| Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp | |
| Jang, Songhun; Liu, Hang; Su, Jianguo; Dong, Feng; Xiong, Feng; Liao, Lanjie; Wang, Yaping; Zhu, Zuoyan; Wang, YP, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China | |
| 2010 | |
| Source Publication | MARINE BIOTECHNOLOGY
 |
| ISSN | 1436-2228 |
| Volume | 12Issue:3Pages:261-266 |
| Abstract | Bacterial artificial chromosome (BAC) library is an important tool in genomic research. We constructed two libraries from the genomic DNA of grass carp (Ctenopharyngodon idellus) as a crucial part of the grass carp genome project. The libraries were constructed in the EcoRI and HindIII sites of the vector CopyControl pCC1BAC. The EcoRI library comprised 53,000 positive clones, and approximately 99.94% of the clones contained grass carp nuclear DNA inserts (average size, 139.7 kb) covering 7.4x haploid genome equivalents and 2% empty clones. Similarly, the HindIII library comprised 52,216 clones with approximately 99.82% probability of finding any genomic fragments containing single-copy genes; the average insert size was 121.5 kb with 2.8% insert-empty clones, thus providing genome coverage of 6.3x haploid genome equivalents of grass carp. We selected gene-specific probes for screening the target gene clones in the HindIII library. In all, we obtained 31 positive clones, which were identified for every gene, with an average of 6.2 BAC clones per gene probe. Thus, we succeeded in constructing the desired BAC libraries, which should provide an important foundation for future physical mapping and whole-genome sequencing in grass carp. |
| Keyword | Bacterial Artificial Chromosome Grass Carp Gene-specific Hybridization |
| Department | [Jang, Songhun; Liu, Hang; Su, Jianguo; Dong, Feng; Xiong, Feng; Liao, Lanjie; Wang, Yaping; Zhu, Zuoyan] Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Jang, Songhun] Kim IL Sung Univ, Coll Life Sci, Pyongyang, North Korea; [Liu, Hang; Dong, Feng; Xiong, Feng] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| Subject Area | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| Funding Organization | Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000278095400003 |
| Funding Organization | Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/13611 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Wang, YP, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| Recommended Citation GB/T 7714 | Jang, Songhun,Liu, Hang,Su, Jianguo,et al. Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp[J]. MARINE BIOTECHNOLOGY,2010,12(3):261-266. |
| APA | Jang, Songhun.,Liu, Hang.,Su, Jianguo.,Dong, Feng.,Xiong, Feng.,...&Wang, YP, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.(2010).Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp.MARINE BIOTECHNOLOGY,12(3),261-266. |
| MLA | Jang, Songhun,et al."Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp".MARINE BIOTECHNOLOGY 12.3(2010):261-266. |
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