wu, Zhongxing; Peng, Xin; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Shao, Jihai] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China; [Shao, Jihai; Wang, Zhongjie] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China (NSFC) [30821140542]; National Water Science and Technology Projects [2009ZX07101-013-02, 2008ZX07105-006]; Chinese Academy of Sciences [082303-1-501]
类目[WOS]: Ecology ; Environmental Sciences ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology
摘要: Wheat bran leachate (WBL) has been shown to have an inhibitory effect on Microcystis aeruginosa in this study. In order to explore the inhibitory mechanism of WBL on M. aeruginosa, physiological responses of M. aeruginosa NIES-843 under the WBL stress were studied. The expressions of six important genes related to the D1 protein of photosynthetic processes (psbA), synthesis of microcystins (mcyB), antioxidant protein peroxiredoxin (prx), synthesis of fatty acid (fabZ) and the repair of biological macromolecules (recA, grpE) were studied. Under the WBL stress, no significant change was found in expressions of both grpE and recA, but expressions of psbA, fabZ and prx were shown to be down-regulated, and slight up-regulated expression was found in mcyB. It was shown that oxygen evolution of M. aeruginosa NIES-843 was significantly depressed, and intracellular ATP contents became lower, after exposure to WBL. Similarly, maximum electron transport rates of photosynthetic activities decreased significantly, but intracellular reactive oxygen species levels boosted dramatically under the WBL stress, and cell lysis was observed. Therefore, it is suggested that photosynthetic systems and membranes were the potential targets of toxicity of WBL on M. aeruginosa, and the oxidative damage is an important mechanism explaining the inhibitory effect of WBL on M. aeruginosa.
英文摘要: Wheat bran leachate (WBL) has been shown to have an inhibitory effect on Microcystis aeruginosa in this study. In order to explore the inhibitory mechanism of WBL on M. aeruginosa, physiological responses of M. aeruginosa NIES-843 under the WBL stress were studied. The expressions of six important genes related to the D1 protein of photosynthetic processes (psbA), synthesis of microcystins (mcyB), antioxidant protein peroxiredoxin (prx), synthesis of fatty acid (fabZ) and the repair of biological macromolecules (recA, grpE) were studied. Under the WBL stress, no significant change was found in expressions of both grpE and recA, but expressions of psbA, fabZ and prx were shown to be down-regulated, and slight up-regulated expression was found in mcyB. It was shown that oxygen evolution of M. aeruginosa NIES-843 was significantly depressed, and intracellular ATP contents became lower, after exposure to WBL. Similarly, maximum electron transport rates of photosynthetic activities decreased significantly, but intracellular reactive oxygen species levels boosted dramatically under the WBL stress, and cell lysis was observed. Therefore, it is suggested that photosynthetic systems and membranes were the potential targets of toxicity of WBL on M. aeruginosa, and the oxidative damage is an important mechanism explaining the inhibitory effect of WBL on M. aeruginosa.
关键词[WOS]: BARLEY-STRAW ; OXIDATIVE STRESS ; ANTIALGAL ACTIVITY ; GROWTH-INHIBITION ; ESCHERICHIA-COLI ; GENE-EXPRESSION ; PHOTOSYSTEM-II ; ALGAL GROWTH ; PEROXIREDOXINS ; QUANTIFICATION
语种: 英语
WOS记录号: WOS:000284363800027
ISSN号: 0963-9292
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13757
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

Recommended Citation:
Shao, Jihai; Yu, Gongliang; Wang, Zhongjie; Wu, Zhongxing; Peng, Xin; Li, Renhui.Towards clarification of the inhibitory mechanism of wheat bran leachate on Microcystis aeruginosa NIES-843 (cyanobacteria): physiological responses,ECOTOXICOLOGY,2010,19(8):1634-1641
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