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Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis of zebrafish larvae
Yu, Liqin; Deng, Jun; Shi, Xiongjie; Liu, Chunsheng; Yu, Ke; Zhou, Bingsheng; Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2010
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume97Issue:3Pages:226-233
AbstractPolybrominated diphenyl ethers (PBDEs) have the potential to disrupt thyroid hormone homeostasis, but the molecular mechanisms underlying this process have not yet been clarified. In the present study, zebrafish (Danio rerio) embryos were exposed to a low concentration (0, 1, 3, and 10 mu g/L) of DE-71 from fertilization to 14 days thereafter. The whole-body content of thyroid hormone and transcription of genes in the hypothalamic-pituitary-thyroid (HPT) axis were analyzed. Exposure to up to 10 mu g/L of DE-71 significantly reduced thyroxine (T4) levels and significantly upregulated the transcription of corticotrophin-releasing hormone (CRH) and thyroid-stimulating hormone (TSH beta) genes in a concentration-dependent manner. The transcription of genes involved in the synthesis of TH proteins, sodium/iodide symporter (S1c5a5), and thyroglobulin (TG) and the transcription of marker genes associated with early thyroid development (Pax8 and Nkx2.1) were significantly upregulated upon DE-71 exposure. The expression of thyronine deiodinase (Deio1 and Deio2) mRNAs was also significantly upregulated, possibly as a compensatory response to the decreased T4 levels. However, DE-71 exposure resulted in the downregulation of transthyretin (1112) gene transcription and did not affect the transcription of thyroid hormone receptors (TRs). Exposure to DE-71 significantly induced the transcription of the uridinediphosphate-glucuronosyltransferase (UGT1ab) gene. The results of our study confirmed the reliability of the zebrafish larvae as models for assessment of the developmental toxicity of PBDEs and transcription of genes of the HPT axis can evaluate the potential mechanisms of thyroid disruption. (C) 2009 Elsevier B.V. All rights reserved.
KeywordPbdes Hypothalamic–pituitary–thyroid Axis Gene Expression Thyroid Hormone Zebrafish
Department[Yu, Liqin; Deng, Jun; Shi, Xiongjie; Liu, Chunsheng; Yu, Ke; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Yu, Liqin] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
Funding OrganizationMinistry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
Indexed BySCI
Language英语
WOS IDWOS:000277782100007
Funding OrganizationMinistry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; Ministry of Science and Technology of China [2006AA06Z420]; National Nature Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13709
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Yu, Liqin,Deng, Jun,Shi, Xiongjie,et al. Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis of zebrafish larvae[J]. AQUATIC TOXICOLOGY,2010,97(3):226-233.
APA Yu, Liqin.,Deng, Jun.,Shi, Xiongjie.,Liu, Chunsheng.,Yu, Ke.,...&Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2010).Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis of zebrafish larvae.AQUATIC TOXICOLOGY,97(3),226-233.
MLA Yu, Liqin,et al."Exposure to DE-71 alters thyroid hormone levels and gene transcription in the hypothalamic-pituitary-thyroid axis of zebrafish larvae".AQUATIC TOXICOLOGY 97.3(2010):226-233.
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