E2F-1 Directly Regulates Thrombospondin 1 Expression

	E2F-1 Directly Regulates Thrombospondin 1 Expression
	Ji, Wei; Zhang, Wei; Xiao, Wuhan; Ji, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
	2010
Source Publication	PLOS ONE
Volume	5 Pages:-e13442
Abstract	Thrombospondin 1 (TSP1) has been shown to play a critical role in inhibiting angiogenesis, resulting in inhibition of tumor growth and metastases. To figure out TSP1's regulators will lead to reveal its biological function mechanistically. In this study, we show that E2F-1 could activate the transcription of TSP1 by both promoter assays and Northern blot. Analysis of various TSP1 promoter mutant constructs showed that a sequence located -144/-137 up-stream of the transcriptional initiation site, related to the consensus E2F-responsive sequence, is necessary for the activation. In consistence with up-regulation of TSP-1 activity by over-expression of E2F-1, the knockdown of endogenous E2F-1 inhibited TSP-1 promoter activity significantly, implying that E2F-1 mediated regulation of TSP-1 is relevant in vivo. In addition, E2F-1 could also directly bind to the TSP1 promoter region covering -144/-137 region as revealed by ChIP assays. Furthermore, the E2F-1-induced activation of TSP1 gene transcription is suppressed by pRB1 in a dose-dependent manner. Taken together, the results demonstrate that TSP1 is a novel target for E2F1, which might imply that E2F-1 can affect angiogenesis by modulating TSP1 expression.
Keyword	Cell-cycle Control Endothelial-cells Rb/e2f Pathway Leukemia Mice Angiogenesis Gene Mll Apoptosis Cd36
Department	[Ji, Wei; Zhang, Wei; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
Funding Organization	973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Indexed By	SCI
Language	英语
WOS ID	WOS:000283043700027
Funding Organization	973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Citation statistics	Cited Times:16[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.ihb.ac.cn/handle/342005/13751
Collection	水生生物分子与细胞生物学研究中心_期刊论文
Corresponding Author	Ji, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
Recommended Citation GB/T 7714	Ji, Wei,Zhang, Wei,Xiao, Wuhan,et al. E2F-1 Directly Regulates Thrombospondin 1 Expression[J]. PLOS ONE,2010,5:-e13442.
APA	Ji, Wei,Zhang, Wei,Xiao, Wuhan,&Ji, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China.(2010).E2F-1 Directly Regulates Thrombospondin 1 Expression.PLOS ONE,5,-e13442.
MLA	Ji, Wei,et al."E2F-1 Directly Regulates Thrombospondin 1 Expression".PLOS ONE 5(2010):-e13442.

Files in This Item:
File Name/Size	DocType	Version	Access	License
E2F-1 Directly Regul（1236KB）			开放获取	--	View Application Full Text

File name:	E2F-1 Directly Regulates Thrombospondin 1 Expression.pdf
Format:	Adobe PDF

If you have any objections to this item, please fill out the form below and the administrator will contact you as soon as possible.
Content:
Email：	*
Institution:
Verification Code:	Refresh

Any comments and suggestions are welcomed.
Title:	*
Content:
Email：	*
Verification Code:	Refresh

中国科学院水生生物研究所机构知识库