中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水环境工程研究中心  > 期刊论文
学科主题: Chemistry, Analytical
题名: A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater
作者: Tu, Xinhai2; Xiao, Bangding1; Xiong, Jian2; Chen, Xudong
通讯作者: Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China
关键词: Nitrate ; Nitrite ; Microplate ; Water analysis
刊名: TALANTA
发表日期: 2010-08-15
DOI: 10.1016/j.talanta.2010.06.002
卷: 82, 期:3, 页:976-983
收录类别: SCI
文章类型: Article
部门归属: [Xiao, Bangding] Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, Wuhan 430072, Hubei, Peoples R China; [Tu, Xinhai; Xiong, Jian] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
资助者: Special Program Water Pollution Control in China [2008ZX07102-005]; Science Foundation of NSFC-Yunnan Province [U0833604]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
摘要: A rapid, simple miniaturised photometrical method was developed for the determination of nitrate and/or nitrite in freshwater samples. All procedures, including sample buffering, reduction by copperised cadmium granules, colour development and absorbance determination, were completed in a 96-well microplate. The factors governing the nitrate reduction and its recovery were investigated in detail, and the optimised analysing conditions were established. Nitrate was quantitatively reduced by copperised cadmium granules with a high reduction efficiency (96.59 +/- 0.96%). The proposed method gave a linear calibration ranging from 0.01 to 1.50 mg L-1 for NO2--N and 0.02 to 1.50 mg L-1 for NO3--N. The detection limits for nitrite and nitrate were 2 and 4 mu g L-1, respectively. The proposed method allowed at least 48 samples to be simultaneously analysed in duplicate, with good precision, within 90 min for nitrate and 30 min for nitrite, and was successfully applied to actual freshwater sample analysis with a recovery of 98.02 +/- 1.04% for nitrite and 99.72 +/- 1.39% for nitrate. This method produced accurate results comparable to standard methods, provided a much higher sample throughput than conventional methods and could be routinely used in actual freshwater sample monitoring. (C) 2010 Elsevier B.V. All rights reserved.
英文摘要: A rapid, simple miniaturised photometrical method was developed for the determination of nitrate and/or nitrite in freshwater samples. All procedures, including sample buffering, reduction by copperised cadmium granules, colour development and absorbance determination, were completed in a 96-well microplate. The factors governing the nitrate reduction and its recovery were investigated in detail, and the optimised analysing conditions were established. Nitrate was quantitatively reduced by copperised cadmium granules with a high reduction efficiency (96.59 +/- 0.96%). The proposed method gave a linear calibration ranging from 0.01 to 1.50 mg L(-1) for NO(2)(-)-N and 0.02 to 1.50 mg L(-1) for NO(3)(-)-N. The detection limits for nitrite and nitrate were 2 and 4 mu g L(-1), respectively. The proposed method allowed at least 48 samples to be simultaneously analysed in duplicate, with good precision, within 90 min for nitrate and 30 min for nitrite, and was successfully applied to actual freshwater sample analysis with a recovery of 98.02 +/- 1.04% for nitrite and 99.72 +/- 1.39% for nitrate. This method produced accurate results comparable to standard methods, provided a much higher sample throughput than conventional methods and could be routinely used in actual freshwater sample monitoring. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: FLOW-INJECTION ANALYSIS ; SPECTROPHOTOMETRIC DETERMINATION ; CADMIUM COLUMN ; COPPER-CADMIUM ; REDUCTION ; PHOSPHATE ; SYSTEM ; SOIL
语种: 英语
WOS记录号: WOS:000281177100017
ISSN号: 0039-9140
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13687
Appears in Collections:水环境工程研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater.pdf(335KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Lab Chem Ecol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Tu, Xinhai; Xiao, Bangding; Xiong, Jian; Chen, Xudong.A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater,TALANTA,2010,82(3):976-983
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Tu, Xinhai]'s Articles
[Xiao, Bangding]'s Articles
[Xiong, Jian]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Tu, Xinhai]‘s Articles
[Xiao, Bangding]‘s Articles
[Xiong, Jian]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: A simple miniaturised photometrical method for rapid determination of nitrate and nitrite in freshwater.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace