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学科主题: Biology; Multidisciplinary Sciences
题名: Quantitative Phosphoproteomics of Proteasome Inhibition in Multiple Myeloma Cells
作者: Ge, Feng1, 2, 3; Xiao, Chuan-Le1, 2; Bi, Li-Jun4; Tao, Sheng-Ce5; Xiong, Sheng1, 2; Yin, Xin-Feng1, 2; Li, Li-Ping1, 2; Lu, Chun-Hua1, 2; Jia, Hai-Tao1, 2; He, Qing-Yu1, 2
通讯作者: Ge, F, Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China
关键词: PROTEIN-KINASE CK2 ; MICROTUBULE DYNAMICS ; SIGNALING NETWORKS ; AMINO-ACIDS ; PHOSPHORYLATION ; AUTOPHAGY ; PS-341 ; PROTEOMICS ; APOPTOSIS ; BORTEZOMIB
刊名: PLOS ONE
发表日期: 2010-09-29
DOI: 10.1371/journal.pone.0013095
卷: 5, 期:9, 页:-
收录类别: SCI
文章类型: Article
部门归属: [Ge, Feng; Xiao, Chuan-Le; Xiong, Sheng; Yin, Xin-Feng; Li, Li-Ping; Lu, Chun-Hua; Jia, Hai-Tao; He, Qing-Yu] Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China; [Ge, Feng; Xiao, Chuan-Le; Xiong, Sheng; Yin, Xin-Feng; Li, Li-Ping; Lu, Chun-Hua; Jia, Hai-Tao; He, Qing-Yu] Jinan Univ, Natl Engn Res Ctr Genet Med, Guangzhou, Guangdong, Peoples R China; [Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Bi, Li-Jun] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China; [Tao, Sheng-Ce] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200030, Peoples R China
WOS标题词: Science & Technology
资助者: Chang-Jiang Scholars Program ;
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Background: The proteasome inhibitor bortezomib represents an important advance in the treatment of multiple myeloma (MM). Bortezomib inhibits the activity of the 26S proteasome and induces cell death in a variety of tumor cells; however, the mechanism of cytotoxicity is not well understood. Methodology/Principal Findings: We investigated the differential phosphoproteome upon proteasome inhibition by using stable isotope labeling by amino acids in cell culture (SILAC) in combination with phosphoprotein enrichment and LC-MS/MS analysis. In total 233 phosphoproteins were identified and 72 phosphoproteins showed a 1.5-fold or greater change upon bortezomib treatment. The phosphoproteins with expression alterations encompass all major protein classes, including a large number of nucleic acid binding proteins. Site-specific phosphopeptide quantitation revealed that Ser38 phosphorylation on stathmin increased upon bortezomib treatment, suggesting new mechanisms associated to bortezomib-induced apoptosis in MM cells. Further studies demonstrated that stathmin phosphorylation profile was modified in response to bortezomib treatment and the regulation of stathmin by phosphorylation at specific Ser/Thr residues participated in the cellular response induced by bortezomib. Conclusions/Significance: Our systematic profiling of phosphorylation changes in response to bortezomib treatment not only advanced the global mechanistic understanding of the action of bortezomib on myeloma cells but also identified previously uncharacterized signaling proteins in myeloma cells.
英文摘要: Background: The proteasome inhibitor bortezomib represents an important advance in the treatment of multiple myeloma (MM). Bortezomib inhibits the activity of the 26S proteasome and induces cell death in a variety of tumor cells; however, the mechanism of cytotoxicity is not well understood.
关键词[WOS]: PROTEIN-KINASE CK2 ; MICROTUBULE DYNAMICS ; SIGNALING NETWORKS ; AMINO-ACIDS ; PHOSPHORYLATION ; AUTOPHAGY ; PS-341 ; PROTEOMICS ; APOPTOSIS ; BORTEZOMIB
语种: 英语
WOS记录号: WOS:000282269400031
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13743
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China
2.Jinan Univ, Natl Engn Res Ctr Genet Med, Guangzhou, Guangdong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
4.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China
5.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200030, Peoples R China

Recommended Citation:
Ge, Feng; Xiao, Chuan-Le; Bi, Li-Jun; Tao, Sheng-Ce; Xiong, Sheng; Yin, Xin-Feng; Li, Li-Ping; Lu, Chun-Hua; Jia, Hai-Tao; He, Qing-Yu.Quantitative Phosphoproteomics of Proteasome Inhibition in Multiple Myeloma Cells,PLOS ONE,2010,5(9):-
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