The green colonial alga Botryococcus braunii is characterized by its unusual high hydrocarbon contants. In general, however, the growth of this alga is slow, which is one of the difficulties of large-scale cultivation of this alga. To solve this problem, we started with various media. The experiments showed that the growth of B.braunii (Ab-3) was promoted in HB-119 medium (4-fold strength), and Chu13(4-fold strength) in presence of NaF, KH_2AsO_4 and glucose. The maximum growth rate in Chu13 * 4 medium was 0.89 and the biomass doubling time was 1.14 days. But in presence of NaF (0.3 mg. l~(-1). 1.14 and 0.89 days were obtained respectively. Adding NaF and glucose to the culture were not affected the production and component of hydrocarbons. But in presence of KH_2AsO_4 and HB-119 * 4 medium the production of hydrocarbons decreased. The change of the tolal sugar contants and O_2 evolution in algae in media Chu 13 * 4 and HB-119 *4 were in the same tendency during the earlier period of batch culture, but in HB-119 * 4 medium, O_2 evolution still increased continuously afterward. Hydrocarbons were detected by GC-MS. they all belonged to CnH2n-2 and CnH2n-4 series. The fractions of hydrocarbon from eleven various cultural conditions were determined gravimetrically. Two media Chu13 in presence of NaF(0.3mg.L~(-1)) and HB-119 were fited for isolation. Five unialgal strains were obtained. Two types of reproduction of B.braunii, fragmention and autospore, were observed. The effect of NaF on algal growth and the transformation from sugar to hydrocarbon were discussed. The posibility of large-scale cultivation of B.braunii as a renewable source of fule in the near future also considered.