IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19
Wan, Xiaoyang; Ji, Wei; Mei, Xue; Zhou, Jiangang; Liu, Jing-xia; Fang, Chengchi; Xiao, Wuhan; Wan, XY, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
2010
Source PublicationPLOS ONE
ISSN1932-6203
Volume5Issue:2Pages:-
AbstractPrevious studies indicated that EAF (ELL-associated factor) family members, EAF1 and EAF2/U19, play a role in cancer and embryogenesis. For example, EAF2/U19 may serve as a tumor suppressor in prostate cancer. At the same time, EAF2/U19 is a downstream factor in the non-canonical Wnt4 signaling pathway required for eye development in Xenopus laevis, and along with EAF1, contributes to convergence and extension movements in zebrafish embryos through Wnt maintenance. Here, we used zebrafish embryos and mammalian cells to show that both EAF1 and EAF2/U19 were up-regulated by Wnt4 (Wnt4a). Furthermore, we found that EAF1 and EAF2/U19 suppressed Wnt4 expression by directly binding to the Wnt4 promoter as seen in chromatin immunoprecipitation assays. These findings indicate that an auto-regulatory negative feedback loop occurs between Wnt4 and the EAF family, which is conserved between zebrafish and mammalian. The rescue experiments in zebrafish embryos showed that early embryonic development required the maintenance of the appropriate levels of Wnt4a through the feedback loop. Others have demonstrated that the tumor suppressors p63, p73 and WT1 positively regulate Wnt4 expression while p21 has the opposite effect, suggesting that maintenance of appropriate Wnt4 expression may also be critical for adult tissue homeostasis and prevention against tumor initiation. Thus, the autoregulatory negative feedback loop that controls expression of Wnt4 and EAF proteins may play an important role in both embryonic development and tumor suppression. Our findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo.
KeywordExtension Movements
Department[Wan, Xiaoyang; Ji, Wei; Mei, Xue; Zhou, Jiangang; Liu, Jing-xia; Fang, Chengchi; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
Subject AreaBiology ; Multidisciplinary Sciences
Funding OrganizationNSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404] ; NSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404] ; NSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404] ; NSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404]
Indexed BySCI
Language英语
WOS IDWOS:000274442600016
Funding OrganizationNSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404] ; NSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404] ; NSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404] ; NSFC [30671037, 30971667, 30700440, 20890113]; National Transgene Project [2009ZX08010-021B]; [2010CB126306]; [2007CB815705]; [2004CB117404]
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13753
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorWan, XY, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Wan, Xiaoyang,Ji, Wei,Mei, Xue,et al. Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19[J]. PLOS ONE,2010,5(2):-.
APA Wan, Xiaoyang.,Ji, Wei.,Mei, Xue.,Zhou, Jiangang.,Liu, Jing-xia.,...&Wan, XY, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China.(2010).Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19.PLOS ONE,5(2),-.
MLA Wan, Xiaoyang,et al."Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19".PLOS ONE 5.2(2010):-.
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