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草鱼(Ctenopharyngodon idellns C.et.V)鳃细胞系GCG的建立及细胞分裂控制机制的研究
Thesis Advisor倪达书
Degree Grantor中国科学院水生生物研究所
Place of Conferral中国科学院水生生物研究所
Degree Discipline水生生物学
Abstract本文作者用含10%胎牛血清的199培养液在25 ℃建立了来源于草鱼鳃组织的成纤维细胞系GCG。GCG细胞在12天的培养期内出现了三个指数生长期,在第一个指数生长期的倍增时间为51小时。该细胞系的染色体数为46,最低接种密度为15,000细胞/ml,克隆率为3%,温度适应范围为15-34 ℃。GCG细胞对草鱼弹状病毒敏感,其正常的生长要求10%的胎牛血清。目前,该细胞系已传至90余代。根据GCG细胞的生长特性及其它性质,本文提出了一个关于离体细胞分裂控制的假说:1、细胞分裂是由一对作用相反的细胞因子-细胞分裂抑制因子(MIF)和细胞分裂释放因子(MRF)-控制的;2、MIF在某一浓度阈抑制细胞分裂并受反馈机制控制;3、任一给定细胞系合成MIF和MRF的量是其本身固有的性质。本文还用此假说解释了正常细胞及恶性细胞的分裂行为。
Other AbstractA fibroblastic cell line GCG derived from grass xarp gill was established by using 199 medium plus 10% feral calf serum at 25℃. The cell lime had three exponential growth phases over an incubation period of 12 days with the doubling time of 51 hours in the first phase. Its chromosomal model number was 46, minimal plating dense, 15,000 cells/ml, and cloning efficency, 3%. 10% fetal calf serum was required for its normal growth. The cell line was sensitive to grass carp rhabdvirus. It has been subcultured for more than 90 passages since its initiation in November 1987. Based on the unique properties of the growth curve and additional data, a hypothesis concerning the control of the division of cells in vitro was ptoposed. It was advaneed that (1)cell division was controled by a pair of contraacting cellular factors-Mitosis Inhibition Factor(MIF) and Miosis Relase Factor(MRF); (2) MIF inhibited cell division at a certian concetration threshold and was monitored by feedback control; (3) the quantities of MIF and MRF that a given cell line synthesized were characteristic of the cell. The growth behavior of normal and malignant cells in vitro were explained by using this theory.
Document Type学位论文
Recommended Citation
GB/T 7714
刘至东. 草鱼(Ctenopharyngodon idellns C.et.V)鳃细胞系GCG的建立及细胞分裂控制机制的研究[D]. 中国科学院水生生物研究所. 中国科学院水生生物研究所,1989.
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