IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
EseG, an Effector of the Type III Secretion System of Edwardsiella tarda, Triggers Microtubule Destabilization
Xie, Hai Xia; Yu, Hong Bing; Zheng, Jun; Nie, Pin; Foster, Leonard J.; Mok, Yu-Keung; Finlay, B. Brett; Leung, Ka Yin; Leung, KY, Trinity Western Univ, Fac Nat & Appl Sci, Dept Biol, 7600 Glover Rd, Langley, BC V2Y 1Y1, Canada
2010
Source PublicationINFECTION AND IMMUNITY
ISSN0019-9567
Volume78Issue:12Pages:5011-5021
AbstractEdwardsiella tarda is a Gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and both gastrointestinal and extraintestinal infections in humans. A type III secretion system (T3SS) was recently shown to contribute to pathogenesis, since deletions of various T3SS genes increased the 50% lethal dose (LD50) by about 1 log unit in the blue gourami infection model. In this study, we report EseG as the first identified effector protein of T3SS. EseG shares partial homology with two Salmonella T3SS effectors (SseG and SseF) over a conserved domain (amino acid residues 142 to 192). The secretion of EseG is dependent on a functional T3SS and, in particular, requires the chaperone EscB. Experiments using TEM-1 beta-lactamase as a fluorescence-based reporter showed that EseG was translocated into HeLa cells at 35 degrees C. Fractionation of infected HeLa cells demonstrated that EseG was localized to the host membrane fraction after translocation. EseG is able to disassemble microtubule structures when overexpressed in mammalian cells. This phenotype may require a conserved motif of EseG (EseG(142-192)), since truncated versions of EseG devoid of this motif lose their ability to cause microtubule destabilization. By demonstrating the function of EseG, our study contributes to the understanding of E. tarda pathogenesis. Moreover, the approach established in this study to identify type III effectors can be used to identify and characterize more type III and possible type VI effectors in Edwardsiella.
KeywordEnteropathogenic Escherichia-coli
Department[Leung, Ka Yin] Trinity Western Univ, Fac Nat & Appl Sci, Dept Biol, Langley, BC V2Y 1Y1, Canada; [Xie, Hai Xia; Zheng, Jun; Mok, Yu-Keung; Leung, Ka Yin] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 117543, Singapore; [Xie, Hai Xia; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Yu, Hong Bing; Finlay, B. Brett] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada; [Foster, Leonard J.] Univ British Columbia, Ctr High Throughput Biol, Dept Biochem & Mol Biol, Vancouver, BC V6T 1Z4, Canada
Subject AreaImmunology ; Infectious Diseases
Funding OrganizationAgency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688] ; Agency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688] ; Agency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688] ; Agency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688]
Indexed BySCI
Language英语
WOS IDWOS:000284213600005
Funding OrganizationAgency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688] ; Agency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688] ; Agency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688] ; Agency for Science, Technology, and Research (A*STAR), Singapore [04/1/21/19/346, 06/1/21/16/431, 07/1/21/19/495]; Natural Science and Engineering Research Council (NSERC) [372373-2010]; Trinity Western startup grant [0488]; Canadian Institutes of Health Research (CIHR) [MOP-77688]
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13573
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLeung, KY, Trinity Western Univ, Fac Nat & Appl Sci, Dept Biol, 7600 Glover Rd, Langley, BC V2Y 1Y1, Canada
Recommended Citation
GB/T 7714
Xie, Hai Xia,Yu, Hong Bing,Zheng, Jun,et al. EseG, an Effector of the Type III Secretion System of Edwardsiella tarda, Triggers Microtubule Destabilization[J]. INFECTION AND IMMUNITY,2010,78(12):5011-5021.
APA Xie, Hai Xia.,Yu, Hong Bing.,Zheng, Jun.,Nie, Pin.,Foster, Leonard J..,...&Leung, KY, Trinity Western Univ, Fac Nat & Appl Sci, Dept Biol, 7600 Glover Rd, Langley, BC V2Y 1Y1, Canada.(2010).EseG, an Effector of the Type III Secretion System of Edwardsiella tarda, Triggers Microtubule Destabilization.INFECTION AND IMMUNITY,78(12),5011-5021.
MLA Xie, Hai Xia,et al."EseG, an Effector of the Type III Secretion System of Edwardsiella tarda, Triggers Microtubule Destabilization".INFECTION AND IMMUNITY 78.12(2010):5011-5021.
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