IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos
Hong, Ni; Li, Mingyou; Zeng, Zhiqiang; Yi, Meisheng; Deng, Jiaorong; Gui, Jianfang; Winkler, Christoph; Schartl, Manfred; Hong, Yunhan; Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
2010
Source PublicationCELLULAR AND MOLECULAR LIFE SCIENCES
ISSN1420-682X
Volume67Issue:7Pages:1189-1202
AbstractChimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i (1) , i (3) and af), only strain i (1) generated pigmented chimeras. Strikingly, this accessibility is completely lost in i (1) but acquired in i (3) after host gamma-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production.
KeywordChimera Es Cell Host Accessibility Gamma-irradiation Medaka
Department[Hong, Ni; Li, Mingyou; Zeng, Zhiqiang; Yi, Meisheng; Deng, Jiaorong; Winkler, Christoph; Hong, Yunhan] Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore; [Gui, Jianfang; Hong, Yunhan] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China; [Schartl, Manfred] Univ Wurzburg, Bioctr, D-97074 Wurzburg, Germany
Subject AreaBiochemistry & Molecular Biology ; Cell Biology
Funding OrganizationBiomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union ; Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union ; Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union ; Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union
Indexed BySCI
Language英语
WOS IDWOS:000275419800013
Funding OrganizationBiomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union ; Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union ; Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union ; Biomedical Research Council of Singapore [R-08-1-21-19-585, R-154-000-371-305, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000285-112]; Lee Hiok Kwee donation fund [R-154-000-153-720]; Deutsche Forschungsgemeinschaft ; Commission of the European Union
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13643
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore
Recommended Citation
GB/T 7714
Hong, Ni,Li, Mingyou,Zeng, Zhiqiang,et al. Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2010,67(7):1189-1202.
APA Hong, Ni.,Li, Mingyou.,Zeng, Zhiqiang.,Yi, Meisheng.,Deng, Jiaorong.,...&Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore.(2010).Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos.CELLULAR AND MOLECULAR LIFE SCIENCES,67(7),1189-1202.
MLA Hong, Ni,et al."Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos".CELLULAR AND MOLECULAR LIFE SCIENCES 67.7(2010):1189-1202.
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