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The first non-mammalian CXCR5 in a teleost fish: molecular cloning and expression analysis in grass carp (Ctenopharyngodon idella)
Xu, Qiao Q.; Chang, Ming X.; Sun, Rong H.; Xiao, Fan S.; Nie, P.; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2010
Source PublicationBMC IMMUNOLOGY
ISSN1471-2172
Volume11Pages:-
AbstractBackground: Chemokines, a group of small and structurally related proteins, mediate chemotaxis of various cell types via chemokine receptors. In mammals, seven different CXC chemokine receptors denoted as CXCR1 to CXCR7 have been reported. However, the chemokine receptor CXCR5 has not been reported in other vertebrates. Results: In the present study, the genomic sequence of CXCR5 was isolated from the grass carp Ctenopharyngodon idella. The cDNA sequence of grass carp CXCR5 (gcCXCR5) consists of 1518 bp with a 43 bp 5' untranslated region (UTR) and a 332 bp 3' UTR, with an open reading frame of 1143 bp encoding 381 amino acids which are predicted to have seven transmembrane helices. The characteristic residues (DRYLAIVHA) and conserved cysteine residues are located in the extracellular regions and in the third to seventh transmembrane domains. The deduced amino acid sequence shows 37.6-66.6% identities with CXCR5 of mammals, avian and other fish species. The grass carp gene consists of two exons, with one intervening intron, spaced over 2081 bp of genomic sequence. Phylogenetic analysis clearly demonstrated that the gcCXCR5 is clustered with those in other teleost fish and then in chicken and mammals. Real-time PCR analysis showed that gcCXCR5 was expressed in all tested organs/tissues and its expression level was the highest in trunk kidney, followed by in the spleen. The expression of gcCXCR5 was significantly modulated by immunostimulants such as peptidoglycan (PGN), lipopolysaccharide (LPS), polyinosinic-polycytidylic acid sodium salt (Poly I: C) and phytohaemagglutinin (PHA). Conclusion: The cDNA and genomic sequences of CXCR5 have been successfully characterized in a teleost fish, the grass carp. The CXCR5 has in general a constitutive expression in organs/tissues examined, whereas its expression was significantly up-regulated in immune organs and down-regulated in brain, indicating its potential role in immune response and central nervous system.
KeywordProtein-coupled Receptor Central-nervous-system B-cell Differentiation Chemokine Receptors Migration Neurons Cxcr7/rdc1 Coreceptor Activation Homolog
Department[Xu, Qiao Q.; Chang, Ming X.; Sun, Rong H.; Xiao, Fan S.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Xu, Qiao Q.] Yangtze Univ, Sch Anim Sci, Jinzhou 434025, Hubei Province, Peoples R China
Subject AreaImmunology
Funding OrganizationNational Basic Research Program of China (973 Program) [2009CB118703] ; National Basic Research Program of China (973 Program) [2009CB118703] ; National Basic Research Program of China (973 Program) [2009CB118703] ; National Basic Research Program of China (973 Program) [2009CB118703]
Indexed BySCI
Language英语
WOS IDWOS:000279893400001
Funding OrganizationNational Basic Research Program of China (973 Program) [2009CB118703] ; National Basic Research Program of China (973 Program) [2009CB118703] ; National Basic Research Program of China (973 Program) [2009CB118703] ; National Basic Research Program of China (973 Program) [2009CB118703]
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13603
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Xu, Qiao Q.,Chang, Ming X.,Sun, Rong H.,et al. The first non-mammalian CXCR5 in a teleost fish: molecular cloning and expression analysis in grass carp (Ctenopharyngodon idella)[J]. BMC IMMUNOLOGY,2010,11:-.
APA Xu, Qiao Q.,Chang, Ming X.,Sun, Rong H.,Xiao, Fan S.,Nie, P.,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.(2010).The first non-mammalian CXCR5 in a teleost fish: molecular cloning and expression analysis in grass carp (Ctenopharyngodon idella).BMC IMMUNOLOGY,11,-.
MLA Xu, Qiao Q.,et al."The first non-mammalian CXCR5 in a teleost fish: molecular cloning and expression analysis in grass carp (Ctenopharyngodon idella)".BMC IMMUNOLOGY 11(2010):-.
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