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Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)
Li, Guangyu; Xie, Ping; Li, Huiying; Chen, Jun; Hao, Le; Xiong, Qian; Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2010
Source PublicationJOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
ISSN1095-6670
Volume24Issue:4Pages:250-259
AbstractThe expression of glutathione S-transferase (GST) is a crucial factor in determining the sensitivity of cells and organs in response to a variety of toxicants. In this study, we cloned the core nucleotide of alpha, kappa, mu, mGST, pi, rho, and theta-like GST genes from bighead carp (Aristichthys nobilis). Their derived amino acid sequences were clustered with other vertebrate GSTs in a phylogenetic tree, and the bighead carp GST sequences have the highest similarity with those from common carp and zebrafish. We quantified the constitutive mRNA transcription of GST isoforms in eight different tissues (liver, kidney, spleen, intestine, muscle, heart, brain, and gill). The information obtained from the present study could be distilled into a few generalized principles: multiple GST isoenzymes were ubiquitously expressed in all tissues; majority of GSTs had high constitutive expression in intestine, liver, and kidney. These findings are in agreement with the roles of these tissues in xenobiotic metabolism. At the same time, some unique findings were detected in the current experiment: (1) higher expression of most GSTs was observed in spleen; (2) the expression of GST pi was highest in almost all the studied tissues except muscle; the other two isoforms, GST alpha and rho, were also highly expressed in liver, kidney, intestine, spleen, heart, and brain of bighead carp. All these results strongly imply an important role of these GST isoforms in detoxification of ingested xenobiotics. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:250-259, 2010; View this article online at wileyonlinelibrary.com. DOI10:1002/jbt.20333
KeywordGlutathione S-transferases Tissue Distribution Real-time Pcr Gene Expression Aristichthys Nobilis
Department[Li, Guangyu; Xie, Ping; Li, Huiying; Chen, Jun; Hao, Le; Xiong, Qian] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Toxicology
Funding OrganizationMinistry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] ; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] ; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] ; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480]
Indexed BySCI
Language英语
WOS IDWOS:000281455800007
Funding OrganizationMinistry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] ; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] ; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480] ; Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/13522
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Guangyu,Xie, Ping,Li, Huiying,et al. Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2010,24(4):250-259.
APA Li, Guangyu.,Xie, Ping.,Li, Huiying.,Chen, Jun.,Hao, Le.,...&Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2010).Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis).JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,24(4),250-259.
MLA Li, Guangyu,et al."Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 24.4(2010):250-259.
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