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学科主题: Biochemistry & Molecular Biology
题名: Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)
作者: Shi, Yan1; Zhu, Xin-Ping1, 3; Yin, Jing-Kui1; Zhang, Qi-Ya2; Gui, Jian-Fang2
通讯作者: Zhu, XP, Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
关键词: Interferon regulator factor-1 (IRF-1) ; Epinephelus coioides ; Genomic structure ; Inductive expression ; Subcellular localization
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2010-03-01
DOI: 10.1007/s11033-009-9544-0
卷: 37, 期:3, 页:1483-1493
收录类别: SCI
文章类型: Article
部门归属: [Shi, Yan; Zhu, Xin-Ping; Yin, Jing-Kui] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Zhang, Qi-Ya; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhu, Xin-Ping] Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China [2004CB117401]; National Natural Science Foundation of China [U0631007]
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5'-terminal untranslated region (5'-UTR) of 153 bp, and a 3'-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5'-UTR sequence contains an exon and an intron, and the 3'-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.
英文摘要: Interferon-regulatory factor 1 (IRF-1) is the first member of IRF family, which is involved in many biological processes such as immune response, antiviral defense, cell growth regulation, and apoptosis. In this study, an IRF-1 gene, EcIRF-1, was isolated and characterized from orange-spotted grouper (Epinephelus coioides). The full-length cDNA of EcIRF-1 is 1,730 bp, including an open reading frame of 906 bp, a 5'-terminal untranslated region (5'-UTR) of 153 bp, and a 3'-UTR of 671 bp. The EcIRF-1 gene consists of 10 exons and 9 introns, spanning over approximate 4.3 kb of genomic sequence. The 5'-UTR sequence contains an exon and an intron, and the 3'-UTR sequence is included in the last exon. Expression analysis by real-time PCR reveals that the EcIRF-1 gene is ubiquitously expressed in various healthy fish tissues, whereas its expression is upregulated in vivo in response to polyinosinic-polycytidylic acid or lipopolysaccharide stimulation. Subcellular localization analysis shows the EcIRF-1 is an intranuclearly localized and immobile protein in the cultured fish cells. Data presented in this paper provide an important base to further understand EcIRF-1 gene function and its regulation associated with interferon immune system in orange-spotted grouper.
关键词[WOS]: CARASSIUS-AURATUS L. ; UV-INACTIVATED VIRUS ; IFN-INDUCIBLE GENES ; MOLECULAR-CLONING ; PARALICHTHYS-OLIVACEUS ; EXPRESSION ANALYSIS ; SUBCELLULAR-LOCALIZATION ; TRANSCRIPTION FACTOR ; JAPANESE FLOUNDER ; FISH CELLS
语种: 英语
WOS记录号: WOS:000274212500043
ISSN号: 0301-4851
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13651
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Guangzhou 510380, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Guangzhou Univ, Coll Life Sci, Guangzhou 510006, Guangdong, Peoples R China

Recommended Citation:
Shi, Yan; Zhu, Xin-Ping; Yin, Jing-Kui; Zhang, Qi-Ya; Gui, Jian-Fang.Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides),MOLECULAR BIOLOGY REPORTS,2010,37(3):1483-1493
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