中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
学科主题: Biochemistry & Molecular Biology; Toxicology
题名: Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis)
作者: Li, Guangyu1; Xie, Ping1; Li, Huiying1; Chen, Jun1; Hao, Le1; Xiong, Qian1
通讯作者: Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: Glutathione S-Transferases ; Tissue Distribution ; Real-Time PCR ; Gene Expression ; Aristichthys nobilis
刊名: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
发表日期: 2010
DOI: 10.1002/jbt.20333
卷: 24, 期:4, 页:250-259
收录类别: SCI
文章类型: Article
部门归属: [Li, Guangyu; Xie, Ping; Li, Huiying; Chen, Jun; Hao, Le; Xiong, Qian] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Ministry of Science and Technology of the People's Republic of China [2007BAC26B02]; National Natural Science Foundation of China [30700077]; NSFC-RSE [30710480]
类目[WOS]: Biochemistry & Molecular Biology ; Toxicology
研究领域[WOS]: Biochemistry & Molecular Biology ; Toxicology
摘要: The expression of glutathione S-transferase (GST) is a crucial factor in determining the sensitivity of cells and organs in response to a variety of toxicants. In this study, we cloned the core nucleotide of alpha, kappa, mu, mGST, pi, rho, and theta-like GST genes from bighead carp (Aristichthys nobilis). Their derived amino acid sequences were clustered with other vertebrate GSTs in a phylogenetic tree, and the bighead carp GST sequences have the highest similarity with those from common carp and zebrafish. We quantified the constitutive mRNA transcription of GST isoforms in eight different tissues (liver, kidney, spleen, intestine, muscle, heart, brain, and gill). The information obtained from the present study could be distilled into a few generalized principles: multiple GST isoenzymes were ubiquitously expressed in all tissues; majority of GSTs had high constitutive expression in intestine, liver, and kidney. These findings are in agreement with the roles of these tissues in xenobiotic metabolism. At the same time, some unique findings were detected in the current experiment: (1) higher expression of most GSTs was observed in spleen; (2) the expression of GST pi was highest in almost all the studied tissues except muscle; the other two isoforms, GST alpha and rho, were also highly expressed in liver, kidney, intestine, spleen, heart, and brain of bighead carp. All these results strongly imply an important role of these GST isoforms in detoxification of ingested xenobiotics. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:250-259, 2010; View this article online at wileyonlinelibrary.com. DOI10:1002/jbt.20333
英文摘要: The expression of glutathione S-transferase (GST) is a crucial factor in determining the sensitivity of cells and organs in response to a variety of toxicants. In this study, we cloned the core nucleotide of alpha, kappa, mu, mGST, pi, rho, and theta-like GST genes from bighead carp (Aristichthys nobilis). Their derived amino acid sequences were clustered with other vertebrate GSTs in a phylogenetic tree, and the bighead carp GST sequences have the highest similarity with those from common carp and zebrafish. We quantified the constitutive mRNA transcription of GST isoforms in eight different tissues (liver, kidney, spleen, intestine, muscle, heart, brain, and gill). The information obtained from the present study could be distilled into a few generalized principles: multiple GST isoenzymes were ubiquitously expressed in all tissues; majority of GSTs had high constitutive expression in intestine, liver, and kidney. These findings are in agreement with the roles of these tissues in xenobiotic metabolism. At the same time, some unique findings were detected in the current experiment: (1) higher expression of most GSTs was observed in spleen; (2) the expression of GST pi was highest in almost all the studied tissues except muscle; the other two isoforms, GST alpha and rho, were also highly expressed in liver, kidney, intestine, spleen, heart, and brain of bighead carp. All these results strongly imply an important role of these GST isoforms in detoxification of ingested xenobiotics. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:250-259, 2010; View this article online at wileyonlinelibrary.com. DOI10:1002/jbt.20333
关键词[WOS]: PLAICE PLEURONECTES-PLATESSA ; OXIDATIVE STRESS ; CLASS-KAPPA ; FISH ; DETOXIFICATION ; BIOMARKERS ; MOUSE ; SUSCEPTIBILITY ; IDENTIFICATION ; ISOENZYMES
语种: 英语
WOS记录号: WOS:000281455800007
ISSN号: 1095-6670
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/13522
Appears in Collections:淡水生态学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis).pdf(164KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Li, Guangyu; Xie, Ping; Li, Huiying; Chen, Jun; Hao, Le; Xiong, Qian.Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis),JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2010,24(4):250-259
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Guangyu]'s Articles
[Xie, Ping]'s Articles
[Li, Huiying]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Guangyu]‘s Articles
[Xie, Ping]‘s Articles
[Li, Huiying]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Quantitative Profiling of mRNA Expression of Glutathione S-transferase Superfamily Genes in Various Tissues of Bighead Carp (Aristichthys nobilis).pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace