| Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella | |
| Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.; Peng, K. M.; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China | |
| 2010 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 28Issue:1Pages:18-29 |
| Abstract | Nuclear oligomerization domains (NODS) are cytosolic pattern recognition receptors (PRRs) to detect bacterial component. In this study, the molecular cloning and genomic characterization of grass carp NODI (gcNOD1) and grass carp NOD2 (gcNOD2) were reported. The complete open reading frame of gcNOD1 contains 2814 bp, encoding a 937-amino acid polypeptide. The gcNCD2 cDNA sequence encodes 982-amino acid polypeptide. Both gcNOD1 and gcNOD2 possess three conserved domains: carboxy terminal leucine rich repeat (LRR) domains, a central NOD, NBS or NACHT domain, and an amino terminal CARD domain (two in the case of NOD2). At the genomic level, gcNOD1 consists of 11 exons, with 10 intervening introns, spanning approximately 9 kb of genomic sequence. Whereas gcNOD2 has a length of approximately 5 kb with 9 intervening introns. Real time PCR analysis showed gcNOD1 and gcNOD2 were ubiquitously expressed in adult tissues. The highest transcript level of gcNOD1 was detected in brain, but in head kidney for gcNOD2. Grass carp reovirus significantly induced the expression of gcNOD1 and gcNOD2 in spleen (from days 1 to 6). However, expression profiles differed in time course response. Induction experiments with lipopolysaccharide (LPS), peptidoglycan (PGN) and polyI:C revealed the differential expression and regulation of gcNOD1 and gcNOD2 in blood, head kidney, trunk kidney and spleen. All these data suggest a potential role of NOD1 and NOD2 in fish innate immune protection to bacterial and virus infections. (C) 2009 Elsevier Ltd. All rights reserved. |
| Keyword | Grass Carp Nod1 Nod2 Grass Carp Reovirus |
| Department | [Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Chen, W. Q.; Peng, K. M.] Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China; [Chen, W. Q.; Xu, Q. Q.; Nie, P.] Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Xu, Q. Q.] Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China |
| Subject Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| Funding Organization | National Natural Science Foundation of China [30830083] ; National Natural Science Foundation of China [30830083] ; National Natural Science Foundation of China [30830083] ; National Natural Science Foundation of China [30830083] |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000274235100003 |
| Funding Organization | National Natural Science Foundation of China [30830083] ; National Natural Science Foundation of China [30830083] ; National Natural Science Foundation of China [30830083] ; National Natural Science Foundation of China [30830083] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/13653 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, W. Q.,Xu, Q. Q.,Chang, M. X.,et al. Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(1):18-29. |
| APA | Chen, W. Q.,Xu, Q. Q.,Chang, M. X.,Nie, P.,Peng, K. M.,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.(2010).Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella.FISH & SHELLFISH IMMUNOLOGY,28(1),18-29. |
| MLA | Chen, W. Q.,et al."Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella".FISH & SHELLFISH IMMUNOLOGY 28.1(2010):18-29. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Molecular characteri（4445KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment