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葡萄藻(Botryococcus braunii kutzing)的生长、发育及培养
Thesis Advisor俞敏娟
Degree Grantor中国科学院水生生物研究所
Place of Conferral中国科学院水生生物研究所
Degree Discipline水生生物学
Abstract葡萄藻(绿藻)含较高量的独特的碳氢化合物,但一般生长缓慢是大量培养的障碍之一。本实验从改变培养基入手以解决生长速度缓慢的问题,试验结果表明加入NaF(0.1-0.3 mg·l~(-1)), KH_2AsO_4 (0.5 mg·l~(-1)), 葡萄糖(265mg·l~(-1))于四倍的Chu13培养基中的四倍浓度的HB-119培养基这四种培养基均能促进Botryococcus braunii (Ab-3)的生长。在Chu13四倍的培养基中B. braunii(Ab-3)的最大生长率为0.89;最短增代时间为1.14天。而加入NaF后,最大生长率是1.14;最短增代时间为0.89天。NaF和葡萄糖不影响B.braunii(Ab-3)的碳氢化合物的含量和类型。Chu 10 * 4 和HB-119 * 4 两种培基中生长的B.braunii (Ab-3),其糖量和光合放氧量在培养的前期变化趋势是一致的,只是生长后期,在HB-119培养基中光合放氧量继续增加。GC-MS分析了四种不同培养条件下的Btryococcus braunii (Ab-3)的碳氢化合物类型及相对今是。均属CnH_2n-2, CnH_2n-4系列。测定了十一种不同培养条件下生长的B.braunii (Ab-3)的碳氢化合物的含量。Ch13(F)和HB-119, 二种培养基适合分离培养B.braunii (YNb-1)。已获得五个单种B.braunii (YNb-1)藻种。B.braunii (YNb-1)的生长,增殖方式有两种:群体断裂和似亲孢子。本文就明显促进B.braunii (Ab-3)生长的因子(如,氟化钠)进行了初步的讨论并讨论了葡萄藻与石油形成的关系及大规模培养葡萄藻作为燃料的可能性。
Other AbstractThe green colonial alga Botryococcus braunii is characterized by its unusual high hydrocarbon contants. In general, however, the growth of this alga is slow, which is one of the difficulties of large-scale cultivation of this alga. To solve this problem, we started with various media. The experiments showed that the growth of B.braunii (Ab-3) was promoted in HB-119 medium (4-fold strength), and Chu13(4-fold strength) in presence of NaF, KH_2AsO_4 and glucose. The maximum growth rate in Chu13 * 4 medium was 0.89 and the biomass doubling time was 1.14 days. But in presence of NaF (0.3 mg. l~(-1). 1.14 and 0.89 days were obtained respectively. Adding NaF and glucose to the culture were not affected the production and component of hydrocarbons. But in presence of KH_2AsO_4 and HB-119 * 4 medium the production of hydrocarbons decreased. The change of the tolal sugar contants and O_2 evolution in algae in media Chu 13 * 4 and HB-119 *4 were in the same tendency during the earlier period of batch culture, but in HB-119 * 4 medium, O_2 evolution still increased continuously afterward. Hydrocarbons were detected by GC-MS. they all belonged to CnH2n-2 and CnH2n-4 series. The fractions of hydrocarbon from eleven various cultural conditions were determined gravimetrically. Two media Chu13 in presence of NaF(0.3mg.L~(-1)) and HB-119 were fited for isolation. Five unialgal strains were obtained. Two types of reproduction of B.braunii, fragmention and autospore, were observed. The effect of NaF on algal growth and the transformation from sugar to hydrocarbon were discussed. The posibility of large-scale cultivation of B.braunii as a renewable source of fule in the near future also considered.
Document Type学位论文
Recommended Citation
GB/T 7714
许常虹. 葡萄藻(Botryococcus braunii kutzing)的生长、发育及培养[D]. 中国科学院水生生物研究所. 中国科学院水生生物研究所,1987.
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