IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization
Shi, Yan; Zhao, Zhe; Yin, Jing-Kui; Zhu, Xin-Ping; Chen, Kun-Ci; Pan, De-Bo; Gui, Jian-Fang; Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
2010
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume155Issue:2Pages:110-117
AbstractInterferon regulatory factor 2 (IRF-2) is a multifunctional transcription factor which exhibits both transcriptional activating and repressing activities in the IRF family. In this study, we report an IRF-2 gene isolated from orange-spotted grouper (Epinephelus coioides). The 1854 bp full-length cDNA sequence of EcIRF-2 has been cloned, encoding a putative peptide of 336 amino acids which is highly consistent with the feature of IRF family members. The genomic fragment of EcIRF-2 contains nine exons and eight introns, spanning over approximate 8.8 kb. The expression of EcIRF-2 gene was detected in various tissues of healthy orange-spotted grouper and in four tissues after being challenged with poly I:C or LPS. EcIRF-2 gene is ubiquitously expressed in various healthy fish tissues and is up-regulated in vivo in response to poly I:C or LPS. Subcellular localization analysis of EcIRF-2 suggests it is an intranuclear protein in the fish cells. We believe this research is the first report of fish IRF-2 protein localization. The results in this research establish the base for further study of function mechanism of IRF family members in orange-spotted grouper. (C) 2009 Elsevier Inc. All rights reserved.
KeywordInterferon Regulator Factor-2 (Irf-2) Epinephelus Coioides Genomic Structure Inductive Expression Subcellular Localization
Department[Shi, Yan; Yin, Jing-Kui; Zhu, Xin-Ping; Chen, Kun-Ci; Pan, De-Bo] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Zhao, Zhe] Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhu, Xin-Ping] Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China; [Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Zoology
Funding OrganizationNational Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007]
Indexed BySCI
Language英语
WOS IDWOS:000273927000003
Funding OrganizationNational Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007, 30901117]; fund of the Key Laboratory of Fisheries Genetic Resources & Aquaculture, Chinese Academy of Fisheries Sciences [2008A007]
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13655
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Shi, Yan,Zhao, Zhe,Yin, Jing-Kui,et al. Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2010,155(2):110-117.
APA Shi, Yan.,Zhao, Zhe.,Yin, Jing-Kui.,Zhu, Xin-Ping.,Chen, Kun-Ci.,...&Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China.(2010).Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,155(2),110-117.
MLA Shi, Yan,et al."Interferon regulatory factor-2 in orange-spotted grouper (Epinephelus coioides): Gene, inductive expression pattern and subcellular localization".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 155.2(2010):110-117.
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