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The Role of Nrf2 and MAPK Pathways in PFOS-Induced Oxidative Stress in Zebrafish Embryos
Shi, Xiongjie; Zhou, Bingsheng; Zhou, BS, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Source PublicationTOXICOLOGICAL SCIENCES
ISSN1096-6080
Volume115Issue:2Pages:391-400
AbstractPerfluorooctane sulfonate (PFOS) is a persistent organic pollutant and causes oxidative stress, apoptosis, and developmental toxicity in zebrafish embryos. In the present study, we examined nuclear factor erythroid 2-related factor 2 (Nrf2)- and mitogen-activated protein kinases (MAPKs)-mediated oxidative stress pathways in zebrafish embryos upon exposure to PFOS. Four-hour postfertilization (hpf) zebrafish embryos were exposed to 0.2, 0.4, and 1.0 mg/l PFOS until 96 hpf. PFOS enhanced production of reactive oxygen species (ROS) in a concentration-dependent manner. Activity of antioxidative enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, was significantly induced in zebrafish larvae in all PFOS-treated groups relative to the control. Exposure to 1.0 mg/l PFOS significantly increased malondialdehyde production in zebrafish larvae. The Nrf2 and heme oxygenase-1 (HO-1) gene expressions were both significantly upregulated compared with the control group. For MAPKs, we investigated gene expression profiles of extracellular signal-regulated protein kinase (ERK), c-Jun NH (2)-terminal kinase (JNK), and p38. The ERK gene expression levels were unchanged, whereas JNK and p38 gene expressions were significantly upregulated, which could be linked to PFOS-induced cell apoptosis in zebrafish larvae. In addition, we found that coexposure with sulforaphane, an Nrf2 activator, could significantly protect against PFOS-induced ROS generation, whereas inhibition of MAPKs did not exhibit significant effects on PFOS-induced HO-1 gene expression and ROS production. Furthermore, we showed that morpholino-mediated knockdown of Nrf2 reduced PFOS-induced HO-1 gene expression. These findings demonstrate that Nrf2 is protective against PFOS-induced oxidative stress in zebrafish larvae.
KeywordPfos Ros Oxidative Stress Nrf2 Mapks Zebrafish
Department[Shi, Xiongjie; Zhou, Bingsheng] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaToxicology
Funding OrganizationNational Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
Indexed BySCI
Language英语
WOS IDWOS:000277997100009
Funding OrganizationNational Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] ; National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10]
Citation statistics
Cited Times:147[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13705
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Shi, Xiongjie,Zhou, Bingsheng,Zhou, BS, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. The Role of Nrf2 and MAPK Pathways in PFOS-Induced Oxidative Stress in Zebrafish Embryos[J]. TOXICOLOGICAL SCIENCES,2010,115(2):391-400.
APA Shi, Xiongjie,Zhou, Bingsheng,&Zhou, BS, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2010).The Role of Nrf2 and MAPK Pathways in PFOS-Induced Oxidative Stress in Zebrafish Embryos.TOXICOLOGICAL SCIENCES,115(2),391-400.
MLA Shi, Xiongjie,et al."The Role of Nrf2 and MAPK Pathways in PFOS-Induced Oxidative Stress in Zebrafish Embryos".TOXICOLOGICAL SCIENCES 115.2(2010):391-400.
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