In this paper, blastula cells of Paramisgurnus dabryanus were fused with eggs of the same species b by the electrofusion technique. The cell-fused eggs can cleave and develop and finally give birth fires that can grow up to adult fish. The first several cleavages of cell-fused egg are different from that of fertilized egg, blastomeres are disparity in size and irregular in order, some times there are more than two blastomers emerging in the first cleavage, the number of blastomers can be odd. These deformitive embryos can be gradually corrected during the following development, by the time of blastula stage, part of embryos had been corrected to normal and can develop continuously and give birth fries while the abnormal embryos would soon dead. The main experimental conditions of loach's blastula cells-unfertilized eggs electrofution had been studied emphatically in this paper, the results indicated that the concentration of Ca~(2+), mannitol and PronaseE, the types of various divalent cations, the density of donor cells, the quality of recipient eggs and the excitative extent of recipient eggs due to being contacted with water are the primary chemical and biological factors that influence the efficiency of electrofusion. The effect of Ca~(2+) on the electrofusion is the best among the four types of divalent cation: Ca~(2+), Mg~(2+), Mn~(2+), Co~(2+), while the concentration of Ca~(2+)and mannitol are 10~(-3) M and 0.2M respectively, the conductance and osmotic pressure of fusion medium are of best benefit to the rates of fusion and incubation. Pretreated donor cells with 100 Mg/ml PronaseE for 6-10 mim at 25 ℃, th fusion rate is enhanced markedly. Concering the biological factors, fusion rate enhanced markedly with the increasing of donor cells density while the density below 10~5 cells/ml. The possibility of incubation of fry is Little when the quality of recipent eggs is bad. When the loach eggs were contacted with water, the cytoplasm of egg excited immediately, higher the excitative extent, lower the hatching rate, so we accomplished fusion within 10 min after the eggs being contacted with water and obtained the satisfactory hatching rate. By improving the experimental conditions mentioned above, the fusion and hatching rate could reach 80% and 20% respectively in our experiments. Cytological studies on the early development of recipient eggs after cell fusion by the technique of parafin section were carried out. It was observed that after fusion most of the recipient eggs received 2-4 donor cell nuclei which were uncertainly located in the recipient egg cytoplasm. These donor nuclei could develop into cleavage spindles independently 50 min after fusion. Because each spindle acts as a division center and brings about the division of cytoplasm, several blastomeres appeared simutaneously at the first cleavage. It is still not clear; at present whether the development of cell-fused embryos is multinuclear or mononuclear origin, and it is necessary to carry out further investigation to draw conclusion. In additon the intergeneric electrofusions were also carried out with the recipient eggs from Paramisgurnus dabryanus and the donor cells from the hybrid embryos between Paramisgurnus dabryanys () and Misgurnus anguillicandatus (). The chromosome analysis at mid-gastrula showed that the chromosome number is the same as that of donor cells. The characters of the adult electofusion fish are identical with that of donor fish. These results indicate that electrofusion fish does develop from donor cell nucleus.