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学科主题: Genetics & Heredity
题名: Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid
作者: Zhu, Hua Ping1; Lu, Mai Xin1; Gao, Feng Ying1; Huang, Zhang Han1; Yang, Li Ping1; Gui, Jian Fang2
通讯作者: Lu, MX, Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
关键词: karyotype ; 5S rDNA ; NORs ; fluorescent in situ hybridization (FISH) ; tilapia
刊名: JOURNAL OF GENETICS
发表日期: 2010-08-01
卷: 89, 期:2, 页:163-171
收录类别: SCI
文章类型: Article
部门归属: [Zhu, Hua Ping; Lu, Mai Xin; Gao, Feng Ying; Huang, Zhang Han; Yang, Li Ping] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Gui, Jian Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03]
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
摘要: In this study, classical and molecular cytogenetic analyses were performed in tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing O mossambicus female x O. u hornorum male. An identical karyotype ((2n = 44, NF (total number of chromosomal arms) = 50) was obtained from three examined tilapia samples. Genomic organization analysis of 5S rDNA revealed two different types of 5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals on six chromosomes of all the analysed metaphases from the three tilapia samples. Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence signals were observed on three chromosome pairs in all analysed metaphases of the three tilapia samples. The correlation between 45 rDNA localization and nucleolar organizer regions (NORs) was confirmed by silver nitrate staining in tilapia fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were verified by two different colour FISH probes. Briefly, the current data provide an insights for hybridization projects and breeding improvement of tilapias.
英文摘要: In this study, classical and molecular cytogenetic analyses were performed in tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing O mossambicus female x O. u hornorum male. An identical karyotype ((2n = 44, NF (total number of chromosomal arms) = 50) was obtained from three examined tilapia samples. Genomic organization analysis of 5S rDNA revealed two different types of 5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals on six chromosomes of all the analysed metaphases from the three tilapia samples. Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence signals were observed on three chromosome pairs in all analysed metaphases of the three tilapia samples. The correlation between 45 rDNA localization and nucleolar organizer regions (NORs) was confirmed by silver nitrate staining in tilapia fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were verified by two different colour FISH probes. Briefly, the current data provide an insights for hybridization projects and breeding improvement of tilapias.
关键词[WOS]: RIBOSOMAL-RNA GENES ; CARASSIUS-AURATUS-GIBELIO ; IN-SITU HYBRIDIZATION ; NILOTICUS GENOME ; PERCIFORMES ; CICHLIDAE ; TILAPIA ; FISHES ; DNA ; EVOLUTION
语种: 英语
WOS记录号: WOS:000281515700005
ISSN号: 0022-1333
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13589
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhu, Hua Ping; Lu, Mai Xin; Gao, Feng Ying; Huang, Zhang Han; Yang, Li Ping; Gui, Jian Fang.Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid,JOURNAL OF GENETICS,2010,89(2):163-171
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文件名: Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid.pdf
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