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学科主题: Developmental Biology
题名: Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish
作者: Mo, Saijun1, 2; Wang, Lu1, 2; Li, Qing1, 2; Li, Jie1, 2; Li, Yuanyuan1, 2; Thannickal, Victor J.3; Cui, Zongbin1, 2
通讯作者: Cui, ZB, Chinese Acad Sci, Inst Hydrobiol, 7 S Donghu Rd, Wuhan 430072, Hubei, Peoples R China
关键词: Caveolin-1 ; beta-catenin ; Dorsoventral patterning ; Wnt signaling ; Zebrafish
刊名: DEVELOPMENTAL BIOLOGY
发表日期: 2010-08-01
DOI: 10.1016/j.ydbio.2010.04.033
卷: 344, 期:1, 页:210-223
收录类别: SCI
文章类型: Article
部门归属: [Mo, Saijun; Wang, Lu; Li, Qing; Li, Jie; Li, Yuanyuan; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Mo, Saijun; Wang, Lu; Li, Qing; Li, Jie; Li, Yuanyuan; Cui, Zongbin] Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Wuhan 430072, Hubei, Peoples R China; [Thannickal, Victor J.] Univ Alabama, Div Pulm Allergy & Crit Care Med, Birmingham, AL USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China [2009CB941200]; National Natural Science Foundation of China [30671602, 30871442]; National Institute of Health, USA [R01 HL067967]
类目[WOS]: Developmental Biology
研究领域[WOS]: Developmental Biology
摘要: Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remains not well understood what controls dorsal activity of maternal beta-catenin and how Cav-1 functions in zebrafish embryogenesis Here, we report that Cav-1 is required for proper dorsoventral patterning in zebrafish Both Win and BMP signals act coordinately to negatively control transcriptional expression of cav-1 during embryonic development. Ectopic expression of Cav-1 alpha or -1 beta resulted in formation of typical ventralized embryos, whereas Cav-1 knockdown led to abnormal embryos with expanded expression of dorsal genes Cav-1 overexpression disrupts the nuclear translocation of beta-catenin through the interaction of its scaffolding domain with Cav-1 binding motif of beta-catenin This reciprocal interaction is necessary for the ventralizing activity of Cav-1 We have further demonstrated that human Cav-1 proteins have conserved ventralizing activity in zebrafish embryogenesis. Thus, maternally expressed zebrafish Cav-1 regulates dorsoventral patterning by limiting nuclear translocation of active beta-catenin (C) 2010 Published by Elsevier Inc
英文摘要: Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling Activation of the Wnt/beta-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remains not well understood what controls dorsal activity of maternal beta-catenin and how Cav-1 functions in zebrafish embryogenesis Here, we report that Cav-1 is required for proper dorsoventral patterning in zebrafish Both Win and BMP signals act coordinately to negatively control transcriptional expression of cav-1 during embryonic development. Ectopic expression of Cav-1 alpha or -1 beta resulted in formation of typical ventralized embryos, whereas Cav-1 knockdown led to abnormal embryos with expanded expression of dorsal genes Cav-1 overexpression disrupts the nuclear translocation of beta-catenin through the interaction of its scaffolding domain with Cav-1 binding motif of beta-catenin This reciprocal interaction is necessary for the ventralizing activity of Cav-1 We have further demonstrated that human Cav-1 proteins have conserved ventralizing activity in zebrafish embryogenesis. Thus, maternally expressed zebrafish Cav-1 regulates dorsoventral patterning by limiting nuclear translocation of active beta-catenin (C) 2010 Published by Elsevier Inc
关键词[WOS]: WNT SIGNALING PATHWAY ; AXIS FORMATION ; IN-VIVO ; FUNCTIONAL INTERACTION ; INDUCED ANTAGONIST ; PROTEIN-COMPONENT ; MEMBRANE DOMAINS ; XENOPUS EMBRYOS ; EXPRESSION ; ORGANIZER
语种: 英语
WOS记录号: WOS:000280426400019
ISSN号: 0012-1606
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13745
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Wuhan 430072, Hubei, Peoples R China
3.Univ Alabama, Div Pulm Allergy & Crit Care Med, Birmingham, AL USA

Recommended Citation:
Mo, Saijun; Wang, Lu; Li, Qing; Li, Jie; Li, Yuanyuan; Thannickal, Victor J.; Cui, Zongbin.Caveolin-1 regulates dorsoventral patterning through direct interaction with beta-catenin in zebrafish,DEVELOPMENTAL BIOLOGY,2010,344(1):210-223
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