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学科主题: Immunology; Veterinary Sciences
题名: Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella
作者: Chen, W. Q.1, 2, 3; Xu, Q. Q.2, 3; Chang, M. X.2, 3; Zou, J.4; Secombes, C. J.4; Peng, K. M.1; Nie, P.2, 3
通讯作者: Peng, KM, Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China
关键词: Grass carp ; IFN-gamma related gene ; Reovirus ; Peptidoglycan ; Nucleotide oligomerization domain
刊名: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
发表日期: 2010-04-15
DOI: 10.1016/j.vetimm.2009.09.007
卷: 134, 期:3-4, 页:199-207
收录类别: SCI
文章类型: Article
部门归属: [Chen, W. Q.; Peng, K. M.] Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China; [Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China; [Zou, J.; Secombes, C. J.] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China (NSFC) [U0631010, 30830083, 30711130225]; Royal Society of Edinburgh
类目[WOS]: Immunology ; Veterinary Sciences
研究领域[WOS]: Immunology ; Veterinary Sciences
摘要: Interferon gamma (IFN-gamma), the only member of the type 11 class of interferons, has been identified in teleost fish. In addition to the IFN-gamma gene, fish possess an IFN-gamma related gene (IFN-gamma rel) neighbouring the authentic IFN-gamma gene in the genome. In the present study, the cDNA sequence encoding 167 amino acids of IFN-gamma rel and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted protein sequence of grass carp IFN-gamma rel (gcIFN-gamma rel) showed 63% and 50% identities to zebrafish and common carp IFN-gamma rel (previously termed as IFN-gamma 1), respectively. The IFN-gamma rel gene consists of 4 exons, with 3 intervening introns, spanning approximately 2 kb of genomic sequence. The gcIFN-gamma rel gene did not contain any polymorphic DNA repeats in the introns. Realtime PCR analysis showed that grass carp reovirus induced a high and long lasting (from days 1 to 7) expression of gcIFN-gamma rel in spleen. The expression of gcIFN-gamma rel in blood, head kidney, trunk kidney and spleen was also increased by bacterial peptidoglycan (PGN), lipopolysaccharide (LPS) and the interferon inducer polyI:C. The highest induction of gcIFN-gamma rel expression by PGN was observed in spleen, then in blood and head kidney. Further analysis of the expression patterns of gcIFN-gamma rel and PGN receptors, nucleotide oligomerization domains (NOD) 1 and 2, may suggest that IFN-gamma rel was possibly activated in a NOD2-dependent mechanism. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要: Interferon gamma (IFN-gamma), the only member of the type 11 class of interferons, has been identified in teleost fish. In addition to the IFN-gamma gene, fish possess an IFN-gamma related gene (IFN-gamma rel) neighbouring the authentic IFN-gamma gene in the genome. In the present study, the cDNA sequence encoding 167 amino acids of IFN-gamma rel and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted protein sequence of grass carp IFN-gamma rel (gcIFN-gamma rel) showed 63% and 50% identities to zebrafish and common carp IFN-gamma rel (previously termed as IFN-gamma 1), respectively. The IFN-gamma rel gene consists of 4 exons, with 3 intervening introns, spanning approximately 2 kb of genomic sequence. The gcIFN-gamma rel gene did not contain any polymorphic DNA repeats in the introns. Realtime PCR analysis showed that grass carp reovirus induced a high and long lasting (from days 1 to 7) expression of gcIFN-gamma rel in spleen. The expression of gcIFN-gamma rel in blood, head kidney, trunk kidney and spleen was also increased by bacterial peptidoglycan (PGN), lipopolysaccharide (LPS) and the interferon inducer polyI:C. The highest induction of gcIFN-gamma rel expression by PGN was observed in spleen, then in blood and head kidney. Further analysis of the expression patterns of gcIFN-gamma rel and PGN receptors, nucleotide oligomerization domains (NOD) 1 and 2, may suggest that IFN-gamma rel was possibly activated in a NOD2-dependent mechanism. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: TROUT ONCORHYNCHUS-MYKISS ; TOLL-LIKE RECEPTOR ; INTERFERON-GAMMA ; ATLANTIC SALMON ; CHANNEL CATFISH ; DANIO-RERIO ; IDENTIFICATION ; RESISTANCE ; FISH ; ZEBRAFISH
语种: 英语
WOS记录号: WOS:000276518700007
ISSN号: 0165-2427
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13633
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Anim Sci & Vet Med, Wuhan 430070, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China
4.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland

Recommended Citation:
Chen, W. Q.; Xu, Q. Q.; Chang, M. X.; Zou, J.; Secombes, C. J.; Peng, K. M.; Nie, P..Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gamma rel) in grass carp Ctenopharyngodon idella,VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2010,134(3-4):199-207
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