IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella
Xiao, F. S.; Wang, Y. P.; Yan, W.; Chang, M. X.; Yao, W. J.; Xu, Q. Q.; Wang, X. X.; Gao, Q.; Nie, P.; Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2010
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume29Issue:4Pages:594-599
AbstractThe cDNA and genomic sequences of IgD and IgZ were characterized in grass carp Ctenopharyngodon idella in the present study, and with the identification of a BAC clone covering zeta, mu, and delta genes, the IgH locus containing these Ig genes and other V, D, J genes was also illustrated in this fish. Secretory and membrane-bound IgZ were identified, with two transmembrane exons spliced within the CH4 exon, as reported in IgM of mammals and IgZ in other teleost fish. The first and second constant domains of IgZ shows more than 90% nucleotide identity with respective domains of grass carp IgM. The IgD has a structure of delta 1-(delta 2-delta 3-delta 4)(2)-delta 5-delta 6-delta 7-TM-UTR, with the repeat of delta 2-delta 3-delta 4; but intron was not found between the two repeat, i.e. between the first delta 2-delta 3-delta 4 (delta 2.1-delta 3.1-delta 4.1) and the second delta 2-delta 3-delta 4 (delta 2.2-delta 3.2-delta 4.2), and the intron between delta 3.1 and delta 4.1 was much shorter than the intron between delta 3.2 and delta 4.2. The genomic organization of the IgH locus has a pattern of Vn-Dn-Jn-C zeta-Dn-Jn-C mu-C delta, as reported in other teleost fish. Thirteen V-H, fourteen D, and twelve J(H) genes were observed in this locus, with the similarity of three D segments and four J(H) segments being the same in the upstream of C zeta, and C mu. The transcriptional enhancer located at the mu-delta intergenic region was also analyzed and it seems possible that this enhancer is functional as verified in zebrafish and channel catfish. (C) 2010 Elsevier Ltd. All rights reserved.
KeywordImmunoglobulin (Ig) Igz Igm Igd Igh Locus Grass Carp, Ctenopharyngodon Idella
Department[Xiao, F. S.; Wang, Y. P.; Yan, W.; Chang, M. X.; Yao, W. J.; Xu, Q. Q.; Wang, X. X.; Gao, Q.; Nie, P.] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Xiao, F. S.] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Basic Research Programme (973 Programme) of China [2009CB118703] ; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Basic Research Programme (973 Programme) of China [2009CB118703]
Indexed BySCI
Language英语
WOS IDWOS:000281500500007
Funding OrganizationNational Basic Research Programme (973 Programme) of China [2009CB118703] ; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Basic Research Programme (973 Programme) of China [2009CB118703]
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Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13591
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Xiao, F. S.,Wang, Y. P.,Yan, W.,et al. Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(4):594-599.
APA Xiao, F. S..,Wang, Y. P..,Yan, W..,Chang, M. X..,Yao, W. J..,...&Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.(2010).Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella.FISH & SHELLFISH IMMUNOLOGY,29(4),594-599.
MLA Xiao, F. S.,et al."Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella".FISH & SHELLFISH IMMUNOLOGY 29.4(2010):594-599.
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