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Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus
Jia, W. Z.; Shang, N.; Guo, Q. L.; Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
Volume36Issue:3Pages:451-459
AbstractLectins are sugar-binding proteins that mediate pathogen recognition and cell-cell interactions. A rhamnose-binding lectin (RBL) gene and its promoter region have been cloned and characterized from snakehead Channa argus. From the transcription initiation site, snakehead rhamnose-binding lectin (SHL) gene extends 2,382 bp to the end of the 3' untranslated region (UTR), and contains nine exons and eight introns. The open reading frame (ORF) of the SHL transcript has 675 bp which encodes 224 amino acids. The molecular structure of SHL is composed of two tandem repeat carbohydrate recognition domains (CRD) with 35% internal identity. Analysis of the gene organization of SHL indicates that the ancestral gene of RBL may diverge and evolve by exon shuffling and gene duplication, producing new forms to play their own roles in various organisms. The characteristics of SHL gene 5' flanking region are the presence of consensus nuclear factor of interleukin 6 (NF-IL6) and IFN-gamma activation (GAS) sites. The results provide indirect evidence that up-regulation of SHL expression may be induced in response to inflammatory stimuli, such as lipopolysaccharide (LPS), interleukin 6 (IL-6), and interferon gamma (IFN-gamma). The transcript of SHL mRNA was expressed in the head kidney, posterior kidney, spleen, liver, intestine, heart, muscle, and ovary. No tissue-specific expressive pattern is different from reported STLs, WCLs, and PFLs, suggesting that different types of RBLs exist in species-specific fish that have evolved and adapted to their surroundings.
KeywordRhamnose-binding Lectin Carbohydrate Recognition Domain Gene Organization Promoter Snakehead Channa Argus
Department[Jia, W. Z.; Shang, N.; Guo, Q. L.] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Jia, W. Z.] Guangdong Pharmaceut Univ, Sch Life Sci, Guangzhou 510006, Guangdong, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
Funding OrganizationNational Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022]
Indexed BySCI
Language英语
WOS IDWOS:000281087300016
Funding OrganizationNational Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022] ; National Natural Science Foundation of China [30371091, 30871912]; National Basic Research Program of China [2009CB118704]; Doctoral Scientific Research Startup of Guangdong Pharmaceutical University [43555022]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13595
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jia, W. Z.,Shang, N.,Guo, Q. L.,et al. Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2010,36(3):451-459.
APA Jia, W. Z.,Shang, N.,Guo, Q. L.,&Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2010).Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus.FISH PHYSIOLOGY AND BIOCHEMISTRY,36(3),451-459.
MLA Jia, W. Z.,et al."Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus".FISH PHYSIOLOGY AND BIOCHEMISTRY 36.3(2010):451-459.
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