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丝状蓝藻Anabaena7120野生种与防氧突变株的质膜、类囊体膜和细胞壁的分离及比较分析
杨劭
Subtype硕士
Thesis Advisor王业勤 ; 黎尚豪
1989
Degree Grantor中国科学院水生生物研究所
Place of Conferral中国科学院水生生物研究所
Degree Discipline水生生物学
Keyword细胞壁 质膜 类囊体膜 分离
Abstract本文采用机械性方法破碎细胞,再以非连续蔗糖密度梯度离心法分离并获得了丝状蓝藻Anabaena 7120及其氧敏感突变株Anabaena-1营养细胞的纯质膜、类囊体膜和有类囊体膜污染的细胞壁;以Triton处理法获得纯细胞壁。制备的质膜在455、482、519和673 nm处有光谱吸收,其SDS-PAGE图谱显示30多条蛋白带并有48、55和104KD三条主要蛋白带,类囊体体膜在419、438、490和679nm处有光谱吸收,其SDS-PAGE图谱显示40多条蛋白带并有一条54KD主要蛋白带,细胞质可溶性物质的SDS-PAGE图谱显示30多条蛋白带,其中藻胆蛋白含量最大;Triton不溶细胞壁的SDS-PAGE图谱显示40多条蛋白带,其中的主要蛋白带52KD为跨膜蛋白并与肽聚糖层以非共价键相连;细胞壁大部分蛋白质对胰蛋白酶处理敏感;52KD和14KD为糖蛋白,其上没有发现“热修饰”蛋白。另外文章还比较分析了丝状蓝藻和单细胞蓝藻质膜、类囊体膜和细胞壁性质的差别,以及野生型Anabaena 7120和突变株Anabaena-1之间生理和质膜、类囊体膜、细胞壁性质的差异。
Other AbstractPure cytoplasmic, thylakoid membranes and cell walls which were contaminated by thylakoid of the filamentous cyanobacteria Anabaena 7120 and its oxygen-sensitive mutant Anabaena-1 were isolated by mechanical disruption of the vegetative cells followed by discontinuous sucrose density gradient centrifugation. Pure cell walls were obtained from crude membranes by extraction with 2% Ttiton X-100. The prepared cytoplasmic membrane had spectral absorption at 455, 482, 519 and 673nm. Its SDS-PAGE map showed more than 30 polypeptide bands, among which there were three major bands which had molecular weight of 48, 55 and 104KD. The prepared thylakoid membrane had spectral absorption at 419, 438, 490 and 679nm. Its SDS-PAGE map showed more than 40 polypeptide bands, among them there was one major band of 54KD. The SDS-PAGE map of the cytoplasmic soluble fraction had more than 30 polypeptide bands, the amount of phycobitiproteins was the most of them. The SDS-PAGE map of the Triton-insoluble cell wall showed more than 40 polypeptide bands. The major polypeptide band(52KD) was mosaic protein and associated with the peptidoglycan non-covalently. Most of the proteins of the cell wall was sensitive to trypsin. The polypeptide of 52KD and 14KD were glycoproteins. No "heat-modifiable protein" was discovered on the cell walls of these two filamentous algae. This article compared the characteristics of the cytoplasmic, thylakoid membranes and the cell walls of the filamentous and unicellular algae. The differences of some physiological characters and characteristics of cytoplasmic, thylakoid membranes, cell walls between the wild type Anabaena7120 and the mutant Anabaena-1 were also discussed here.
Pages34
Language中文
Document Type学位论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/12778
Collection学位论文
Recommended Citation
GB/T 7714
杨劭. 丝状蓝藻Anabaena7120野生种与防氧突变株的质膜、类囊体膜和细胞壁的分离及比较分析[D]. 中国科学院水生生物研究所. 中国科学院水生生物研究所,1989.
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