The south-east Guizhou is located on the south-east boundary of Yunnan-guizhou plateau. Its fish-fauna is of significance on studies of the geographic distribution, the history of envolvement and the geographic division of the ichthyofauna of Yunnan-guizhou plateau. The auther made a large fish collection from the south-east Guizhou in April---June 1987, 106 species were identified, among which Noemacheilus liboensis, Sinocyclocheilus macrolepis, Sinocyclocheilus longibarbatus, Sinocrossocheilus microstomatus and Beaufortia intermedia were proved to be new to science. The ichthyofauna of the south-east Guizhou is composed of the fish components of Zhujiang River, changjiang River and Yunnan-guizhou Plateau. Namely, the ichthyofauna of Dagouthe River belongs to Yunnanguizhou Plateau, that of Duliujiang River to Zhujiang River, and those of Wujiang RIver and Yuanjiang River to Changjiang River, The formation of the ichthyofauna in this area is mainly related to the uplift of Qinghai-xizang Plateau. During the Tertiary Period, such primitive groups of Barbinae and Cobitidae as Sinocyclocheilus, Barbodes and Yunnanalis might have occured in the area of the present Yunnan-guizhou Plateau. Consequently quently, the rapid uplift of Yunnan-guizhou Plateau accompanying with Qinghai-xizang Plateau beginning from Olingocene Epoch resulted in intense change of the geomorphic and climatic conditions in the area, which had profound influence on the envolvement of its fish-fauna in two aspects: 1) the formation of new groups (such as Sinocyclocheilus, Crossocheilus, Sinocrossocheilus and some species of Schitherax) from the primitive Barbinae; 2) the species differentiation of the early Tertiary fish groups (such as Barbodes, Yunnanalis). All these groups constituted the special components of the ichthyofauna of Yunnan-guizhou Plateau, and their distribution range could be considered as the evidence of establishing the realm of the Yunnan-guizhou Plateau ichthyofauna. It is suggested that Yunnan-guizhou Plateau should be determined as an integral ichthyogeographic division unit included in the Oriental Region.