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学科主题: Immunology; Veterinary Sciences
题名: The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella
作者: Xu, Qiao Q.1, 2, 3; Chang, Ming X.1; Xiao, Fan S.1; Huang, Bei1; Nie, Pin1
通讯作者: Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
关键词: IRF-5 ; Genome ; Expression ; GCHV ; Grass carp ; Ctenopharyngodon idella
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2010-04-15
DOI: 10.1016/j.vetimm.2009.10.020
卷: 134, 期:3-4, 页:269-278
收录类别: SCI
文章类型: Article
部门归属: [Xu, Qiao Q.; Chang, Ming X.; Xiao, Fan S.; Huang, Bei; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Xu, Qiao Q.] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China; [Xu, Qiao Q.] Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Veterinary Sciences
研究领域[WOS]: Immunology ; Veterinary Sciences
摘要: The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune response and in the regulation of DNA damage-induced apoptosis. In the present study, the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenophonyngodon idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and 95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075 bp consisting of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined. The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV) induced the expression of gcIRF-5 in spleen and head kidney. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要: The interferon regulatory factor 5 (IRF-5) is known to be involved in the innate immune response and in the regulation of DNA damage-induced apoptosis. In the present study, the cDNA and genomic sequences of IRF-5 were identified in grass carp (Ctenophonyngodon idella). The cDNA of grass carp IRF-5 (gcIRF-5) contains an open reading frame (ORF) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to IRF-5 homologues from mammals, amphibian, avian and fish, and 96.2% and 95.0% identity to zebrafish IRF-5 in the DNA-binding domain (DBD) and IRF association domain (IAD), respectively. The genomic DNA sequence of gcIRF-5 contains 6075 bp consisting of 9 exons and 8 introns. The expression of gcIRF-5 was observed in all organs examined. The analysis of real-time quantitative RT-PCR revealed that grass carp reovirus (GCRV) induced the expression of gcIRF-5 in spleen and head kidney. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: INTERFERON REGULATORY FACTOR ; FISH SINIPERCA-CHUATSI ; MOLECULAR-CLONING ; RAINBOW-TROUT ; TRANSCRIPTION ; ACTIVATION ; ZEBRAFISH ; INDUCTION ; SEQUENCE ; DNA
语种: 英语
WOS记录号: WOS:000276518700015
ISSN号: 0165-2427
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13635
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
3.Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China

Recommended Citation:
Xu, Qiao Q.; Chang, Ming X.; Xiao, Fan S.; Huang, Bei; Nie, Pin.The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2010,134(3-4):269-278
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