IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)
Shi, Yan; Zhu, Xin-Ping; Yin, Jing-Kui; Zhang, Qi-Ya; Gui, Jian-Fang; Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
2010
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume37Issue:3Pages:1483-1493
AbstractInterferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5'-terminal untranslated region (5'-UTR) of 153 bp, and a 3'-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5'-UTR sequence contains an exon and an intron, and the 3'-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.
KeywordInterferon Regulator Factor-1 (Irf-1) Epinephelus Coioides Genomic Structure Inductive Expression Subcellular Localization
Department[Shi, Yan; Zhu, Xin-Ping; Yin, Jing-Kui] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Zhang, Qi-Ya; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhu, Xin-Ping] Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007]
Indexed BySCI
Language英语
WOS IDWOS:000274212500043
Funding OrganizationNational Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007] ; National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13651
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Shi, Yan,Zhu, Xin-Ping,Yin, Jing-Kui,et al. Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)[J]. MOLECULAR BIOLOGY REPORTS,2010,37(3):1483-1493.
APA Shi, Yan,Zhu, Xin-Ping,Yin, Jing-Kui,Zhang, Qi-Ya,Gui, Jian-Fang,&Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China.(2010).Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides).MOLECULAR BIOLOGY REPORTS,37(3),1483-1493.
MLA Shi, Yan,et al."Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)".MOLECULAR BIOLOGY REPORTS 37.3(2010):1483-1493.
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