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Thesis Advisor俞敏娟
Degree Grantor中国科学院水生生物研究所
Place of Conferral中国科学院水生生物研究所
Degree Discipline水生生物学
Abstract本文实验以巢湖微囊藻(Microcystis)水花为材料,采用昆明鼠腹腔注射法对其毒性进行了测试,发现在86年9-11月期间,仅9月8日采自塘西的铜绿微囊藻(M.aeruginosa)水花和9月9日采自忠庙的水花微囊藻(M.flos-aquae)水花是有毒的。铜绿微囊藻(M.aeruginosa)水花的最低致死剂量(LDmin)为90mg干藻/kg鼠重,水花微囊藻(M.aeruginosa)的LDmin大约为1000 mg 干藻/kg鼠重。当注射剂量超过LDmin时,受试动物均在4小时死亡,其明显症状是引起肝脏淤血肿大。通过对铜绿微囊藻(M.aeruginosa)毒素理化性质的研究,发现其毒素是热稳定性的。毒素经甲醇/正丁醇抽提、C18吸附、离子交换层析和高压液相色谱等分离纯化后,毒性的回收率为16.36%,分离纯化所得毒素的纯度达95.75%。毒素在240nm有一强烈吸收峰。毒素经酸水解后,测得其氨基酸组成的, Asp(1), Clu(3), Ala(3), Leu(3), Arg(2)。并对其结构进行了初步分析。在对巢湖铜绿微囊藻(M.aeruginosa)毒素进行定量研究时,以M.aeruginosa 7820纯毒素为标准毒素,测得巢湖铜绿微囊藻(M.aeruginosa)水花毒素含量相当于4.45mg 7820毒素/g干藻,纯毒素的LDmin为0.33mg/kg鼠重。
Other AbstractThe Microcystis waterblooms were collected from the eutrophic Chao Lake from Sept.to Nov. in 1986. Only the waterblooms of M. aeruginosa from Tang-shi on Sept.8th and M.flos-aquae from Zhong-miao on Sept.9th were found toxic to mice after intraperitoneal injection bioassay. These two samples contained fast death factor (FDF) which produced by the algal cells. The minimum lethal dose (LDmin) of toxic M.aeruginosa waterbloom was 90mg/Kg of mice, and M.flos-aquae waterbloom, about 1000 mg/Kg of mice. When the injected dosage larger than the LDmin, the animals would die in 4 hours. The symptoms were identical to that of other heptotoxin-producing Microcystis blooms and strains reported from other parts of the world. The toxin of Chao Lake M.aeruginosa waterbloom was heatstable peptide, and was extracted by methanol/butanol. separated by DEAE-SephadexA 50 ion-exchange chromatography, and purified by high performance liquid chromatography (HPLC) The total toxicity recovery was about 16.36 percent, an the purity of the purified to xin was about 95.75 percent measured by HPLC. The content of toxin in M.aeruginosa cells was about 4.45 mg 7820 toxin/g dry weight alga, and the LDmin of the purified toxin was about 0.33mg/Kg of mice. On chloride acid hydrolysis the amino acid composition of the toxin was Asp(1),Clu(3), Ala(3), Leu(3), Arg(2), and was similar to that of Cyanoginosin-LR.
Document Type学位论文
Recommended Citation
GB/T 7714
张青学. 巢湖微囊藻(Microcystis)水花(waterblooms)毒性及毒素的研究[D]. 中国科学院水生生物研究所. 中国科学院水生生物研究所,1987.
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