IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella
Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Zou, J.; Secombes, C. J.; Peng, K. M.; Nie, P.; Peng, KM, Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China
2010
Source PublicationVETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
ISSN0165-2427
Volume134Issue:40241Pages:199-207
AbstractInterferon gamma (IFN-gamma), the only member of the type 11 class of interferons, has been identified in teleost fish. In addition to the IFN-gamma gene, fish possess an IFN-gamma related gene (IFN-gamma rel) neighbouring the authentic IFN-gamma gene in the genome. In the present study, the cDNA sequence encoding 167 amino acids of IFN-gamma rel and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted protein sequence of grass carp IFN-gamma rel (gcIFN-gamma rel) showed 63% and 50% identities to zebrafish and common carp IFN-gamma rel (previously termed as IFN-gamma 1), respectively. The IFN-gamma rel gene consists of 4 exons, with 3 intervening introns, spanning approximately 2 kb of genomic sequence. The gcIFN-gamma rel gene did not contain any polymorphic DNA repeats in the introns. Realtime PCR analysis showed that grass carp reovirus induced a high and long lasting (from days 1 to 7) expression of gcIFN-gamma rel in spleen. The expression of gcIFN-gamma rel in blood, head kidney, trunk kidney and spleen was also increased by bacterial peptidoglycan (PGN), lipopolysaccharide (LPS) and the interferon inducer polyI:C. The highest induction of gcIFN-gamma rel expression by PGN was observed in spleen, then in blood and head kidney. Further analysis of the expression patterns of gcIFN-gamma rel and PGN receptors, nucleotide oligomerization domains (NOD) 1 and 2, may suggest that IFN-gamma rel was possibly activated in a NOD2-dependent mechanism. (C) 2009 Elsevier B.V. All rights reserved.
KeywordGrass Carp Ifn-gamma Related Gene Reovirus Peptidoglycan Nucleotide Oligomerization Domain
Department[Chen, W. Q.; Peng, K. M.] Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China; [Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China; [Zou, J.; Secombes, C. J.] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
Subject AreaImmunology ; Veterinary Sciences
Funding OrganizationNational Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh
Indexed BySCI
Language英语
WOS IDWOS:000276518700007
Funding OrganizationNational Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh ; National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13633
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorPeng, KM, Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Chen, W. Q.,Xu, Q. Q.,Chang, M. X.,et al. Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2010,134(40241):199-207.
APA Chen, W. Q..,Xu, Q. Q..,Chang, M. X..,Zou, J..,Secombes, C. J..,...&Peng, KM, Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China.(2010).Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,134(40241),199-207.
MLA Chen, W. Q.,et al."Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 134.40241(2010):199-207.
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