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In vivo alternative assessment of the chemicals that interfere with anterior pituitary POMC expression and interrenal steroidogenesis in POMC: EGFP transgenic zebrafish
Sun, Lingli; Xu, Wei; He, Jiangyan; Yin, Zhan; Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2010
Source PublicationTOXICOLOGY AND APPLIED PHARMACOLOGY
ISSN0041-008X
Volume248Issue:3Pages:217-225
AbstractAdrenocorticotropin (ACTH) has been considered a classic adrenocorticotropic hormone and the key pituitary-derived peptide controlling steroidogenesis in the adult adrenal. ACTH is encoded by the propiomelanocortin (POMC) gene, and its active form is mainly synthesized and processed from the POMC-encoded multihormone precursor in the anterior pituitary. The ACTH level has always been precisely controlled in the signaling cascade of the hypothalamo-pituitary-adrenal (HPA) axis due to its central role. The purpose of this study was to investigate whether the transgenic zebrafish line with EGFP driven by the POMC promoter can be used as a surrogate marker to detect the interference effects on anterior pituitary POMC expression caused by chemicals in teleost. The Tg (POMC:EGFP) fish treated for 4 days with the known adrenergic agents, dexamethasone (Dex) or aminoglutethimide (AG), exhibited altered levels of EGFP and POMC expression in the anterior domain of pituitary corticotrophs. Whole-mount in situ hybridization revealed impaired patterns of expression of the zebrafish ftz-fl gene (ffl b), a key molecular marker for early interrenal development. Next, several chemicals and six commonly used organophosphorus compounds (OPs) were tested for their effects on anterior pituitary POMC expression and early interrenal development. Our preliminary screening analyses indicated that simazine and 3,3',4,4'5-pentachlorobiphenyl (PCB126) could interfere with anterior pituitary POMC expression and interrenal development in fish. In summary, our results demonstrated that the Tg (POMC:EGFP) zebrafish line might be employed as a specific and reproductive in vivo assessment model for the effects of endocrine disruption on HPA signaling. (C) 2010 Elsevier Inc. All rights reserved.
KeywordEndocrine Disruption Zebrafish Pomc:Egfp Transgenic Line Interrenal Development
Department[Sun, Lingli; Xu, Wei; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Sun, Lingli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject AreaPharmacology & Pharmacy ; Toxicology
Funding OrganizationHi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013]
Indexed BySCI
Language英语
WOS IDWOS:000283203600007
Funding OrganizationHi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013] ; Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20897013]
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13747
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Sun, Lingli,Xu, Wei,He, Jiangyan,et al. In vivo alternative assessment of the chemicals that interfere with anterior pituitary POMC expression and interrenal steroidogenesis in POMC: EGFP transgenic zebrafish[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2010,248(3):217-225.
APA Sun, Lingli,Xu, Wei,He, Jiangyan,Yin, Zhan,&Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.(2010).In vivo alternative assessment of the chemicals that interfere with anterior pituitary POMC expression and interrenal steroidogenesis in POMC: EGFP transgenic zebrafish.TOXICOLOGY AND APPLIED PHARMACOLOGY,248(3),217-225.
MLA Sun, Lingli,et al."In vivo alternative assessment of the chemicals that interfere with anterior pituitary POMC expression and interrenal steroidogenesis in POMC: EGFP transgenic zebrafish".TOXICOLOGY AND APPLIED PHARMACOLOGY 248.3(2010):217-225.
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