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Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of microcystin-RR and its metabolites in fish liver
Wu, Laiyan; Xie, Ping; Chen, Jun; Zhang, Dawen; Liang, Gaodao; Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
2010
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1217Issue:9Pages:1455-1462
AbstractA novel method for identification and quantification of microcystin-RR (MC-RR) and its metabolites (MC-RR-GSH and MC-RR-Cys) in the fish liver was developed and validated. These analytes were simultaneously extracted from fish liver using water containing EDTA with 5% acetic acid, followed by a mixed-mode cation-exchange SPE (Oasis MCX) and subsequently determined by liquid chromatography-electrospray ionization ion trap mass spectrometry (LC-ESI-ITMS). Extraction parameters including volume and pH of eluting solvents, were optimized. Best recoveries were obtained by using 10 mL of 15% ammonia solution in methanol. The mean recoveries at three concentrations (0.2, 1.0, and 5.0 mu g g(-1) dry weight [DW]) for MC-RR, MC-RR-GSH and MC-RR-Cys were 93.6-99%,68.1-73.6% and 90.0-95.2%, respectively. Method detection limit (MDL) were 4, 7 and 5 ng g(-1) DW for MC-RR, MC-RR-GSH and MC-RR-Cys, respectively. Limits of quantification (LOQs) for MC-RR, MC-RR-GSH and MC-RR-Cys were calculated to be 10, 18 and 13 ng g(-1) DW, respectively. Finally, this method was successfully applied to the identification and quantification of MC-RR, MC-RR-GSH and MC-RR-Cys in the liver of bighead carp with acute exposure of MCs. (C) 2009 Elsevier B.V. All rights reserved.
KeywordMicrocystin Lc-ms/ms Spe Method Validation
Department[Wu, Laiyan; Xie, Ping; Chen, Jun; Zhang, Dawen; Liang, Gaodao] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationChinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]
Indexed BySCI
Language英语
WOS IDWOS:000274953500005
Funding OrganizationChinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02] ; Chinese Academy of Sciences [KZCX1-YW-14-6-1]; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]
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Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13550
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wu, Laiyan,Xie, Ping,Chen, Jun,et al. Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of microcystin-RR and its metabolites in fish liver[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(9):1455-1462.
APA Wu, Laiyan,Xie, Ping,Chen, Jun,Zhang, Dawen,Liang, Gaodao,&Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Peoples R China.(2010).Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of microcystin-RR and its metabolites in fish liver.JOURNAL OF CHROMATOGRAPHY A,1217(9),1455-1462.
MLA Wu, Laiyan,et al."Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of microcystin-RR and its metabolites in fish liver".JOURNAL OF CHROMATOGRAPHY A 1217.9(2010):1455-1462.
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