IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Quantitative Phosphoproteomics of Proteasome Inhibition in Multiple Myeloma Cells
Ge, Feng; Xiao, Chuan-Le; Bi, Li-Jun; Tao, Sheng-Ce; Xiong, Sheng; Yin, Xin-Feng; Li, Li-Ping; Lu, Chun-Hua; Jia, Hai-Tao; He, Qing-Yu; Ge, F, Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China
2010
Source PublicationPLOS ONE
ISSN1932-6203
Volume5Issue:9Pages:-
AbstractBackground: The proteasome inhibitor bortezomib represents an important advance in the treatment of multiple myeloma (MM). Bortezomib inhibits the activity of the 26S proteasome and induces cell death in a variety of tumor cells; however, the mechanism of cytotoxicity is not well understood. Methodology/Principal Findings: We investigated the differential phosphoproteome upon proteasome inhibition by using stable isotope labeling by amino acids in cell culture (SILAC) in combination with phosphoprotein enrichment and LC-MS/MS analysis. In total 233 phosphoproteins were identified and 72 phosphoproteins showed a 1.5-fold or greater change upon bortezomib treatment. The phosphoproteins with expression alterations encompass all major protein classes, including a large number of nucleic acid binding proteins. Site-specific phosphopeptide quantitation revealed that Ser38 phosphorylation on stathmin increased upon bortezomib treatment, suggesting new mechanisms associated to bortezomib-induced apoptosis in MM cells. Further studies demonstrated that stathmin phosphorylation profile was modified in response to bortezomib treatment and the regulation of stathmin by phosphorylation at specific Ser/Thr residues participated in the cellular response induced by bortezomib. Conclusions/Significance: Our systematic profiling of phosphorylation changes in response to bortezomib treatment not only advanced the global mechanistic understanding of the action of bortezomib on myeloma cells but also identified previously uncharacterized signaling proteins in myeloma cells.
KeywordProtein-kinase Ck2 Microtubule Dynamics Signaling Networks Amino-acids Phosphorylation Autophagy Ps-341 Proteomics Apoptosis Bortezomib
Department[Ge, Feng; Xiao, Chuan-Le; Xiong, Sheng; Yin, Xin-Feng; Li, Li-Ping; Lu, Chun-Hua; Jia, Hai-Tao; He, Qing-Yu] Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China; [Ge, Feng; Xiao, Chuan-Le; Xiong, Sheng; Yin, Xin-Feng; Li, Li-Ping; Lu, Chun-Hua; Jia, Hai-Tao; He, Qing-Yu] Jinan Univ, Natl Engn Res Ctr Genet Med, Guangzhou, Guangdong, Peoples R China; [Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Bi, Li-Jun] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China; [Tao, Sheng-Ce] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200030, Peoples R China
Subject AreaBiology ; Multidisciplinary Sciences
Funding OrganizationChang-Jiang Scholars Program ; ; Chang-Jiang Scholars Program ; ; Chang-Jiang Scholars Program ; ; Chang-Jiang Scholars Program ;
Indexed BySCI
Language英语
WOS IDWOS:000282269400031
Funding OrganizationChang-Jiang Scholars Program ; ; Chang-Jiang Scholars Program ; ; Chang-Jiang Scholars Program ; ; Chang-Jiang Scholars Program ;
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13743
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorGe, F, Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Ge, Feng,Xiao, Chuan-Le,Bi, Li-Jun,et al. Quantitative Phosphoproteomics of Proteasome Inhibition in Multiple Myeloma Cells[J]. PLOS ONE,2010,5(9):-.
APA Ge, Feng.,Xiao, Chuan-Le.,Bi, Li-Jun.,Tao, Sheng-Ce.,Xiong, Sheng.,...&Ge, F, Jinan Univ, Inst Life & Hlth Engn, Guangzhou, Guangdong, Peoples R China.(2010).Quantitative Phosphoproteomics of Proteasome Inhibition in Multiple Myeloma Cells.PLOS ONE,5(9),-.
MLA Ge, Feng,et al."Quantitative Phosphoproteomics of Proteasome Inhibition in Multiple Myeloma Cells".PLOS ONE 5.9(2010):-.
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