中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
学科主题: Immunology
题名: Intron-Containing Type I and Type III IFN Coexist in Amphibians: Refuting the Concept That a Retroposition Event Gave Rise to Type I IFNs
作者: Qi, Zhitao1, 2; Nie, Pin1; Secombes, Chris J.3; Zou, Jun3
通讯作者: Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
关键词: INTERFERON GENES ; RECEPTOR ; IDENTIFICATION ; ZEBRAFISH ; VERTEBRATES ; FAMILY ; FISH ; EXPRESSION ; CYTOKINES ; SYSTEM
刊名: JOURNAL OF IMMUNOLOGY
发表日期: 2010-05-01
DOI: 10.4049/jimmunol.0903374
卷: 184, 期:9, 页:5038-5046
收录类别: SCI
文章类型: Article
部门归属: [Qi, Zhitao; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Qi, Zhitao] Yancheng Inst Technol, Chem & Biol Engn Coll, Jiangsu, Peoples R China; [Secombes, Chris J.; Zou, Jun] Univ Aberdeen, Scottish Fish Immunol Res Ctr, Inst Biol & Environm Sci, Aberdeen, Scotland
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China ; Government of Guangdong Province [U0631010]; National Basic Research Program of China (973 Program) [2009CB118703]; Royal Society of Edinburgh
类目[WOS]: Immunology
研究领域[WOS]: Immunology
摘要: Type I and III IFNs are structurally related cytokines with similar antiviral functions. They have different genomic organizations and bind to distinct receptor complexes. It has been vigorously debated whether the recently identified intron containing IFN genes in fish and amphibians belong to the type I or HI IFN family or diverged from a common ancestral gene, that subsequently gave rise to both types. In this report, we have identified intron containing type III IFN genes that are tandemly linked in the Xenopus tropicalis genome and hence demonstrate for the first time that intron containing type I and III genes diverged relatively early in vertebrate evolution, and at least by the appearance of early tetrapods, a transition period when vertebrates migrated from an aquatic environment to land. Our data also suggest that the intronless type I IFN genes seen in reptiles, birds, and mammals have originated from a type I IFN transcript via a retroposition event that led to the disappearance of intron-containing type I IFN genes in modern vertebrates. In vivo and in vitro studies in this paper show that the Xenopus type III IFNs and their cognate receptor are ubiquitously expressed in tissues and primary splenocytes and can be upregulated by stimulation with synthetic double-stranded RNA, suggesting they are involved in antiviral defense in amphibians. The Journal of Immunology, 2010, 184: 5038-5046.
英文摘要: Type I and III IFNs are structurally related cytokines with similar antiviral functions. They have different genomic organizations and bind to distinct receptor complexes. It has been vigorously debated whether the recently identified intron containing IFN genes in fish and amphibians belong to the type I or HI IFN family or diverged from a common ancestral gene, that subsequently gave rise to both types. In this report, we have identified intron containing type III IFN genes that are tandemly linked in the Xenopus tropicalis genome and hence demonstrate for the first time that intron containing type I and III genes diverged relatively early in vertebrate evolution, and at least by the appearance of early tetrapods, a transition period when vertebrates migrated from an aquatic environment to land. Our data also suggest that the intronless type I IFN genes seen in reptiles, birds, and mammals have originated from a type I IFN transcript via a retroposition event that led to the disappearance of intron-containing type I IFN genes in modern vertebrates. In vivo and in vitro studies in this paper show that the Xenopus type III IFNs and their cognate receptor are ubiquitously expressed in tissues and primary splenocytes and can be upregulated by stimulation with synthetic double-stranded RNA, suggesting they are involved in antiviral defense in amphibians. The Journal of Immunology, 2010, 184: 5038-5046.
关键词[WOS]: INTERFERON GENES ; RECEPTOR ; IDENTIFICATION ; ZEBRAFISH ; VERTEBRATES ; FAMILY ; FISH ; EXPRESSION ; CYTOKINES ; SYSTEM
语种: 英语
WOS记录号: WOS:000277093000056
ISSN号: 0022-1767
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13629
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
J Immunol-2010-Qi-5038-46.pdf(1171KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Yancheng Inst Technol, Chem & Biol Engn Coll, Jiangsu, Peoples R China
3.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Inst Biol & Environm Sci, Aberdeen, Scotland

Recommended Citation:
Qi, Zhitao; Nie, Pin; Secombes, Chris J.; Zou, Jun.Intron-Containing Type I and Type III IFN Coexist in Amphibians: Refuting the Concept That a Retroposition Event Gave Rise to Type I IFNs,JOURNAL OF IMMUNOLOGY,2010,184(9):5038-5046
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qi, Zhitao]'s Articles
[Nie, Pin]'s Articles
[Secombes, Chris J.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qi, Zhitao]‘s Articles
[Nie, Pin]‘s Articles
[Secombes, Chris J.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: J Immunol-2010-Qi-5038-46.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace