IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection
Zhou, Xiuxia; Feng, Hong; Guo, Qionglin; Dai, Heping; Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume34Issue:2Pages:150-157
AbstractIn this study, a CD9 homologue in a reptile, Chinese soft-shelled turtle, has been cloned and identified for the first time. The full-length cDNA of turtle CD9 was 1146 bp and contained a 672 bp open reading frame (ORF) coding for a protein of 224 amino acids. Four transmembrane domains (TMs) divided the turtle CD9 into several parts: short N-, C-termini, an intracellular loop and two (small and large) extracellular loops (SEL and LEL). A CCG motif. a potential N-linked glycosylation site and 10 cysteine residues were well conserved. The deduced amino acid sequence analysis showed that the turtle CD9 shared 82% identity to duck CD9. Most of the differences were found in the LEL Phylogenetic analysis showed that the turtle CD9 sequence clustered together with bird CD9 sequence. RT-PCR analysis showed that turtle CD9 was ubiquitously expressed in liver, spleen, kidney, heart, blood and intestine tissues of un-infected turtles. Real-time PCR analysis further indicated that after Aeromonas hydrophila infection, the turtle CD9 mRNA was up-regulated in various tissues at 8 h, and significantly up-regulated during 8 h to 7 d. These results indicated that turtle CD9 may be involved in anti-bacterial immune response. (C) 2009 Elsevier Ltd. All rights reserved.
KeywordCd9 Tetraspanin Family Cdna Cloning Bacterial Infection Chinese Soft-shelled Turtle
Department[Zhou, Xiuxia; Feng, Hong; Guo, Qionglin; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhou, Xiuxia; Feng, Hong] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaImmunology ; Zoology
Funding OrganizationNational Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704]
Indexed BySCI
Language英语
WOS IDWOS:000273147400009
Funding OrganizationNational Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13661
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Xiuxia,Feng, Hong,Guo, Qionglin,et al. Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(2):150-157.
APA Zhou, Xiuxia,Feng, Hong,Guo, Qionglin,Dai, Heping,&Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2010).Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(2),150-157.
MLA Zhou, Xiuxia,et al."Identification and characterization of the first reptilian CD9, and its expression analysis in response to bacterial infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.2(2010):150-157.
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