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诱变筛选鱼类HGPRT缺陷型细胞的初步试探
甘新生
Subtype硕士
Thesis Advisor陆仁后
1990
Degree Grantor中国科学院水生生物研究所·武汉
Place of Conferral中国科学院水生生物研究所·武汉
Degree Discipline遗传学
Keyword草鱼卵巢细胞 诱变 6-巯基嘌吟 Hrprt缺陷型
Abstract草鱼卵巢细胞GCG经乙基甲磺酸(EMS)诱变,稳定表达3代以后,采取逐步提高培养基中6-巯基嘌呤(6-MP)的方法,获得对6-MP稳定抗性的细胞,暂定名FMR-1。本文比较了GCG细胞及FMR-1细胞在含6-MP(20μg/ml)和不含6-MP的培养基中生长曲线的特点,并对上述两种细胞的染色体数目、乳酸脱氢酶及葡萄糖-6-磷酸脱氢酶图谱进行了比较分析,最后对两种细胞在HAT培养基中的生长特点进行了平行对照实验,根据实验结果,可以初步判定FMR-1为草鱼HGPRT缺陷型细胞。本文还讨论了进一步研究草鱼HGPRT缺陷型细胞的途径及草鱼HGPRT缺陷型细胞在鱼类细胞遗传学的研究中可能的应用前景。
Other AbstractThe cell line GCG originated from o Vary of grass carp (Ctenpharyngodon idellus Cur. et Val.) was treated with ethyl methane sulfonate (EMS) for 72 hours. After stable expression of mutation for 3 passages, the cells were treated with 6-mercaptopurine (6-MP) from 4μg/ml to 100μg/ml by stepwise procedures. HaVing examined growth of the isolates in HAT mediums, we obtained mutants named FMR-1 which are deficient in hypoxanthine guanine phosphoribosyl transferase (HGPRT). Identification of biological characteristic of FMR-1 cells showed that chromosomes dec reased in number compared with those of the parental GCG cells. Growth curves of FMR-1 and GCG in TC-100 mediums containing 6-MP (20μg/ml) and no 6-MP were also illustrated. The analysis of LDH and G-6-PD isoenzymes of FMR-1 cells displayed almost the same patterns as those of GCG cells. Further measures to study on the fish HGPRT deficient cells and possible applications of these cells were discussed.
Pages34
Language中文
Document Type学位论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/12814
Collection学位论文
Recommended Citation
GB/T 7714
甘新生. 诱变筛选鱼类HGPRT缺陷型细胞的初步试探[D]. 中国科学院水生生物研究所·武汉. 中国科学院水生生物研究所·武汉,1990.
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