中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
学科主题: Fisheries; Marine & Freshwater Biology
题名: Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides)
作者: Luo, Yu-Shan1, 2; Hu, Wei1; Liu, Xiao-Chun3; Lin, Hao-Ran3; Zhu, Zuo-Yan1
通讯作者: Hu, W, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Orange-spotted grouper ; Sox9 ; Sex reversal ; Expression pattern
刊名: AQUACULTURE
发表日期: 2010-08-15
DOI: 10.1016/j.aquaculture.2010.06.019
卷: 306, 期:1-4, 页:322-328
收录类别: SCI
文章类型: Article
部门归属: [Luo, Yu-Shan; Hu, Wei; Zhu, Zuo-Yan] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Luo, Yu-Shan] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Liu, Xiao-Chun; Lin, Hao-Ran] Zhong Shan Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [U0631007]; '863' High Technology Project [2006AA10Z141]
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
摘要: Sox9 is a key gene in male sex determination and gonad development. To study its potential function in the female-to-male sex reversal in orange-spotted grouper, we conducted the following studies. The Sox9 gene was cloned and full-length sequence of Sox9 mRNA was determined using the rapid amplification of cDNA ends method. The Sox9 mRNA consists of 3277 bp in size with a 328 bp 5' untranslated region and a 1511 bp 3' untranslated region. The 1437 bp opening reading frame encodes a 479 amino acid protein. The Sox9 gene contains 3 exons and 2 introns; the beginning and end of both introns conform to the "GT-AG" rule. RT-PCR analysis indicated that Sox9 mRNA was expressed in brain, kidney, heart, liver, muscle, stomach, intestine, spleen, testis and ovary in adult orange-spotted grouper. Artificial sex reversal was successfully performed by implanting a medicinal strip containing 17 alpha-methyltestosterone into the groupers. By real-time PCR, we found that Sox9 was weakly expressed in the ovary-stage gonads (before treatment). Once the sex reversal began (1 week after treatment), Sox9 mRNA expression level significantly increased. However, at 2 weeks after treatment, Sox9 mRNA expression level significantly decreased to a level that was only slightly higher than that before treatment Sox9 mRNA expression increased again at 4 weeks after treatment, and at 6 and 8 weeks, it was maintained at a high level close to that at 1 week after treatment. The results suggest that Sox9 may be one of the important factors initiating and maintaining the masculinization of orange-spotted grouper during sex reversal. (C) 2010 Elsevier B.V. All rights reserved.
英文摘要: Sox9 is a key gene in male sex determination and gonad development. To study its potential function in the female-to-male sex reversal in orange-spotted grouper, we conducted the following studies. The Sox9 gene was cloned and full-length sequence of Sox9 mRNA was determined using the rapid amplification of cDNA ends method. The Sox9 mRNA consists of 3277 bp in size with a 328 bp 5' untranslated region and a 1511 bp 3' untranslated region. The 1437 bp opening reading frame encodes a 479 amino acid protein. The Sox9 gene contains 3 exons and 2 introns; the beginning and end of both introns conform to the "GT-AG" rule. RT-PCR analysis indicated that Sox9 mRNA was expressed in brain, kidney, heart, liver, muscle, stomach, intestine, spleen, testis and ovary in adult orange-spotted grouper. Artificial sex reversal was successfully performed by implanting a medicinal strip containing 17 alpha-methyltestosterone into the groupers. By real-time PCR, we found that Sox9 was weakly expressed in the ovary-stage gonads (before treatment). Once the sex reversal began (1 week after treatment), Sox9 mRNA expression level significantly increased. However, at 2 weeks after treatment, Sox9 mRNA expression level significantly decreased to a level that was only slightly higher than that before treatment Sox9 mRNA expression increased again at 4 weeks after treatment, and at 6 and 8 weeks, it was maintained at a high level close to that at 1 week after treatment. The results suggest that Sox9 may be one of the important factors initiating and maintaining the masculinization of orange-spotted grouper during sex reversal. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: TELEOST FISH ; GONADAL DIFFERENTIATION ; CAMPOMELIC DYSPLASIA ; TESTIS DEVELOPMENT ; GENE STRUCTURE ; SRY ; LATIPES ; MEDAKA ; MICE
语种: 英语
WOS记录号: WOS:000280735200042
ISSN号: 0044-8486
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13597
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides).pdf(1854KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Zhong Shan Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Luo, Yu-Shan; Hu, Wei; Liu, Xiao-Chun; Lin, Hao-Ran; Zhu, Zuo-Yan.Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides),AQUACULTURE,2010,306(1-4):322-328
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Luo, Yu-Shan]'s Articles
[Hu, Wei]'s Articles
[Liu, Xiao-Chun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Luo, Yu-Shan]‘s Articles
[Hu, Wei]‘s Articles
[Liu, Xiao-Chun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Molecular cloning and mRNA expression pattern of Sox9 during sex reversal in orange-spotted grouper (Epinephelus coioides).pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace