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学科主题: Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
题名: Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella
作者: Xiao, F. S.1, 2; Wang, Y. P.1; Yan, W.1; Chang, M. X.1; Yao, W. J.1; Xu, Q. Q.1; Wang, X. X.1; Gao, Q.1; Nie, P.1
通讯作者: Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
关键词: Immunoglobulin (Ig) ; IgZ ; IgM ; IgD ; IgH locus ; Grass carp, Ctenopharyngodon idella
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2010-10-01
DOI: 10.1016/j.fsi.2010.06.004
卷: 29, 期:4, 页:594-599
收录类别: SCI
文章类型: Article
部门归属: [Xiao, F. S.; Wang, Y. P.; Yan, W.; Chang, M. X.; Yao, W. J.; Xu, Q. Q.; Wang, X. X.; Gao, Q.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Xiao, F. S.] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Programme (973 Programme) of China [2009CB118703]
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: The cDNA and genomic sequences of IgD and IgZ were characterized in grass carp Ctenopharyngodon idella in the present study, and with the identification of a BAC clone covering zeta, mu, and delta genes, the IgH locus containing these Ig genes and other V, D, J genes was also illustrated in this fish. Secretory and membrane-bound IgZ were identified, with two transmembrane exons spliced within the CH4 exon, as reported in IgM of mammals and IgZ in other teleost fish. The first and second constant domains of IgZ shows more than 90% nucleotide identity with respective domains of grass carp IgM. The IgD has a structure of delta 1-(delta 2-delta 3-delta 4)(2)-delta 5-delta 6-delta 7-TM-UTR, with the repeat of delta 2-delta 3-delta 4; but intron was not found between the two repeat, i.e. between the first delta 2-delta 3-delta 4 (delta 2.1-delta 3.1-delta 4.1) and the second delta 2-delta 3-delta 4 (delta 2.2-delta 3.2-delta 4.2), and the intron between delta 3.1 and delta 4.1 was much shorter than the intron between delta 3.2 and delta 4.2. The genomic organization of the IgH locus has a pattern of Vn-Dn-Jn-C zeta-Dn-Jn-C mu-C delta, as reported in other teleost fish. Thirteen V-H, fourteen D, and twelve J(H) genes were observed in this locus, with the similarity of three D segments and four J(H) segments being the same in the upstream of C zeta, and C mu. The transcriptional enhancer located at the mu-delta intergenic region was also analyzed and it seems possible that this enhancer is functional as verified in zebrafish and channel catfish. (C) 2010 Elsevier Ltd. All rights reserved.
英文摘要: The cDNA and genomic sequences of IgD and IgZ were characterized in grass carp Ctenopharyngodon idella in the present study, and with the identification of a BAC clone covering zeta, mu, and delta genes, the IgH locus containing these Ig genes and other V, D, J genes was also illustrated in this fish. Secretory and membrane-bound IgZ were identified, with two transmembrane exons spliced within the CH4 exon, as reported in IgM of mammals and IgZ in other teleost fish. The first and second constant domains of IgZ shows more than 90% nucleotide identity with respective domains of grass carp IgM. The IgD has a structure of delta 1-(delta 2-delta 3-delta 4)(2)-delta 5-delta 6-delta 7-TM-UTR, with the repeat of delta 2-delta 3-delta 4; but intron was not found between the two repeat, i.e. between the first delta 2-delta 3-delta 4 (delta 2.1-delta 3.1-delta 4.1) and the second delta 2-delta 3-delta 4 (delta 2.2-delta 3.2-delta 4.2), and the intron between delta 3.1 and delta 4.1 was much shorter than the intron between delta 3.2 and delta 4.2. The genomic organization of the IgH locus has a pattern of Vn-Dn-Jn-C zeta-Dn-Jn-C mu-C delta, as reported in other teleost fish. Thirteen V-H, fourteen D, and twelve J(H) genes were observed in this locus, with the similarity of three D segments and four J(H) segments being the same in the upstream of C zeta, and C mu. The transcriptional enhancer located at the mu-delta intergenic region was also analyzed and it seems possible that this enhancer is functional as verified in zebrafish and channel catfish. (C) 2010 Elsevier Ltd. All rights reserved.
关键词[WOS]: IMMUNOGLOBULIN GENES ; E-MU-3' ENHANCER ; CHANNEL CATFISH ; MESSENGER-RNA ; REGION GENES ; TELEOST FISH ; EXPRESSION ; ISOTYPE ; EVOLUTION ; DOMAINS
语种: 英语
WOS记录号: WOS:000281500500007
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13591
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Xiao, F. S.; Wang, Y. P.; Yan, W.; Chang, M. X.; Yao, W. J.; Xu, Q. Q.; Wang, X. X.; Gao, Q.; Nie, P..Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella,FISH & SHELLFISH IMMUNOLOGY,2010,29(4):594-599
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