IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Characterization of Fish IRF3 as an IFN-Inducible Protein Reveals Evolving Regulation of IFN Response in Vertebrates
Sun, Fan; Zhang, Yi-Bing; Liu, Ting-Kai; Gan, Li; Yu, Fei-Fei; Liu, Ying; Gui, Jian-Fang; Zhang, YB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
2010
Source PublicationJOURNAL OF IMMUNOLOGY
ISSN0022-1767
Volume185Issue:12Pages:7573-7582
AbstractIn mammals, IFN regulatory factor (IRF) 3 is a critical player in modulating transcription of type I IFN and IFN-stimulated genes (ISGs). In this study, we describe the roles of crucian carp (Carassius auratus L.) IRF3 in activating fish IFN and ISGs. Fish IRF3 exhibits a large sequence divergence from mammalian orthologs. Whereas mammalian IRF3 is constitutively expressed, fish IRF3 protein is significantly upregulated by IFN, poly-IC, and other stimuli known as IFN inducers in mammals. The IFN-inducible property of fish IRF3 is consistent with the comparative analysis of 5' flanking regulatory region of vertebrate IRF3 genes, which reveals the presence of typical IFN-stimulated response elements in fish and amphibians, but an absence in tetrapods. Furthermore, either IFN or poly-IC induces phosphorylation and cytoplasmic-to-nuclear translocation of IRF3, which seems essential for its function in that phosphomimic active IRF3 exhibits stronger transactivation than wild type IRF3. Finally, overexpression of fish IRF3 activates production of IFN that in turn triggers ISG transcription through Stat1 pathway, whereas transfection of dominant negative mutant IRF3-DN abrogates poly-IC induction of ISGs, probably owing to blockade of IFN production. Therefore, regulation of IFN response by vertebrate IRF3 is another ancient trait. These data provide evidence of the evolving function of vertebrate IRF3 on regulating IFN response. The Journal of Immunology, 2010, 185: 7573-7582.
KeywordDouble-stranded-rna
Department[Sun, Fan; Zhang, Yi-Bing; Liu, Ting-Kai; Gan, Li; Yu, Fei-Fei; Liu, Ying; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
Subject AreaImmunology
Funding Organization973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01] ; 973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01] ; 973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01] ; 973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01]
Indexed BySCI
Language英语
WOS IDWOS:000284878700056
Funding Organization973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01] ; 973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01] ; 973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01] ; 973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30871922]; National Transgenic Project [2009ZX08010-021B]; Freshwater Ecology and Biotechnology Laboratory [2009FBZ01]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13567
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, YB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Sun, Fan,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Characterization of Fish IRF3 as an IFN-Inducible Protein Reveals Evolving Regulation of IFN Response in Vertebrates[J]. JOURNAL OF IMMUNOLOGY,2010,185(12):7573-7582.
APA Sun, Fan.,Zhang, Yi-Bing.,Liu, Ting-Kai.,Gan, Li.,Yu, Fei-Fei.,...&Zhang, YB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Grad Sch, Wuhan 430072, Peoples R China.(2010).Characterization of Fish IRF3 as an IFN-Inducible Protein Reveals Evolving Regulation of IFN Response in Vertebrates.JOURNAL OF IMMUNOLOGY,185(12),7573-7582.
MLA Sun, Fan,et al."Characterization of Fish IRF3 as an IFN-Inducible Protein Reveals Evolving Regulation of IFN Response in Vertebrates".JOURNAL OF IMMUNOLOGY 185.12(2010):7573-7582.
Files in This Item:
File Name/Size DocType Version Access License
Characterization of (2823KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Fan]'s Articles
[Zhang, Yi-Bing]'s Articles
[Liu, Ting-Kai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Fan]'s Articles
[Zhang, Yi-Bing]'s Articles
[Liu, Ting-Kai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Fan]'s Articles
[Zhang, Yi-Bing]'s Articles
[Liu, Ting-Kai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Characterization of Fish IRF3 as an IFN-Inducible Protein Reveals Evolving Regulation of IFN Response in Vertebrates.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.