IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Dynamic Distribution of Spindlin in Nucleoli, Nucleoplasm and Spindle From Primary Oocytes to Mature Eggs and its Critical Function for Oocyte-to-Embryo Transition in Gibel Carp
Sun, Min; Li, Zhi; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China
2010
Source PublicationJOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY
ISSN1932-5223
Volume313AIssue:8Pages:461-473
AbstractSpindlin (Spin) was thought as a maternal-effect factor associated with meiotic spindle. Its role for the oocyte-to-embryo transition was suggested in mouse, but its direct evidence for the function had been not obtained in other vertebrates. In this study, we used the CagSpin-specific antibody to investigate CagSpin expression pattern and distribution during oogenesis of gibel carp (Carassius auratus gibelio). First, the oocyte-specific expression pattern and dynamic distribution was revealed in nucleoli, nucleoplasm, and spindle from primary oocytes to mature eggs by immunofluorescence localization. In primary oocytes and growth stage oocytes, CagSpin accumulates in nucleoli in increasing numbers along with the oocyte growth, and its disassembly occurs in vitellogenic oocytes, which implicates that CagSpin may be a major component of a large number of nucleoli in fish growth oocytes. Then, co-localization of CagSpin and beta-tubulin was revealed in meiotic spindle of mature egg, indicating that CagSpin is one spindle-associated factor. Moreover, microinjection of CagSpin-specific antibody into the fertilized eggs blocked the first cleavage, and found that the CagSpin depletion resulted in spindle assembly disturbance. Thereby, our study provided the first direct evidence for the critical oocyte-to-embryo transition function of Spin in vertebrates, and confirmed that Spin is one important maternal-effect factor that participates in oocyte growth, oocyte maturation, and oocyte-to-embryo transition. J. Exp. Zool. 313A:461-473, 2010. (C) 2010 Wiley-Liss, Inc.
KeywordCarassius-auratus-gibelio Cortical Granules Mouse Oocytes Vasa Rna Protein Expression Gene Zebrafish Fish Identification
Department[Sun, Min; Li, Zhi; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaZoology
Funding OrganizationNational Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301] ; National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301] ; National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301] ; National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301]
Indexed BySCI
Language英语
WOS IDWOS:000282663100007
Funding OrganizationNational Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301] ; National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301] ; National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301] ; National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301]
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13581
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Sun, Min,Li, Zhi,Gui, Jian-Fang,et al. Dynamic Distribution of Spindlin in Nucleoli, Nucleoplasm and Spindle From Primary Oocytes to Mature Eggs and its Critical Function for Oocyte-to-Embryo Transition in Gibel Carp[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY,2010,313A(8):461-473.
APA Sun, Min,Li, Zhi,Gui, Jian-Fang,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.(2010).Dynamic Distribution of Spindlin in Nucleoli, Nucleoplasm and Spindle From Primary Oocytes to Mature Eggs and its Critical Function for Oocyte-to-Embryo Transition in Gibel Carp.JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY,313A(8),461-473.
MLA Sun, Min,et al."Dynamic Distribution of Spindlin in Nucleoli, Nucleoplasm and Spindle From Primary Oocytes to Mature Eggs and its Critical Function for Oocyte-to-Embryo Transition in Gibel Carp".JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY 313A.8(2010):461-473.
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