IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Zebrafish Ubc13 is required for Lys63-linked polyubiquitination and DNA damage tolerance
Li, Jie; Wen, Rui; Andersen, Parkeer; Liang, Yuping; Li, Qing; Xiao, Wei; Cui, Zongbin; Cui, ZB, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China
2010
Source PublicationMOLECULAR AND CELLULAR BIOCHEMISTRY
ISSN0300-8177
Volume343Issue:40180Pages:173-182
AbstractUbiquitination is an important post-translational protein modification that functions in diverse cellular processes of all eukaryotic organisms. Conventional Lys48-linked poly-ubiquitination leads to the degradation of specific proteins through 26S proteasomes, while Lys63-linked polyubiquitination appears to regulate protein activities in a non-proteolytic manner. To date, Ubc13 is the only known ubiquitin-conjugating enzyme capable of poly-ubiquitinating target proteins via Lys63-linked chains, and this activity absolutely requires a Ubc variant (Uev or Mms2) as a co-factor. However, Lys63-linked poly-ubiquitination and error-free DNA damage tolerance in zebrafish are yet to be defined. Here, we report molecular cloning and functional characterization of two zebrafish ubc13 genes, ubc13a and ubc13b. Analysis of their genomic structure, nucleotide and protein sequence indicates that the two genes are highly conserved during evolution and derived from whole genome duplication. Zebrafish Ubc13 proteins are able to physically interact with yeast or human Mms2 and both zebrafish ubc13 genes are able to functionally complement the yeast ubc13 null mutant for spontaneous mutagenesis and sensitivity to DNA damaging agents. In addition, upon DNA damage, the expression of zebrafish ubc13a and ubc13b is induced during embryogenesis and zebrafish Ubc13 is associated with nuclear chromatin. These results suggest the involvement of Lys63-linked poly-ubiquitylation in DNA damage response in zebrafish.
KeywordZebrafish Ubiquitin-conjugating Enzyme Lys63-linked Poly-ubiquitination Protein-protein Interaction Dna Damage Tolerance
Department[Li, Jie; Li, Qing; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Wen, Rui; Andersen, Parkeer; Liang, Yuping; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada; [Li, Jie] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Subject AreaCell Biology
Funding OrganizationNational Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240] ; National Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240] ; National Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240] ; National Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240]
Indexed BySCI
Language英语
WOS IDWOS:000282050300020
Funding OrganizationNational Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240] ; National Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240] ; National Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240] ; National Basic Research Program of China [2009CB941200]; Canadian Institutes of Health Research [MOP-53240]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13739
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCui, ZB, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Li, Jie,Wen, Rui,Andersen, Parkeer,et al. Zebrafish Ubc13 is required for Lys63-linked polyubiquitination and DNA damage tolerance[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2010,343(40180):173-182.
APA Li, Jie.,Wen, Rui.,Andersen, Parkeer.,Liang, Yuping.,Li, Qing.,...&Cui, ZB, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China.(2010).Zebrafish Ubc13 is required for Lys63-linked polyubiquitination and DNA damage tolerance.MOLECULAR AND CELLULAR BIOCHEMISTRY,343(40180),173-182.
MLA Li, Jie,et al."Zebrafish Ubc13 is required for Lys63-linked polyubiquitination and DNA damage tolerance".MOLECULAR AND CELLULAR BIOCHEMISTRY 343.40180(2010):173-182.
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