IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection
Xiao, Zhan-Gang; Liu, Hong; Fu, Jian-Ping; Hu, Wei; Wang, Ya-Ping; Guo, Qiong-Lin; Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume28Issue:2Pages:362-371
AbstractAs a member of a newly discovered protein family, the suppressor of cytokine signalling 3 (SOCS-3) has been shown to regulate the responses of many immune cytokines in a negative auto-regulatory manner. The full-length cDNA of common carp SOCS-3 was 1603 bp and contained a 630 bp open reading frame (ORF) coding for a protein of 209 amino acids. Carp SOCS-3 molecule was well conserved especially in the SRC homology 2 (SH2) and the SOCS box. The kinase inhibitory region (KIR) and ESS domains, upstream of the SH2 domain, were conserved in carp SOCS-3, except for a specific insertion (PHRYK) in the KIR domain at the N-terminal region. Three conserved cysteine (Cys-102, 124 and 193) residues, and one additional cysteine (Cys-168) residue, were also found in carp SOCS-3. The 2015 bp genomic DNA of carp SOCS-3 contained two exons and one intron. Phylogenetic analysis showed that carp SOCS-3 sequence grouped with other known fish SOCS-3 sequences with zebrafish SOCS-3 as the closest neighbour. RT-PCR analysis showed that carp SOCS-3 was initially expressed at 4 h pf (post-fertilization) and gradually increased up to 4 w pf during embryogenesis. By RT-qPCR analysis, carp SOCS-3 gene was predominantly detected in gill, head kidney, thymus and skin, followed by spleen and peripheral blood, lower expression level was detected in kidney, intestine, liver and muscle; the SOCS-3 transcript was significantly increased in thymus, head kidney, spleen and intestine of GH (growth hormone)-transgenic carp; after SVCV (spring viraemia of carp virus) infection, the carp SOCS-3 transcript was significantly up-regulated in gill, intestine, thymus, spleen, head kidney and kidney tissues in a time-dependent manner. These results suggest that teleost SOCS-3 may play an active role in the modulation of viral-induced innate immune response and in preventing the overaction of some cytokines with viral stimulation. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
KeywordSocs-3 Common Carp Embryogenesis Gh-transgenic Carp Spring Viraemia Of Carp Virus (Svcv)
Department[Xiao, Zhan-Gang; Fu, Jian-Ping; Hu, Wei; Wang, Ya-Ping; Guo, Qiong-Lin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, Hong] Shenzhen Exit & Entry Inspect & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen 518001, Peoples R China; [Xiao, Zhan-Gang; Fu, Jian-Ping] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]
Indexed BySCI
Language英语
WOS IDWOS:000274721300015
Funding OrganizationNational Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704] ; National Natural Science Foundation of China [30671599, 30871912]; National Basic Research Program of China [2009CB118704]
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Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13647
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Zhan-Gang,Liu, Hong,Fu, Jian-Ping,et al. Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):362-371.
APA Xiao, Zhan-Gang.,Liu, Hong.,Fu, Jian-Ping.,Hu, Wei.,Wang, Ya-Ping.,...&Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2010).Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection.FISH & SHELLFISH IMMUNOLOGY,28(2),362-371.
MLA Xiao, Zhan-Gang,et al."Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):362-371.
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