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Thesis Advisor陆仁后
Degree Grantor中国科学院水生生物研究所·武汉
Place of Conferral中国科学院水生生物研究所·武汉
Degree Discipline遗传学
Keyword草鱼卵巢细胞 诱变 6-巯基嘌吟 Hrprt缺陷型
Other AbstractThe cell line GCG originated from o Vary of grass carp (Ctenpharyngodon idellus Cur. et Val.) was treated with ethyl methane sulfonate (EMS) for 72 hours. After stable expression of mutation for 3 passages, the cells were treated with 6-mercaptopurine (6-MP) from 4μg/ml to 100μg/ml by stepwise procedures. HaVing examined growth of the isolates in HAT mediums, we obtained mutants named FMR-1 which are deficient in hypoxanthine guanine phosphoribosyl transferase (HGPRT). Identification of biological characteristic of FMR-1 cells showed that chromosomes dec reased in number compared with those of the parental GCG cells. Growth curves of FMR-1 and GCG in TC-100 mediums containing 6-MP (20μg/ml) and no 6-MP were also illustrated. The analysis of LDH and G-6-PD isoenzymes of FMR-1 cells displayed almost the same patterns as those of GCG cells. Further measures to study on the fish HGPRT deficient cells and possible applications of these cells were discussed.
Document Type学位论文
Recommended Citation
GB/T 7714
甘新生. 诱变筛选鱼类HGPRT缺陷型细胞的初步试探[D]. 中国科学院水生生物研究所·武汉. 中国科学院水生生物研究所·武汉,1990.
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诱变筛选鱼类HGPRT缺陷型细胞的初步试(1458KB) 限制开放--Application Full Text
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