IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209)
Jin, Shiwei; Yang, Fangxing; Hui, Yang; Xu, Ying; Lu, Yuanan; Liu, Jiankang; Xu, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Source PublicationTOXICOLOGY IN VITRO
ISSN0887-2333
Volume24Issue:4Pages:1190-1196
AbstractRecently, the environmental residues of polybrominated diphenyl ethers (PBDEs) have markedly increased. In particular, the levels of certain PBDE congeners in fish have raised concern regarding potential risks associated with dietary PBDEs exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro fish cell models. In this study, the cytotoxic effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209) on RTG-2 cells were investigated. RTG-2 cells were incubated with different concentrations of BDE-47 and BDE-209 (1-100 mu M) for 72 h, and a set of bioassays were conducted to measure: cell viability (evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and neutral red (NR) uptake), lactate dehydrogenase (LDH) leakage, reactive oxygen species (ROS) formation and cell apoptosis. The results showed that BDE-47 and BDE-209 inhibited the cells viability, increased LDH leakage, and induced cell apoptosis in time and concentration-dependent manner. All significant effects were observed at concentrations of 12.5 mu M and above for BDE-47 and 25 mu M and above for BDE-209 (P < 0.05). At the concentration of 100 mu M BDE-47 and BDE-209, the cell viability of the exposed cells dropped to about 40% and 50% of the control, and the apoptotic rates were 52.6% and 34.6%, respectively. After 12 h exposure, a concentration-dependent increases of BDE-47 and BDE-209 (12.5-100 mu M) in ROS formation were observed. Collectively, the results of cell viability. LDH leakage, cell apoptosis and ROS formation demonstrated that the toxic mechanism of PBDEs on RTG-2 might be mediated by oxidative stress. (C) 2010 Elsevier Ltd. All rights reserved.
Keyword2,2 ',4,4 '-tetrabromodiphenyl Ether Decabrominated Diphenyl Ether Rtg-2 Cells Cytotoxicity Apoptosis Reactive Oxygen Species
Department[Jin, Shiwei; Yang, Fangxing; Hui, Yang; Xu, Ying; Lu, Yuanan; Liu, Jiankang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Jin, Shiwei] Wuhan Inst Technol, Key Lab Green Chem Proc, Minist Educ, Wuhan 430073, Peoples R China; [Lu, Yuanan] Univ Hawaii, Dept Publ Hlth Sci, John A Burns Sch Med, Honolulu, HI 96822 USA
Subject AreaToxicology
Funding OrganizationNational Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] ; National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] ; National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] ; National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424]
Indexed BySCI
Language英语
WOS IDWOS:000279124900019
Funding OrganizationNational Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] ; National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] ; National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424] ; National Nature Science Foundation of China [20890113, 20607030]; Wuhan Bureau of Science and Technology [200750731259]; Hi-Tech Research and Development Program of China [2006AA06Z424]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13693
Collection水环境工程研究中心_期刊论文
Corresponding AuthorXu, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jin, Shiwei,Yang, Fangxing,Hui, Yang,et al. Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209)[J]. TOXICOLOGY IN VITRO,2010,24(4):1190-1196.
APA Jin, Shiwei.,Yang, Fangxing.,Hui, Yang.,Xu, Ying.,Lu, Yuanan.,...&Xu, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2010).Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209).TOXICOLOGY IN VITRO,24(4),1190-1196.
MLA Jin, Shiwei,et al."Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209)".TOXICOLOGY IN VITRO 24.4(2010):1190-1196.
Files in This Item:
File Name/Size DocType Version Access License
Cytotoxicity and apo(618KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jin, Shiwei]'s Articles
[Yang, Fangxing]'s Articles
[Hui, Yang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jin, Shiwei]'s Articles
[Yang, Fangxing]'s Articles
[Hui, Yang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jin, Shiwei]'s Articles
[Yang, Fangxing]'s Articles
[Hui, Yang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Cytotoxicity and apoptosis induction on RTG-2 cells of 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) and decabrominated diphenyl ether (BDE-209).pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.