IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Identification of Novel 14-3-3 zeta Interacting Proteins by Quantitative Immunoprecipitation Combined with Knockdown (QUICK)
Ge, Feng; Li, Wen-Liang; Bi, Li-Jun; Tao, Sheng-Ce; Zhang, Zhi-Ping; Zhang, Xian-En; Ge, F, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2010
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume9Issue:11Pages:5848-5858
AbstractThe family of 14-3-3 proteins has emerged as critical regulators of diverse cellular responses under both physiological and pathological conditions. To gain insight into the molecular action of 14-3-3 zeta in multiple myeloma (MM), we performed a systematic proteomic analysis of 14-3-3 zeta-associated proteins. This analysis, recently developed by Matthias Mann, termed quantitative immunoprecipitation combined with knockdown (QUICK), integrates RNAi, SILAC, immunoprecipitation, and quantitative MS technologies. Quantitative mass spectrometry analysis allowed us to distinguish 14-3-3 zeta-interacting proteins from background proteins, resulting in the identification of 292 proteins in total with 95 novel interactions. Three 14-3-3 zeta-interacting proteins-BAX, HSP70, and BAG3-were further confirmed by reciprocal coimmunoprecipitations and colocalization analysis. Our results therefore not only uncover a large number of novel 14-3-3 zeta-associated proteins that possess a variety of cellular functions, but also provide new research directions for the study of the functions of 14-3-3 zeta. This study also demonstrated that QUICK is a useful approach to detect specific protein protein interactions with very high confidence and may have a wide range of applications in the investigation of protein complex interaction networks.
Keyword14-3-3 Zeta Quantitative Immunoprecipitation Combined With Knockdown (Quick) Multiple Myeloma (Mm) Bcl2-associated Athanogene 3 (Bag3) Bcl2-associated x Protein (Bax)
Department[Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Li, Wen-Liang] Tokai Univ, Sch Sci & Technol, Shibuya Ku, Tokyo 1510063, Japan; [Bi, Li-Jun] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China; [Tao, Sheng-Ce] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China; [Zhang, Zhi-Ping; Zhang, Xian-En] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
Subject AreaBiochemical Research Methods
Funding OrganizationChinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science
Indexed BySCI
Language英语
WOS IDWOS:000283810500033
Funding OrganizationChinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science ; Chinese Academy of Sciences ; National Protein Research Fund [2009CB825100, 2006CB910902]; Ministry of Health of China [2009ZX10004-107, 2008ZX10003-005]; State Key Development Program for Basic Research of China [2010CB529205]; Japan Society for the Promotion of Science
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13741
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorGe, F, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Ge, Feng,Li, Wen-Liang,Bi, Li-Jun,et al. Identification of Novel 14-3-3 zeta Interacting Proteins by Quantitative Immunoprecipitation Combined with Knockdown (QUICK)[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(11):5848-5858.
APA Ge, Feng.,Li, Wen-Liang.,Bi, Li-Jun.,Tao, Sheng-Ce.,Zhang, Zhi-Ping.,...&Ge, F, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.(2010).Identification of Novel 14-3-3 zeta Interacting Proteins by Quantitative Immunoprecipitation Combined with Knockdown (QUICK).JOURNAL OF PROTEOME RESEARCH,9(11),5848-5858.
MLA Ge, Feng,et al."Identification of Novel 14-3-3 zeta Interacting Proteins by Quantitative Immunoprecipitation Combined with Knockdown (QUICK)".JOURNAL OF PROTEOME RESEARCH 9.11(2010):5848-5858.
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