IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells
Liu, Lingqi; Ai, Junkui; Xiao, Wuhan; Liu, June; Wang, Yujuan; Xin, Dianqi; He, Zhisong; Guo, Yinglu; Wang, Zhou; He, ZS, Peking Univ, Hosp 1, Dept Urol, 8 Xishiku St, Beijing 100034, Peoples R China
2010
Source PublicationPROSTATE
Volume70Issue:7Pages:797-805
AbstractBACKGROUND. Eleven nineteen lysine-rich leukemia (ELL) plays an important role in tumorigenesis and animal development. HIF-1 is a transcriptional factor that functions as a master regulator of O-2 homeostasis. Our previous studies showed that a binding partner of ELL, U19/Eaf2, can modulate HIF-1 alpha activity and hypoxia response, suggesting that ELL may also influence HIF-1 alpha pathway and hypoxia response. METHODS. Co-localization and co-immunoprecipitation were performed to test the interaction between ELL and HIF-1 alpha. PC3 cells with stable ELL knockdown and PC3 cells with stable ELL overexpression, along with their controls, were established using lentiviral expression system. Western blot and real-time PCR were performed to test the effect of ELL on HIF-1 alpha protein and its down-stream gene transcription. To elucidate potential effect of hypoxia on ELL, cell growth and colony formation assays were performed using PC3 subline with stable ELL overexpression. RESULTS. ELL is associated with HIF-1 alpha in transfected cells. In PC3 prostate cancer cells, ELL inhibited HIF-1 alpha protein level and down-stream gene expression. As expected, ELL inhibited cell growth and colony formation under normoxia. Interestingly, the inhibition was alleviated under hypoxia. CONCLUSIONS. Our findings suggest that ELL and HIF-1 alpha are binding partners and can modulate the functions of each other in hypoxia. Prostate 70: 797-805, 2010. (C) 2010 Wiley-Liss, Inc.
KeywordEll Hypoxia Hif-1 Alpha Prostate Cancer
Department[Liu, Lingqi; Xin, Dianqi; He, Zhisong; Guo, Yinglu] Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China; [Liu, Lingqi; Xin, Dianqi; He, Zhisong; Guo, Yinglu] Peking Univ, Inst Urol, Beijing 100034, Peoples R China; [Liu, Lingqi; Ai, Junkui; Liu, June; Wang, Zhou] Univ Pittsburgh, Sch Med, Dept Urol, Pittsburgh, PA 15232 USA; [Xiao, Wuhan] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Wang, Yujuan] Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15232 USA; [Wang, Zhou] Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Pittsburgh, PA 15232 USA; [Wang, Zhou] Univ Pittsburgh, Sch Med, Inst Canc, Pittsburgh, PA 15232 USA
Subject AreaEndocrinology & Metabolism ; Urology & Nephrology
Funding OrganizationNIH [R37 DK51193, R01 CA120386, P50 CA90386] ; NIH [R37 DK51193, R01 CA120386, P50 CA90386] ; NIH [R37 DK51193, R01 CA120386, P50 CA90386] ; NIH [R37 DK51193, R01 CA120386, P50 CA90386]
Indexed BySCI
WOS IDWOS:000277338800012
Funding OrganizationNIH [R37 DK51193, R01 CA120386, P50 CA90386] ; NIH [R37 DK51193, R01 CA120386, P50 CA90386] ; NIH [R37 DK51193, R01 CA120386, P50 CA90386] ; NIH [R37 DK51193, R01 CA120386, P50 CA90386]
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Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13749
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorHe, ZS, Peking Univ, Hosp 1, Dept Urol, 8 Xishiku St, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lingqi,Ai, Junkui,Xiao, Wuhan,et al. ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells[J]. PROSTATE,2010,70(7):797-805.
APA Liu, Lingqi.,Ai, Junkui.,Xiao, Wuhan.,Liu, June.,Wang, Yujuan.,...&He, ZS, Peking Univ, Hosp 1, Dept Urol, 8 Xishiku St, Beijing 100034, Peoples R China.(2010).ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells.PROSTATE,70(7),797-805.
MLA Liu, Lingqi,et al."ELL is an HIF-1 alpha Partner That Regulates and Responds to Hypoxia Response in PC3 Cells".PROSTATE 70.7(2010):797-805.
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