IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through Combined Inactivation of PDR and CWP Genes
Zhang, Min; Hanna, Michelle; Li, Jia; Butcher, Susan; Dai, Heping; Xiao, Wei; Xiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada
2010
Source PublicationTOXICOLOGICAL SCIENCES
ISSN1096-6080
Volume113Issue:2Pages:401-411
AbstractWe previously established a genotoxicity detection system based on the transcriptional response of the yeast RNR3 gene to DNA damage. In order to further improve its sensitivity to genotoxicants, we have attempted to increase cell permeability by removing cell wall mannoproteins (CWPs). Here, we report that selected deletion of pleiotropic drug resistance (PDR) genes encoding membrane efflux transporters also enhanced cellular sensitivity to treatment by various genotoxic agents. Furthermore, we have validated our hypothesis that PDR and CWP protect cells through different mechanisms by demonstrating that simultaneous inactivation of the above two pathways resulted in a synergistic enhancement of assay sensitivity as measured by RNR3-lacZ expression and that this effect is at the cell permeability level. The quadruple mutation results in RNR3-lacZ assay sensitivity to tested chemicals that apparently surpasses the industry standard Ames test. We argue that this hyperpermeable yeast mutant strain would be suitable for other chemical-based genotoxic assays.
KeywordSaccharomyces Cerevisiae Rnr3-lacz Genotoxicity Test Permeability Abc Transporters Cell Wall
Department[Hanna, Michelle; Li, Jia; Butcher, Susan; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada; [Zhang, Min; Dai, Heping] Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Hubei, Peoples R China
Subject AreaToxicology
Funding OrganizationNatural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002] ; Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002] ; Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002] ; Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002]
Indexed BySCI
Language英语
WOS IDWOS:000273706200014
Funding OrganizationNatural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002] ; Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002] ; Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002] ; Natural Science Foundation of China [30440420603]; Natural Sciences and Engineering Research Council of Canada [138338]; Chinese National Key Laboratory FEBT [2008FB002]
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13755
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorXiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada
Recommended Citation
GB/T 7714
Zhang, Min,Hanna, Michelle,Li, Jia,et al. Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through Combined Inactivation of PDR and CWP Genes[J]. TOXICOLOGICAL SCIENCES,2010,113(2):401-411.
APA Zhang, Min.,Hanna, Michelle.,Li, Jia.,Butcher, Susan.,Dai, Heping.,...&Xiao, W, Univ Saskatchewan, Dept Microbiol & Immunol, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada.(2010).Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through Combined Inactivation of PDR and CWP Genes.TOXICOLOGICAL SCIENCES,113(2),401-411.
MLA Zhang, Min,et al."Creation of a Hyperpermeable Yeast Strain to Genotoxic Agents through Combined Inactivation of PDR and CWP Genes".TOXICOLOGICAL SCIENCES 113.2(2010):401-411.
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