IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid
Zhu, Hua Ping; Lu, Mai Xin; Gao, Feng Ying; Huang, Zhang Han; Yang, Li Ping; Gui, Jian Fang; Lu, MX, Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
2010
Source PublicationJOURNAL OF GENETICS
ISSN0022-1333
Volume89Issue:2Pages:163-171
AbstractIn this study, classical and molecular cytogenetic analyses were performed in tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing O mossambicus female x O. u hornorum male. An identical karyotype ((2n = 44, NF (total number of chromosomal arms) = 50) was obtained from three examined tilapia samples. Genomic organization analysis of 5S rDNA revealed two different types of 5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals on six chromosomes of all the analysed metaphases from the three tilapia samples. Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence signals were observed on three chromosome pairs in all analysed metaphases of the three tilapia samples. The correlation between 45 rDNA localization and nucleolar organizer regions (NORs) was confirmed by silver nitrate staining in tilapia fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were verified by two different colour FISH probes. Briefly, the current data provide an insights for hybridization projects and breeding improvement of tilapias.
KeywordKaryotype 5s Rdna Nors Fluorescent In Situ Hybridization (Fish) Tilapia
Department[Zhu, Hua Ping; Lu, Mai Xin; Gao, Feng Ying; Huang, Zhang Han; Yang, Li Ping] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China; [Gui, Jian Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaGenetics & Heredity
Funding OrganizationNational Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03] ; National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03] ; National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03] ; National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03]
Indexed BySCI
Language英语
WOS IDWOS:000281515700005
Funding OrganizationNational Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03] ; National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03] ; National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03] ; National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13589
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLu, MX, Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Hua Ping,Lu, Mai Xin,Gao, Feng Ying,et al. Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid[J]. JOURNAL OF GENETICS,2010,89(2):163-171.
APA Zhu, Hua Ping.,Lu, Mai Xin.,Gao, Feng Ying.,Huang, Zhang Han.,Yang, Li Ping.,...&Lu, MX, Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China.(2010).Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid.JOURNAL OF GENETICS,89(2),163-171.
MLA Zhu, Hua Ping,et al."Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid".JOURNAL OF GENETICS 89.2(2010):163-171.
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