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学科主题: Zoology
题名: Dynamic Distribution of Spindlin in Nucleoli, Nucleoplasm and Spindle From Primary Oocytes to Mature Eggs and its Critical Function for Oocyte-to-Embryo Transition in Gibel Carp
作者: Sun, Min1; Li, Zhi1; Gui, Jian-Fang1
通讯作者: Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China
关键词: CARASSIUS-AURATUS-GIBELIO ; CORTICAL GRANULES ; MOUSE OOCYTES ; VASA RNA ; PROTEIN ; EXPRESSION ; GENE ; ZEBRAFISH ; FISH ; IDENTIFICATION
刊名: JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY
发表日期: 2010-10-01
DOI: 10.1002/jez.618
卷: 313A, 期:8, 页:461-473
收录类别: SCI
文章类型: Article
部门归属: [Sun, Min; Li, Zhi; Gui, Jian-Fang] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Key Basic Research Program [2010CB126301]; National Natural Science Foundation of China [30630050]; National Technology System for Conventional Freshwater Fish Industries, Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [075A011301, 085A021301]
类目[WOS]: Zoology
研究领域[WOS]: Zoology
摘要: Spindlin (Spin) was thought as a maternal-effect factor associated with meiotic spindle. Its role for the oocyte-to-embryo transition was suggested in mouse, but its direct evidence for the function had been not obtained in other vertebrates. In this study, we used the CagSpin-specific antibody to investigate CagSpin expression pattern and distribution during oogenesis of gibel carp (Carassius auratus gibelio). First, the oocyte-specific expression pattern and dynamic distribution was revealed in nucleoli, nucleoplasm, and spindle from primary oocytes to mature eggs by immunofluorescence localization. In primary oocytes and growth stage oocytes, CagSpin accumulates in nucleoli in increasing numbers along with the oocyte growth, and its disassembly occurs in vitellogenic oocytes, which implicates that CagSpin may be a major component of a large number of nucleoli in fish growth oocytes. Then, co-localization of CagSpin and beta-tubulin was revealed in meiotic spindle of mature egg, indicating that CagSpin is one spindle-associated factor. Moreover, microinjection of CagSpin-specific antibody into the fertilized eggs blocked the first cleavage, and found that the CagSpin depletion resulted in spindle assembly disturbance. Thereby, our study provided the first direct evidence for the critical oocyte-to-embryo transition function of Spin in vertebrates, and confirmed that Spin is one important maternal-effect factor that participates in oocyte growth, oocyte maturation, and oocyte-to-embryo transition. J. Exp. Zool. 313A:461-473, 2010. (C) 2010 Wiley-Liss, Inc.
英文摘要: Spindlin (Spin) was thought as a maternal-effect factor associated with meiotic spindle. Its role for the oocyte-to-embryo transition was suggested in mouse, but its direct evidence for the function had been not obtained in other vertebrates. In this study, we used the CagSpin-specific antibody to investigate CagSpin expression pattern and distribution during oogenesis of gibel carp (Carassius auratus gibelio). First, the oocyte-specific expression pattern and dynamic distribution was revealed in nucleoli, nucleoplasm, and spindle from primary oocytes to mature eggs by immunofluorescence localization. In primary oocytes and growth stage oocytes, CagSpin accumulates in nucleoli in increasing numbers along with the oocyte growth, and its disassembly occurs in vitellogenic oocytes, which implicates that CagSpin may be a major component of a large number of nucleoli in fish growth oocytes. Then, co-localization of CagSpin and beta-tubulin was revealed in meiotic spindle of mature egg, indicating that CagSpin is one spindle-associated factor. Moreover, microinjection of CagSpin-specific antibody into the fertilized eggs blocked the first cleavage, and found that the CagSpin depletion resulted in spindle assembly disturbance. Thereby, our study provided the first direct evidence for the critical oocyte-to-embryo transition function of Spin in vertebrates, and confirmed that Spin is one important maternal-effect factor that participates in oocyte growth, oocyte maturation, and oocyte-to-embryo transition. J. Exp. Zool. 313A:461-473, 2010. (C) 2010 Wiley-Liss, Inc.
关键词[WOS]: CARASSIUS-AURATUS-GIBELIO ; CORTICAL GRANULES ; MOUSE OOCYTES ; VASA RNA ; PROTEIN ; EXPRESSION ; GENE ; ZEBRAFISH ; FISH ; IDENTIFICATION
语种: 英语
WOS记录号: WOS:000282663100007
ISSN号: 1932-5223
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13581
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Sun, Min; Li, Zhi; Gui, Jian-Fang.Dynamic Distribution of Spindlin in Nucleoli, Nucleoplasm and Spindle From Primary Oocytes to Mature Eggs and its Critical Function for Oocyte-to-Embryo Transition in Gibel Carp,JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY,2010,313A(8):461-473
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