IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp
Jang, Songhun; Liu, Hang; Su, Jianguo; Dong, Feng; Xiong, Feng; Liao, Lanjie; Wang, Yaping; Zhu, Zuoyan; Wang, YP, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2010
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Volume12Issue:3Pages:261-266
AbstractBacterial artificial chromosome (BAC) library is an important tool in genomic research. We constructed two libraries from the genomic DNA of grass carp (Ctenopharyngodon idellus) as a crucial part of the grass carp genome project. The libraries were constructed in the EcoRI and HindIII sites of the vector CopyControl pCC1BAC. The EcoRI library comprised 53,000 positive clones, and approximately 99.94% of the clones contained grass carp nuclear DNA inserts (average size, 139.7 kb) covering 7.4x haploid genome equivalents and 2% empty clones. Similarly, the HindIII library comprised 52,216 clones with approximately 99.82% probability of finding any genomic fragments containing single-copy genes; the average insert size was 121.5 kb with 2.8% insert-empty clones, thus providing genome coverage of 6.3x haploid genome equivalents of grass carp. We selected gene-specific probes for screening the target gene clones in the HindIII library. In all, we obtained 31 positive clones, which were identified for every gene, with an average of 6.2 BAC clones per gene probe. Thus, we succeeded in constructing the desired BAC libraries, which should provide an important foundation for future physical mapping and whole-genome sequencing in grass carp.
KeywordBacterial Artificial Chromosome Grass Carp Gene-specific Hybridization
Department[Jang, Songhun; Liu, Hang; Su, Jianguo; Dong, Feng; Xiong, Feng; Liao, Lanjie; Wang, Yaping; Zhu, Zuoyan] Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; [Jang, Songhun] Kim IL Sung Univ, Coll Life Sci, Pyongyang, North Korea; [Liu, Hang; Dong, Feng; Xiong, Feng] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
Funding OrganizationDevelopment Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084]
Indexed BySCI
Language英语
WOS IDWOS:000278095400003
Funding OrganizationDevelopment Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084] ; Development Plan of the State Key Fundamental Research of China [2009CB118701]; National Natural Science Foundation of China [30540084]
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13611
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, YP, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Jang, Songhun,Liu, Hang,Su, Jianguo,et al. Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp[J]. MARINE BIOTECHNOLOGY,2010,12(3):261-266.
APA Jang, Songhun.,Liu, Hang.,Su, Jianguo.,Dong, Feng.,Xiong, Feng.,...&Wang, YP, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.(2010).Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp.MARINE BIOTECHNOLOGY,12(3),261-266.
MLA Jang, Songhun,et al."Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp".MARINE BIOTECHNOLOGY 12.3(2010):261-266.
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