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Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization
Li, N.; Zhang, J.; Zhang, L. Q.; Nie, P.; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2010
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Volume33Issue:5Pages:403-412
AbstractFlavobacterium columnare is the causative agent of columnaris disease. Different genetic groups of F. columnare show to some extent different degrees of virulence. To identify genetic differences between the high virulence strain G(4) and the low virulence strain G(18) of F. columnare, suppression subtractive hybridization was used. A total of 46 genes were identified from the virulent strain G(4), 35 of which showed some degree of homology with known proteins and can be classified into 11 categories: DNA replication or recombination proteins, inorganic ion transport proteins, outer membrane proteins, enterotoxin, binding proteins, YD repeat proteins, transposase, chaperon, signal transduction-related proteins, regulatory proteins, metabolism-related proteins. Several putative virulence factors identified in other bacteria could also be identified in the virulent strain G(4), such as ferrous iron transport protein, TonB-dependent receptor, transposases, as well as ABC transporter permease protein. The flanking region of a putative transposase ISFclI was analysed, and a putative Rhs element was located at the downstream of the putative transposase. The analysis of isfclI gene in 24 strains of F. columnare isolated in China revealed that 11 strains have isfclI, and all the strains from Zhaoqing, Anhui and Qingjiang have isfclI.
KeywordFlavobacterium Columnare Pathogen Suppression Subtractive Hybridization Ssh Virulence
Department[Li, N.; Zhang, J.; Zhang, L. Q.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Li, N.; Zhang, J.; Zhang, L. Q.; Nie, P.] Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Li, N.; Zhang, J.; Zhang, L. Q.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Basic Research Programme of China [2009CB118703] ; National Basic Research Programme of China [2009CB118703] ; National Basic Research Programme of China [2009CB118703] ; National Basic Research Programme of China [2009CB118703]
Indexed BySCI
Language英语
WOS IDWOS:000276465300003
Funding OrganizationNational Basic Research Programme of China [2009CB118703] ; National Basic Research Programme of China [2009CB118703] ; National Basic Research Programme of China [2009CB118703] ; National Basic Research Programme of China [2009CB118703]
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13637
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Li, N.,Zhang, J.,Zhang, L. Q.,et al. Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization[J]. JOURNAL OF FISH DISEASES,2010,33(5):403-412.
APA Li, N.,Zhang, J.,Zhang, L. Q.,Nie, P.,&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.(2010).Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization.JOURNAL OF FISH DISEASES,33(5),403-412.
MLA Li, N.,et al."Difference in genes between a high virulence strain G(4) and a low virulence strain G(18) of Flavobacterium columnare by using suppression subtractive hybridization".JOURNAL OF FISH DISEASES 33.5(2010):403-412.
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