IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii
Xu, Qiaoqing; Wang, Gailing; Yuan, Hanwen; Chai, Yi; Xiao, Zhili; Xu, QQ, Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China
2010
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume157Issue:1Pages:119-126
AbstractBivalve molluscs rely on the interaction between cellular and humoral factors for protection against potential pathogens. Antimicrobial peptides (AMPS) have been proven to be one of the most important humoral components that afford resistance to pathogen infection. The AMP gene to be identified was that encoding theromacin in the triangle-shell pearl mussel Hyriopsis cumingii (Hc theromacin); this gene was identified from a suppression subtractive hybridization library, and subsequently cloned by 3 ' and 5 ' rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). The full-length theromacin cDNA contains 547 bp, with a 294-bp open reading frame that encodes a 97-amino acid peptide, and the deduced peptide sequence contains a 61-amino acid putative mature peptide. The sequence also contains 10 cysteine residues. Reverse transcriptase (RT)-PCR analysis showed that Hc theromacin transcripts were constitutively expressed in the liver, foot, gill, adductor muscle, heart, mantle, intestine, and hemocytes, with the highest level in hemocytes. Theromacin mRNA levels were found to increase after challenge with Gram-positive and Gram-negative bacteria. After injection of the Gram-positive bacteria Staphylococcus aureus and Bifidobacterium bifidum, Hc theromacin expression showed the highest fold-change at 48 and 36 h after infection, respectively, and its levels decreased gradually thereafter. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
KeywordAntibacterial Peptide Theromacin Mussel Hemocyte Gram-positive Bacteria
Department[Xu, Qiaoqing; Yuan, Hanwen; Chai, Yi; Xiao, Zhili] Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China; [Wang, Gailing] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Zoology
Funding OrganizationNatural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] ; Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] ; Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] ; Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204]
Indexed BySCI
Language英语
WOS IDWOS:000280027300016
Funding OrganizationNatural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] ; Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] ; Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204] ; Natural Science Foundation of Hubei province, China [2008CDB104]; Eduational Research Program of Hubei, China [Q20081204]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13663
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorXu, QQ, Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Xu, Qiaoqing,Wang, Gailing,Yuan, Hanwen,et al. cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,157(1):119-126.
APA Xu, Qiaoqing,Wang, Gailing,Yuan, Hanwen,Chai, Yi,Xiao, Zhili,&Xu, QQ, Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China.(2010).cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,157(1),119-126.
MLA Xu, Qiaoqing,et al."cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 157.1(2010):119-126.
Files in This Item:
File Name/Size DocType Version Access License
cDNA sequence and ex(766KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Qiaoqing]'s Articles
[Wang, Gailing]'s Articles
[Yuan, Hanwen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Qiaoqing]'s Articles
[Wang, Gailing]'s Articles
[Yuan, Hanwen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Qiaoqing]'s Articles
[Wang, Gailing]'s Articles
[Yuan, Hanwen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: cDNA sequence and expression analysis of an antimicrobial peptide, theromacin, in the triangle-shell pearl mussel Hyriopsis cumingii.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.