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学科主题: Immunology; Zoology
题名: Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection
作者: Zhou, Xiuxia1, 2; Feng, Hong1, 2; Guo, Qionglin1; Dai, Heping1
通讯作者: Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: CD9 ; Tetraspanin family ; cDNA cloning ; Bacterial infection ; Chinese soft-shelled turtle
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2010-02-01
DOI: 10.1016/j.dci.2009.09.001
卷: 34, 期:2, 页:150-157
收录类别: SCI
文章类型: Article
部门归属: [Zhou, Xiuxia; Feng, Hong; Guo, Qionglin; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Xiuxia; Feng, Hong] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704]
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
摘要: In this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first time. The full-length cDNA of turtle CD9 was 1146 bp and contained a 672 bp open reading frame (ORF) coding for a protein of 224 amino acids. Four transmembrane domains (TMs) divided the turtle CD9 into several parts: short N-, C-termini, an intracellular loop and two (small and large) extracellular loops (SEL and LEL). A CCG motif. a potential N-linked glycosylation site and 10 cysteine residues were well conserved. The deduced amino acid sequence analysis showed that the turtle CD9 shared 82% identity to duck CD9. Most of the differences were found in the LEL Phylogenetic analysis showed that the turtle CD9 sequence clustered together with bird CD9 sequence. RT-PCR analysis showed that turtle CD9 was ubiquitously expressed in liver, spleen, kidney, heart, blood and intestine tissues of un-infected turtles. Real-time PCR analysis further indicated that after Aeromonas hydrophila infection, the turtle CD9 mRNA was up-regulated in various tissues at 8 h, and significantly up-regulated during 8 h to 7 d. These results indicated that turtle CD9 may be involved in anti-bacterial immune response. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要: In this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first time. The full-length cDNA of turtle CD9 was 1146 bp and contained a 672 bp open reading frame (ORF) coding for a protein of 224 amino acids. Four transmembrane domains (TMs) divided the turtle CD9 into several parts: short N-, C-termini, an intracellular loop and two (small and large) extracellular loops (SEL and LEL). A CCG motif. a potential N-linked glycosylation site and 10 cysteine residues were well conserved. The deduced amino acid sequence analysis showed that the turtle CD9 shared 82% identity to duck CD9. Most of the differences were found in the LEL Phylogenetic analysis showed that the turtle CD9 sequence clustered together with bird CD9 sequence. RT-PCR analysis showed that turtle CD9 was ubiquitously expressed in liver, spleen, kidney, heart, blood and intestine tissues of un-infected turtles. Real-time PCR analysis further indicated that after Aeromonas hydrophila infection, the turtle CD9 mRNA was up-regulated in various tissues at 8 h, and significantly up-regulated during 8 h to 7 d. These results indicated that turtle CD9 may be involved in anti-bacterial immune response. (C) 2009 Elsevier Ltd. All rights reserved.
关键词[WOS]: CELL-SURFACE ; MOLECULAR-CLONING ; TETRASPANIN CD9 ; EXTRACELLULAR DOMAIN ; PROTEINS ; ANTIGEN ; INTEGRINS ; SUPERFAMILY ; ACTIVATION ; ADHESION
语种: 英语
WOS记录号: WOS:000273147400009
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13661
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Zhou, Xiuxia; Feng, Hong; Guo, Qionglin; Dai, Heping.Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(2):150-157
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