中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
学科主题: Toxicology
题名: Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through Combined Inactivation of PDR and CWP Genes
作者: Zhang, Min2; Hanna, Michelle1; Li, Jia1; Butcher, Susan1; Dai, Heping2; Xiao, Wei1
通讯作者: Xiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada
关键词: Saccharomyces cerevisiae ; RNR3-lacZ ; genotoxicity test ; permeability ; ABC transporters ; cell wall
刊名: TOXICOLOGICAL SCIENCES
发表日期: 2010-02-01
DOI: 10.1093/toxsci/kfp267
卷: 113, 期:2, 页:401-411
收录类别: SCI
文章类型: Article
部门归属: [Hanna, Michelle; Li, Jia; Butcher, Susan; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada; [Zhang, Min; Dai, Heping] Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002]
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
摘要: We previously established a genotoxicity detection system based on the transcriptional response of the yeast RNR3 gene to DNA damage. In order to further improve its sensitivity to genotoxicants, we have attempted to increase cell permeability by removing cell wall mannoproteins (CWPs). Here, we report that selected deletion of pleiotropic drug resistance (PDR) genes encoding membrane efflux transporters also enhanced cellular sensitivity to treatment by various genotoxic agents. Furthermore, we have validated our hypothesis that PDR and CWP protect cells through different mechanisms by demonstrating that simultaneous inactivation of the above two pathways resulted in a synergistic enhancement of assay sensitivity as measured by RNR3-lacZ expression and that this effect is at the cell permeability level. The quadruple mutation results in RNR3-lacZ assay sensitivity to tested chemicals that apparently surpasses the industry standard Ames test. We argue that this hyperpermeable yeast mutant strain would be suitable for other chemical-based genotoxic assays.
英文摘要: We previously established a genotoxicity detection system based on the transcriptional response of the yeast RNR3 gene to DNA damage. In order to further improve its sensitivity to genotoxicants, we have attempted to increase cell permeability by removing cell wall mannoproteins (CWPs). Here, we report that selected deletion of pleiotropic drug resistance (PDR) genes encoding membrane efflux transporters also enhanced cellular sensitivity to treatment by various genotoxic agents. Furthermore, we have validated our hypothesis that PDR and CWP protect cells through different mechanisms by demonstrating that simultaneous inactivation of the above two pathways resulted in a synergistic enhancement of assay sensitivity as measured by RNR3-lacZ expression and that this effect is at the cell permeability level. The quadruple mutation results in RNR3-lacZ assay sensitivity to tested chemicals that apparently surpasses the industry standard Ames test. We argue that this hyperpermeable yeast mutant strain would be suitable for other chemical-based genotoxic assays.
关键词[WOS]: PROMOTER-REPORTER CONSTRUCT ; SACCHAROMYCES-CEREVISIAE ; MULTIDRUG-RESISTANCE ; DNA-DAMAGE ; PLEIOTROPIC DRUG ; TRANSCRIPTIONAL CONTROL ; ABC TRANSPORTERS ; TESTING SYSTEM ; IN-VIVO ; SENSITIVITY
语种: 英语
WOS记录号: WOS:000273706200014
ISSN号: 1096-6080
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13755
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through.pdf(283KB)----开放获取--View Download

作者单位: 1.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
2.Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Zhang, Min; Hanna, Michelle; Li, Jia; Butcher, Susan; Dai, Heping; Xiao, Wei.Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through Combined Inactivation of PDR and CWP Genes,TOXICOLOGICAL SCIENCES,2010,113(2):401-411
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Min]'s Articles
[Hanna, Michelle]'s Articles
[Li, Jia]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Min]‘s Articles
[Hanna, Michelle]‘s Articles
[Li, Jia]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace