IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
E2F-1 Directly Regulates Thrombospondin 1 Expression
Ji, Wei; Zhang, Wei; Xiao, Wuhan; Ji, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
2010
Source PublicationPLOS ONE
Volume5Pages:-e13442
AbstractThrombospondin 1 (TSP1) has been shown to play a critical role in inhibiting angiogenesis, resulting in inhibition of tumor growth and metastases. To figure out TSP1's regulators will lead to reveal its biological function mechanistically. In this study, we show that E2F-1 could activate the transcription of TSP1 by both promoter assays and Northern blot. Analysis of various TSP1 promoter mutant constructs showed that a sequence located -144/-137 up-stream of the transcriptional initiation site, related to the consensus E2F-responsive sequence, is necessary for the activation. In consistence with up-regulation of TSP-1 activity by over-expression of E2F-1, the knockdown of endogenous E2F-1 inhibited TSP-1 promoter activity significantly, implying that E2F-1 mediated regulation of TSP-1 is relevant in vivo. In addition, E2F-1 could also directly bind to the TSP1 promoter region covering -144/-137 region as revealed by ChIP assays. Furthermore, the E2F-1-induced activation of TSP1 gene transcription is suppressed by pRB1 in a dose-dependent manner. Taken together, the results demonstrate that TSP1 is a novel target for E2F1, which might imply that E2F-1 can affect angiogenesis by modulating TSP1 expression.
KeywordCell-cycle Control Endothelial-cells Rb/e2f Pathway Leukemia Mice Angiogenesis Gene Mll Apoptosis Cd36
Department[Ji, Wei; Zhang, Wei; Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
Funding Organization973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Indexed BySCI
Language英语
WOS IDWOS:000283043700027
Funding Organization973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; 973'' grant [2010CB126306, 2007CB815705, 2006CB504102]; Natural Science Foundation of China (NSFC) [31071211, 30971667, 30890113]; National Transgene Project [2009ZX08010-021B] W.X. is supported by 973'' grant 2010CB126306, 2007CB815705, 2006CB504102, Natural Science Foundation of China (NSFC) grant 31071211, 30971667, 30890113 and National Transgene Project 2009ZX08010-021B. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13751
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorJi, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Ji, Wei,Zhang, Wei,Xiao, Wuhan,et al. E2F-1 Directly Regulates Thrombospondin 1 Expression[J]. PLOS ONE,2010,5:-e13442.
APA Ji, Wei,Zhang, Wei,Xiao, Wuhan,&Ji, W, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan, Peoples R China.(2010).E2F-1 Directly Regulates Thrombospondin 1 Expression.PLOS ONE,5,-e13442.
MLA Ji, Wei,et al."E2F-1 Directly Regulates Thrombospondin 1 Expression".PLOS ONE 5(2010):-e13442.
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