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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella
作者: Chen, W. Q.1, 2, 3; Xu, Q. Q.1, 3, 4; Chang, M. X.1; Nie, P.1, 3; Peng, K. M.2
通讯作者: Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
关键词: Grass carp ; NOD1 ; NOD2 ; Grass carp reovirus
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2010
DOI: 10.1016/j.fsi.2009.09.012
卷: 28, 期:1, 页:18-29
收录类别: SCI
文章类型: Article
部门归属: [Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Chen, W. Q.; Peng, K. M.] Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China; [Chen, W. Q.; Xu, Q. Q.; Nie, P.] Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Xu, Q. Q.] Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30830083]
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Nuclear oligomerization domains (NODS) are cytosolic pattern recognition receptors (PRRs) to detect bacterial component. In this study, the molecular cloning and genomic characterization of grass carp NODI (gcNOD1) and grass carp NOD2 (gcNOD2) were reported. The complete open reading frame of gcNOD1 contains 2814 bp, encoding a 937-amino acid polypeptide. The gcNCD2 cDNA sequence encodes 982-amino acid polypeptide. Both gcNOD1 and gcNOD2 possess three conserved domains: carboxy terminal leucine rich repeat (LRR) domains, a central NOD, NBS or NACHT domain, and an amino terminal CARD domain (two in the case of NOD2). At the genomic level, gcNOD1 consists of 11 exons, with 10 intervening introns, spanning approximately 9 kb of genomic sequence. Whereas gcNOD2 has a length of approximately 5 kb with 9 intervening introns. Real time PCR analysis showed gcNOD1 and gcNOD2 were ubiquitously expressed in adult tissues. The highest transcript level of gcNOD1 was detected in brain, but in head kidney for gcNOD2. Grass carp reovirus significantly induced the expression of gcNOD1 and gcNOD2 in spleen (from days 1 to 6). However, expression profiles differed in time course response. Induction experiments with lipopolysaccharide (LPS), peptidoglycan (PGN) and polyI:C revealed the differential expression and regulation of gcNOD1 and gcNOD2 in blood, head kidney, trunk kidney and spleen. All these data suggest a potential role of NOD1 and NOD2 in fish innate immune protection to bacterial and virus infections. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要: Nuclear oligomerization domains (NODS) are cytosolic pattern recognition receptors (PRRs) to detect bacterial component. In this study, the molecular cloning and genomic characterization of grass carp NODI (gcNOD1) and grass carp NOD2 (gcNOD2) were reported. The complete open reading frame of gcNOD1 contains 2814 bp, encoding a 937-amino acid polypeptide. The gcNCD2 cDNA sequence encodes 982-amino acid polypeptide. Both gcNOD1 and gcNOD2 possess three conserved domains: carboxy terminal leucine rich repeat (LRR) domains, a central NOD, NBS or NACHT domain, and an amino terminal CARD domain (two in the case of NOD2). At the genomic level, gcNOD1 consists of 11 exons, with 10 intervening introns, spanning approximately 9 kb of genomic sequence. Whereas gcNOD2 has a length of approximately 5 kb with 9 intervening introns. Real time PCR analysis showed gcNOD1 and gcNOD2 were ubiquitously expressed in adult tissues. The highest transcript level of gcNOD1 was detected in brain, but in head kidney for gcNOD2. Grass carp reovirus significantly induced the expression of gcNOD1 and gcNOD2 in spleen (from days 1 to 6). However, expression profiles differed in time course response. Induction experiments with lipopolysaccharide (LPS), peptidoglycan (PGN) and polyI:C revealed the differential expression and regulation of gcNOD1 and gcNOD2 in blood, head kidney, trunk kidney and spleen. All these data suggest a potential role of NOD1 and NOD2 in fish innate immune protection to bacterial and virus infections. (C) 2009 Elsevier Ltd. All rights reserved.
关键词[WOS]: NF-KAPPA-B ; FUNCTIONAL-CHARACTERIZATION ; RIG-I ; DIAMINOPIMELIC ACID ; INTERFERON-GAMMA ; FAMILY-MEMBER ; RNA HELICASE ; GENE ; LIPOPOLYSACCHARIDE ; RECOGNITION
语种: 英语
WOS记录号: WOS:000274235100003
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13653
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China
3.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
4.Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China

Recommended Citation:
Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.; Peng, K. M..Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella,FISH & SHELLFISH IMMUNOLOGY,2010,28(1):18-29
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